SNU71_YEAST
ID SNU71_YEAST Reviewed; 620 AA.
AC P53207; D6VUE9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=U1 small nuclear ribonucleoprotein component SNU71;
GN Name=SNU71; OrderedLocusNames=YGR013W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PROTEIN SEQUENCE OF 26-38; 104-128; 191-197; 232-240; 451-461; 468-479;
RP 505-549 AND 597-613, AND IDENTIFICATION IN U1 SNRNP COMPLEX.
RX PubMed=9630245;
RA Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V.,
RA Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.;
RT "A comprehensive biochemical and genetic analysis of the yeast U1 snRNP
RT reveals five novel proteins.";
RL RNA 4:374-393(1998).
RN [5]
RP IDENTIFICATION IN U1 SNRNP COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=10504710; DOI=10.1038/13732;
RA Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., Seraphin B.;
RT "A generic protein purification method for protein complex characterization
RT and proteome exploration.";
RL Nat. Biotechnol. 17:1030-1032(1999).
RN [6]
RP FUNCTION OF THE U1 SNRNP COMPLEX.
RX PubMed=11877437; DOI=10.1074/jbc.m112460200;
RA Libri D., Duconge F., Levy L., Vinauger M.;
RT "A role for the Psi-U mismatch in the recognition of the 5' splice site of
RT yeast introns by the U1 small nuclear ribonucleoprotein particle.";
RL J. Biol. Chem. 277:18173-18181(2002).
RN [7]
RP IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=11804584; DOI=10.1016/s1097-2765(02)00436-7;
RA Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D.,
RA Abelson J.;
RT "Composition and functional characterization of the yeast spliceosomal
RT penta-snRNP.";
RL Mol. Cell 9:31-44(2002).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [9]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512 AND SER-514, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
CC -!- FUNCTION: Component of the U1 snRNP particle, which recognizes and
CC binds the 5'-splice site of pre-mRNA. Together with other non-snRNP
CC factors, U1 snRNP forms the spliceosomal commitment complex, that
CC targets pre-mRNA to the splicing pathway.
CC {ECO:0000269|PubMed:11877437}.
CC -!- SUBUNIT: Component of the 18S U1 snRNP particle, a subcomplex of the
CC spliceosome. {ECO:0000269|PubMed:10504710, ECO:0000269|PubMed:11804584,
CC ECO:0000269|PubMed:9630245}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 782 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SNU71 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z72798; CAA96996.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08110.1; -; Genomic_DNA.
DR PIR; S64304; S64304.
DR RefSeq; NP_011527.1; NM_001181142.1.
DR PDB; 5ZWN; EM; 3.30 A; X=1-619.
DR PDB; 6G90; EM; 4.00 A; J=1-620.
DR PDB; 6N7P; EM; 3.60 A; H=1-52.
DR PDB; 6N7R; EM; 3.20 A; H=1-52.
DR PDB; 6N7X; EM; 3.60 A; H=1-620.
DR PDB; 7OQC; EM; 4.10 A; J=1-620.
DR PDB; 7OQE; EM; 5.90 A; J=1-620.
DR PDBsum; 5ZWN; -.
DR PDBsum; 6G90; -.
DR PDBsum; 6N7P; -.
DR PDBsum; 6N7R; -.
DR PDBsum; 6N7X; -.
DR PDBsum; 7OQC; -.
DR PDBsum; 7OQE; -.
DR AlphaFoldDB; P53207; -.
DR SMR; P53207; -.
DR BioGRID; 33256; 114.
DR ComplexPortal; CPX-23; U1 small nuclear ribonucleoprotein complex.
DR DIP; DIP-5507N; -.
DR IntAct; P53207; 24.
DR MINT; P53207; -.
DR STRING; 4932.YGR013W; -.
DR iPTMnet; P53207; -.
DR MaxQB; P53207; -.
DR PaxDb; P53207; -.
DR PRIDE; P53207; -.
DR EnsemblFungi; YGR013W_mRNA; YGR013W; YGR013W.
DR GeneID; 852896; -.
DR KEGG; sce:YGR013W; -.
DR SGD; S000003245; SNU71.
DR VEuPathDB; FungiDB:YGR013W; -.
DR eggNOG; KOG2253; Eukaryota.
DR HOGENOM; CLU_031562_0_0_1; -.
DR InParanoid; P53207; -.
DR OMA; YDHHRSF; -.
DR BioCyc; YEAST:G3O-30740-MON; -.
DR PRO; PR:P53207; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53207; protein.
DR GO; GO:0000243; C:commitment complex; IC:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005681; C:spliceosomal complex; IC:ComplexPortal.
DR GO; GO:0005685; C:U1 snRNP; IDA:SGD.
DR GO; GO:0071004; C:U2-type prespliceosome; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IDA:SGD.
DR GO; GO:0000395; P:mRNA 5'-splice site recognition; IC:ComplexPortal.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IDA:SGD.
DR InterPro; IPR002483; PWI_dom.
DR SMART; SM00311; PWI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Cytoplasm; Direct protein sequencing;
KW mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Ribonucleoprotein; RNA-binding; Spliceosome.
FT CHAIN 1..620
FT /note="U1 small nuclear ribonucleoprotein component SNU71"
FT /id="PRO_0000202781"
FT REGION 59..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 428..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 509..537
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 296..385
FT /evidence="ECO:0000255"
FT COMPBIAS 246..260
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..337
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 338..353
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 354..373
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..448
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 512
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 514
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT STRAND 3..7
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 9..13
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 20..23
FT /evidence="ECO:0007829|PDB:6N7R"
FT STRAND 29..33
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 36..42
FT /evidence="ECO:0007829|PDB:6N7R"
FT HELIX 44..51
FT /evidence="ECO:0007829|PDB:6N7R"
SQ SEQUENCE 620 AA; 71383 MW; 2659AAA62C4993D5 CRC64;
MRDIVFVSPQ LYLSSQEGWK SDSAKSGFIP ILKNDLQRFQ DSLKHIVDAR NSLSETLLNS
NDDGSIHNSD QNTGLNKDKE ASIADNNSAN KCATSSSRYQ ELKQFLPISL DQQIHTVSLQ
GVSSSFSRGQ IESLLDHCLN LALTETQSNS ALKVEAWSSF SSFLDTQDIF IRFSKVDEDE
AFVNTLNYCK ALFAFIRKLH EDFKIELHLD LNTKEYVEDR TGTIPSVKPE KASEFYSVFK
NIEDQTDERN SKKEQLDDSS TQYKVDTNTL SDLPSDALDQ LCKDIIEFRT KVVSIEKEKK
MKSTYEESRR QRHQMQKVFD QIRKNHSGAK GSANTEEEDT NMEDEDEEDD TEDDLALEKR
KEERDLEESN RRYEDMLHQL HSNTEPKIKS IRADIMSAEN YEEHLEKNRS LYLKELLHLA
NDVHYDHHRS FKEQEERRDE EDRAKNGNAK ELAPIQLSDG KAISAGKAAA ITLPEGTVKS
ENYNADKNVS ESSEHVKIKF DFKKAIDHSV ESSSEDEGYR ESELPPTKPS ERSAAEDRLP
FTADELNIRL TNLKESRYVD ELVREFLGVY EDELVEYILE NIRVNQSKQA LLNELRETFD
EDGETIADRL WSRKEFRLGT
