SNX14_MOUSE
ID SNX14_MOUSE Reviewed; 964 AA.
AC Q8BHY8; B0V2Q4; G3UWA3;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-JUN-2015, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Sorting nexin-14;
GN Name=Snx14;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP IMPRINTING, SUBCELLULAR LOCATION, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=24859318; DOI=10.1371/journal.pone.0098383;
RA Huang H.S., Yoon B.J., Brooks S., Bakal R., Berrios J., Larsen R.S.,
RA Wallace M.L., Han J.E., Chung E.H., Zylka M.J., Philpot B.D.;
RT "Snx14 regulates neuronal excitability, promotes synaptic transmission, and
RT is imprinted in the brain of mice.";
RL PLoS ONE 9:E98383-E98383(2014).
CC -!- FUNCTION: Plays a role in maintaining normal neuronal excitability and
CC synaptic transmission. May be involved in several stages of
CC intracellular trafficking (PubMed:24859318). Required for autophagosome
CC clearance, possibly by mediating the fusion of lysosomes with
CC autophagosomes. Binds phosphatidylinositol 3,5-bisphosphate
CC (PtdIns(3,5)P2), a key component of late endosomes/lysosomes. Does not
CC bind phosphatidylinositol 3-phosphate (PtdIns(3P)) (By similarity).
CC {ECO:0000250|UniProtKB:Q9Y5W7, ECO:0000269|PubMed:24859318}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250|UniProtKB:Q9Y5W7};
CC Multi-pass membrane protein {ECO:0000305}. Late endosome membrane
CC {ECO:0000250|UniProtKB:Q9Y5W7}; Multi-pass membrane protein
CC {ECO:0000305}. Cell projection, dendrite {ECO:0000269|PubMed:24859318}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the brain, testes, and
CC lung and is present in diverse brain regions. Increases significantly
CC during neuron development and maturation.
CC {ECO:0000269|PubMed:24859318}.
CC -!- MISCELLANEOUS: The SNX14 locus is maternally imprinted.
CC {ECO:0000269|PubMed:24859318}.
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH80799.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC33716.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK049371; BAC33716.1; ALT_INIT; mRNA.
DR EMBL; AC116713; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT025673; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466522; EDL26530.1; -; Genomic_DNA.
DR EMBL; BC080799; AAH80799.1; ALT_INIT; mRNA.
DR RefSeq; NP_766514.2; NM_172926.3.
DR AlphaFoldDB; Q8BHY8; -.
DR SMR; Q8BHY8; -.
DR STRING; 10090.ENSMUSP00000130116; -.
DR iPTMnet; Q8BHY8; -.
DR PhosphoSitePlus; Q8BHY8; -.
DR MaxQB; Q8BHY8; -.
DR PaxDb; Q8BHY8; -.
DR PRIDE; Q8BHY8; -.
DR ProteomicsDB; 261594; -.
DR Antibodypedia; 3006; 81 antibodies from 17 providers.
DR DNASU; 244962; -.
DR Ensembl; ENSMUST00000239462; ENSMUSP00000159306; ENSMUSG00000032422.
DR GeneID; 244962; -.
DR KEGG; mmu:244962; -.
DR UCSC; uc009qyl.2; mouse.
DR CTD; 57231; -.
DR MGI; MGI:2155664; Snx14.
DR VEuPathDB; HostDB:ENSMUSG00000032422; -.
DR eggNOG; KOG2101; Eukaryota.
DR GeneTree; ENSGT00950000182856; -.
DR InParanoid; Q8BHY8; -.
DR OrthoDB; 257375at2759; -.
DR TreeFam; TF324055; -.
DR BioGRID-ORCS; 244962; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Snx14; mouse.
DR PRO; PR:Q8BHY8; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8BHY8; protein.
DR Bgee; ENSMUSG00000032422; Expressed in spermatocyte and 250 other tissues.
