SNU56_YEAST
ID SNU56_YEAST Reviewed; 492 AA.
AC Q03782; D6VSM1;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=56 kDa U1 small nuclear ribonucleoprotein component;
GN Name=SNU56; Synonyms=MUD10; OrderedLocusNames=YDR240C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP PROTEIN SEQUENCE OF 154-171 AND 290-304, IDENTIFICATION IN U1 SNRNP
RP COMPLEX, AND MUTAGENESIS OF SER-125.
RX PubMed=9630245;
RA Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V.,
RA Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.;
RT "A comprehensive biochemical and genetic analysis of the yeast U1 snRNP
RT reveals five novel proteins.";
RL RNA 4:374-393(1998).
RN [5]
RP RNA-BINDING.
RX PubMed=10072386; DOI=10.1101/gad.13.5.581;
RA Zhang D., Rosbash M.;
RT "Identification of eight proteins that cross-link to pre-mRNA in the yeast
RT commitment complex.";
RL Genes Dev. 13:581-592(1999).
RN [6]
RP INTERACTION WITH CBC1-CBC2.
RX PubMed=10490594; DOI=10.1128/mcb.19.10.6543;
RA Fortes P., Kufel J., Fornerod M., Polycarpou-Schwarz M., Lafontaine D.,
RA Tollervey D., Mattaj I.W.;
RT "Genetic and physical interactions involving the yeast nuclear cap-binding
RT complex.";
RL Mol. Cell. Biol. 19:6543-6553(1999).
RN [7]
RP IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=11804584; DOI=10.1016/s1097-2765(02)00436-7;
RA Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D.,
RA Abelson J.;
RT "Composition and functional characterization of the yeast spliceosomal
RT penta-snRNP.";
RL Mol. Cell 9:31-44(2002).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Component of the U1 snRNP particle, which recognizes and
CC binds the 5'-splice site of pre-mRNA. Together with other non-snRNP
CC factors, U1 snRNP forms the spliceosomal commitment complex, that
CC targets pre-mRNA to the splicing pathway.
CC -!- SUBUNIT: Component of the 18S U1 snRNP particle, a subcomplex of the
CC spliceosome. Interacts with the nuclear cap-binding complex CBC1-CBC2
CC (yCBC). Directly contacts intronic sequences of substrate pre-RNA.
CC {ECO:0000269|PubMed:10490594, ECO:0000269|PubMed:11804584,
CC ECO:0000269|PubMed:9630245}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 361 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z49701; CAA89726.1; -; Genomic_DNA.
DR EMBL; AY723783; AAU09700.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12081.1; -; Genomic_DNA.
DR PIR; S54536; S54536.
DR RefSeq; NP_010526.1; NM_001180548.1.
DR PDB; 5ZWN; EM; 3.30 A; W=1-492.
DR PDB; 6G90; EM; 4.00 A; G=1-492.
DR PDB; 6N7P; EM; 3.60 A; G=1-492.
DR PDB; 6N7R; EM; 3.20 A; G=1-492.
DR PDB; 6N7X; EM; 3.60 A; G=1-492.
DR PDB; 7OQC; EM; 4.10 A; G=1-492.
DR PDB; 7OQE; EM; 5.90 A; G=1-492.
DR PDBsum; 5ZWN; -.
DR PDBsum; 6G90; -.
DR PDBsum; 6N7P; -.
DR PDBsum; 6N7R; -.
DR PDBsum; 6N7X; -.
DR PDBsum; 7OQC; -.
DR PDBsum; 7OQE; -.
DR AlphaFoldDB; Q03782; -.
DR SMR; Q03782; -.
DR BioGRID; 32291; 293.
DR ComplexPortal; CPX-23; U1 small nuclear ribonucleoprotein complex.
DR DIP; DIP-6514N; -.
DR IntAct; Q03782; 26.
DR MINT; Q03782; -.
DR STRING; 4932.YDR240C; -.
DR iPTMnet; Q03782; -.
DR MaxQB; Q03782; -.
DR PaxDb; Q03782; -.
DR PRIDE; Q03782; -.
DR EnsemblFungi; YDR240C_mRNA; YDR240C; YDR240C.
DR GeneID; 851827; -.
DR KEGG; sce:YDR240C; -.
DR SGD; S000002648; SNU56.
DR VEuPathDB; FungiDB:YDR240C; -.
DR eggNOG; ENOG502R3P5; Eukaryota.
DR HOGENOM; CLU_554561_0_0_1; -.
DR InParanoid; Q03782; -.
DR OMA; WKNLDRI; -.
DR BioCyc; YEAST:G3O-29815-MON; -.
DR PRO; PR:Q03782; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03782; protein.
DR GO; GO:0000243; C:commitment complex; IPI:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005685; C:U1 snRNP; IDA:SGD.
DR GO; GO:0071004; C:U2-type prespliceosome; IDA:SGD.
DR GO; GO:0003729; F:mRNA binding; IPI:SGD.
DR GO; GO:0000395; P:mRNA 5'-splice site recognition; IC:ComplexPortal.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IPI:SGD.
DR InterPro; IPR043954; Snu56_snRNP.
DR Pfam; PF19097; Snu56_snRNP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..492
FT /note="56 kDa U1 small nuclear ribonucleoprotein component"
FT /id="PRO_0000232634"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..355
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 125
FT /note="S->F: Loss of function."
FT /evidence="ECO:0000269|PubMed:9630245"
FT HELIX 47..57
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 58..60
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 69..75
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 79..93
FT /evidence="ECO:0007829|PDB:6N7R"
FT TURN 95..97
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 98..103
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 112..119
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 122..138
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 157..165
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 187..189
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 194..206
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 209..219
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 231..251
FT /evidence="ECO:0007829|PDB:6N7R"
FT TURN 254..259
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 263..292
FT /evidence="ECO:0007829|PDB:6N7R"
SQ SEQUENCE 492 AA; 56498 MW; 5E0C7A1FD577808F CRC64;
MRPRRRGLAY HHTKPKGQLS QGHYPTTSND GQRRKVGNSE AFQSFDIWKN LDRIRSTKKN
AGQFIKGSLL ILPMRTEDKQ QFDECMDELH KYISKDILRC YPQKEQKDEG MLFYIVLKDF
NILDSCFVLS VLLAFQKRLW MAPSEKSYFR VPKNINLTGS FYLPKNIETG RGHIITSYRR
EQPSSSIVEV GFNVVPDFQQ FQVKACHVSK FMNELSNFFS QVEFGKCEAN VINYFKREYN
RTYSQISLAL YELPLIGDGL FDIKSYISKT RPIIETSKAQ MIKHISEMKA YNEISGLQGD
QFPRQQRPLS NSPSSNSISS SQTIEAGATS YQTQPQRHAV NKPSNVLNSS NRHSGPKTFE
DGRYSEGNKP GFMTQDEIKQ HCIGTIKASM DAVKKKSSYQ ILKTYVRCPR QNYIDIVYQN
LNDLRSKTNC NIVVLNLNNL HESQMWLESL NTTNYTIFAQ APHPSTIRVI SIGGVGEYIV
KALELILNIL EH
