ID   SNU66_SCHPO             Reviewed;         649 AA.
AC   O94538;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=U4/U6.U5 tri-snRNP-associated protein snu66;
GN   Name=snu66; ORFNames=SPAC167.03c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, INTERACTION WITH HUB1, AND SUBCELLULAR LOCATION.
RX   PubMed=15620657; DOI=10.1016/j.cub.2004.11.058;
RA   Wilkinson C.R.M., Dittmar G.A.G., Ohi M.D., Uetz P., Jones N., Finley D.;
RT   "Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and
RT   localization of an essential splicing factor in fission yeast.";
RL   Curr. Biol. 14:2283-2288(2004).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Required for efficient splicing of pre-mRNA.
CC       {ECO:0000269|PubMed:15620657}.
CC   -!- SUBUNIT: Interacts with hub1. {ECO:0000269|PubMed:15620657}.
CC   -!- INTERACTION:
CC       O94538; O94650: hub1; NbExp=2; IntAct=EBI-607744, EBI-607734;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15620657,
CC       ECO:0000269|PubMed:16823372}.
CC   -!- SIMILARITY: Belongs to the SNU66/SART1 family. {ECO:0000305}.
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DR   EMBL; CU329670; CAA22848.1; -; Genomic_DNA.
DR   PIR; T37740; T37740.
DR   RefSeq; NP_593382.1; NM_001018814.2.
DR   AlphaFoldDB; O94538; -.
DR   BioGRID; 279223; 31.
DR   IntAct; O94538; 2.
DR   STRING; 4896.SPAC167.03c.1; -.
DR   iPTMnet; O94538; -.
DR   MaxQB; O94538; -.
DR   PaxDb; O94538; -.
DR   PRIDE; O94538; -.
DR   EnsemblFungi; SPAC167.03c.1; SPAC167.03c.1:pep; SPAC167.03c.
DR   GeneID; 2542774; -.
DR   KEGG; spo:SPAC167.03c; -.
DR   PomBase; SPAC167.03c; snu66.
DR   VEuPathDB; FungiDB:SPAC167.03c; -.
DR   eggNOG; KOG2217; Eukaryota.
DR   HOGENOM; CLU_009379_2_0_1; -.
DR   InParanoid; O94538; -.
DR   OMA; EYARWEN; -.
DR   PhylomeDB; O94538; -.
DR   PRO; PR:O94538; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0005681; C:spliceosomal complex; IC:PomBase.
DR   GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IDA:PomBase.
DR   GO; GO:0000481; P:maturation of 5S rRNA; ISO:PomBase.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IMP:PomBase.
DR   InterPro; IPR045347; HIND.
DR   InterPro; IPR005011; SNU66/SART1.
DR   PANTHER; PTHR14152; PTHR14152; 1.
DR   Pfam; PF19252; HIND; 1.
DR   Pfam; PF03343; SART-1; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Ubl conjugation pathway.
FT   CHAIN           1..649
FT                   /note="U4/U6.U5 tri-snRNP-associated protein snu66"
FT                   /id="PRO_0000290651"
FT   REGION          27..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          78..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..154
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          286..327
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          433..464
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          541..570
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          49..93
FT                   /evidence="ECO:0000255"
FT   COILED          516..583
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        41..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        445..464
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   649 AA;  74734 MW;  27EC1138F612329D CRC64;
     MSGNSGASES ISIEETNRIR ISLGLKPLDI SEEKPQKELS DASVKSSYVD QEQQAYENWK
     KQEQEEINRK KEEELKSKFE KLRQKNERRR RTQGKTLAET LAEEDDQIDA NDTRAWILKM
     RNSSLNKNGN GLNFEAKNDA PRRSTLHSQN AGPNVNSSLE GMKIAHGFQD LNKNDGLVLT
     LKDADILDED NHDLLENVEM VQRKTKNERK EDNPYKPYED ENDFLQQSEA DQPSEDTFTT
     IGPQNSLTVN STIEKHKSDN KKTFGTLVSF VEPTITGSEQ SDYRQIKIKK SKKKKSKSDR
     RKRLVELDAE NENENDPSDF VLPNGQSNSD LSVEQDSAIF KEQKKMKIQK RMRELETQSF
     ADDDDLQQSI AMQRRLAQKR AKILKPEDVA EQLQNAEEVT DMTDSDTASG LIFDDTRAFV
     NSIKETENRE ALGSINEQAF EDSKDLNDTN SITGSSPTEE SNALVEDTSV DISATLEEAN
     TQQENAEDEP LVSDNVGAVL SLLRNKGVIK VSDEAKEKIQ KEEEYNKWFA RKQQARVELE
     EQRRKKKEQD RLSGKFEKMT QKEREQYAKK ENERWDKKIA EIELEQFHDY KPQVDIKYVD
     EFGVELGPKE AYKYLLSHQF HGKGSGKAKT EKRLRRIVEK EREERKPIF