DR ExpressionAtlas; Q8BHY8; baseline and differential.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0035091; F:phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; ISS:UniProtKB.
DR GO; GO:0097352; P:autophagosome maturation; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd06877; PX_SNX14; 1.
DR CDD; cd08722; RGS_SNX14; 1.
DR Gene3D; 1.10.167.10; -; 1.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR003114; Phox_assoc.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR InterPro; IPR037436; SNX14_PX.
DR InterPro; IPR037892; SNX14_RGS.
DR InterPro; IPR013937; Sorting_nexin_C.
DR Pfam; PF08628; Nexin_C; 1.
DR Pfam; PF00787; PX; 1.
DR Pfam; PF02194; PXA; 1.
DR Pfam; PF00615; RGS; 1.
DR SMART; SM00312; PX; 1.
DR SMART; SM00313; PXA; 1.
DR SMART; SM00315; RGS; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
DR PROSITE; PS51207; PXA; 1.
DR PROSITE; PS50132; RGS; 1.
PE 1: Evidence at protein level;
KW Cell projection; Endosome; Lysosome; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..964
FT /note="Sorting nexin-14"
FT /id="PRO_0000236201"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 157..331
FT /note="PXA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147,
FT ECO:0000255|PROSITE-ProRule:PRU00553"
FT DOMAIN 363..495
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT DOMAIN 588..708
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 566
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5W7"
FT CONFLICT 7
FT /note="G -> R (in Ref. 4; AAH80799)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 964 AA; 111865 MW; 3FF60D12EF8FBBC4 CRC64;
MWLRRRGLGV PSASASEGGT SSPAWTEMGS WVRTICGRLK QRLRLDVGRE ICRQYPLFCF
LLLCLSVASL LLNRYLHVLM IFWSFVAGVV TFYCSLGPDS LLPNIFFTIK YKPKQLGLQE
LFPQGHSCAV CGKVKCKRHR PSLLLENYQP WLDLKVSSKV DASLSEVLEL VLENFVYPWY
RDVTDDESFV DELRITLRFF ASVLVRRIHK VDIPSIITKK LLKAAMKHIE VIVKARQKVK
NTEYLQQAAL EEYGPELHVA LRSRRDELQY LRKLTELLFP YILPPKATDC RSLTLLIREI
LSGSVLLPSL DFLADPDTVN HLLIIFIDDS PPEKATEPAS PLVPFLQKFA EPRNKKPSVL
KLELKQIREQ QDLLFRFMNF LKQEGAVHVL QFCLTVEEFN DRILRPELSN DEMLSLHEEL
QKIYKTYCLD ESIDKIRFDP FIVEEIQRIA EGPYIDVVKL QTMRCLFEAY EHVLSLLENV
FTPMFCHSDE YFRQLLRGAE SPTRNSKFNR STQKRGESFG ISRIGSKIKG VFKSTTMEGA
VLPNYGVAEG EDDFIEEGIV VMEDDSPVEA VSTPNTPRNL AAWKISIPYV DFFEDPSSER
KEKKERIPVF CIDVERNDRR AVGHEPEHWS VYRRYLEFYV LESKLTEFHG TFPDAQLPSK
RIIGPKNYEF LKSKREEFQE YLQKLVQHPE LSNSQLLADF LSPNGGETQF LDKILPDVNL
GKIIKSVPGK LMKEKGQHLE PFIMSFINSC ESPKPKPSRP ELTILSPTSE NNKKLFNDLF
KNNANRAENT ERKQNQNYFM EVMTVDGVYD YLMYVGRVVF QVPDWLHHLL MGTRILFKNT
LEMYTDYYLQ CKLEQLFQEH RLVSLITLLR DAIFCENTEP RSLQDKQKGA KQTFEEMMNY
IPDLIVKCIG EETKYESIRL LFDGLQQPVL NKQLTYVLLD IVIQELFPEL NKVQKEATSM
TSWM
