SNX20_HUMAN
ID SNX20_HUMAN Reviewed; 316 AA.
AC Q7Z614; A8K9D5; Q08E98; Q6P4H2; Q8IV59;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Sorting nexin-20;
DE AltName: Full=Selectin ligand-interactor cytoplasmic 1 {ECO:0000303|PubMed:18196517};
DE Short=SLIC-1 {ECO:0000303|PubMed:18196517};
GN Name=SNX20; Synonyms=SLIC1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION
RP WITH SELPLG, AND MUTAGENESIS OF ARG-116.
RX PubMed=18196517; DOI=10.1002/eji.200737777;
RA Schaff U.Y., Shih H.H., Lorenz M., Sako D., Kriz R., Milarski K., Bates B.,
RA Tchernychev B., Shaw G.D., Simon S.I.;
RT "SLIC-1/sorting nexin 20: a novel sorting nexin that directs subcellular
RT distribution of PSGL-1.";
RL Eur. J. Immunol. 38:550-564(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC TISSUE=Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4), AND VARIANT
RP LEU-35.
RC TISSUE=Leukocyte;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in cellular vesicle trafficking. Has been
CC proposed to function as a sorting protein that targets SELPLG into
CC endosomes, but has no effect on SELPLG internalization from the cell
CC surface, or on SELPLG-mediated cell-cell adhesion.
CC {ECO:0000305|PubMed:18196517}.
CC -!- SUBUNIT: Interacts with SELPLG (PubMed:18196517). Interaction with
CC SELPLG is controversial. {ECO:0000269|PubMed:18196517, ECO:0000305}.
CC -!- INTERACTION:
CC Q7Z614; P13196: ALAS1; NbExp=3; IntAct=EBI-744896, EBI-3905054;
CC Q7Z614; Q8IZU0: FAM9B; NbExp=4; IntAct=EBI-744896, EBI-10175124;
CC Q7Z614; Q53SE7: FLJ13057; NbExp=3; IntAct=EBI-744896, EBI-10172181;
CC Q7Z614; Q53G59: KLHL12; NbExp=4; IntAct=EBI-744896, EBI-740929;
CC Q7Z614; P43364-2: MAGEA11; NbExp=3; IntAct=EBI-744896, EBI-10178634;
CC Q7Z614; Q14242: SELPLG; NbExp=5; IntAct=EBI-744896, EBI-1030190;
CC Q7Z614; Q9Y6A5: TACC3; NbExp=3; IntAct=EBI-744896, EBI-2554984;
CC Q7Z614; Q9UBB9: TFIP11; NbExp=4; IntAct=EBI-744896, EBI-1105213;
CC Q7Z614-3; P23560-2: BDNF; NbExp=3; IntAct=EBI-12336127, EBI-12275524;
CC Q7Z614-3; Q53G59: KLHL12; NbExp=3; IntAct=EBI-12336127, EBI-740929;
CC Q7Z614-3; P43364: MAGEA11; NbExp=3; IntAct=EBI-12336127, EBI-739552;
CC Q7Z614-3; P16284: PECAM1; NbExp=3; IntAct=EBI-12336127, EBI-716404;
CC Q7Z614-3; P50454: SERPINH1; NbExp=3; IntAct=EBI-12336127, EBI-350723;
CC Q7Z614-3; O95238: SPDEF; NbExp=3; IntAct=EBI-12336127, EBI-12811275;
CC Q7Z614-3; P37173: TGFBR2; NbExp=3; IntAct=EBI-12336127, EBI-296151;
CC Q7Z614-3; P08670: VIM; NbExp=3; IntAct=EBI-12336127, EBI-353844;
CC -!- SUBCELLULAR LOCATION: Early endosome membrane
CC {ECO:0000269|PubMed:18196517}; Peripheral membrane protein
CC {ECO:0000269|PubMed:18196517}; Cytoplasmic side
CC {ECO:0000269|PubMed:18196517}. Cell membrane
CC {ECO:0000269|PubMed:18196517}. Cytoplasm {ECO:0000269|PubMed:18196517}.
CC Nucleus {ECO:0000269|PubMed:18196517}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q7Z614-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7Z614-2; Sequence=VSP_032428;
CC Name=3;
CC IsoId=Q7Z614-3; Sequence=VSP_032425, VSP_032429;
CC Name=4;
CC IsoId=Q7Z614-4; Sequence=VSP_032426, VSP_032427;
CC -!- DOMAIN: The PX domain binds phosphatidylinositol 3-phosphate which is
CC necessary for localization to the endosomes.
CC {ECO:0000305|PubMed:18196517}.
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
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DR EMBL; AY302441; AAP58362.1; -; mRNA.
DR EMBL; AK292650; BAF85339.1; -; mRNA.
DR EMBL; AK292705; BAF85394.1; -; mRNA.
DR EMBL; CH471092; EAW82763.1; -; Genomic_DNA.
DR EMBL; BC063423; AAH63423.1; -; mRNA.
DR EMBL; BC119626; AAI19627.1; -; mRNA.
DR CCDS; CCDS10744.1; -. [Q7Z614-3]
DR CCDS; CCDS10745.1; -. [Q7Z614-1]
DR CCDS; CCDS45481.1; -. [Q7Z614-4]
DR RefSeq; NP_001138444.1; NM_001144972.1. [Q7Z614-4]
DR RefSeq; NP_699168.1; NM_153337.2. [Q7Z614-3]
DR RefSeq; NP_878274.1; NM_182854.3. [Q7Z614-1]
DR AlphaFoldDB; Q7Z614; -.
DR SMR; Q7Z614; -.
DR BioGRID; 125867; 25.
DR IntAct; Q7Z614; 23.
DR STRING; 9606.ENSP00000332062; -.
DR iPTMnet; Q7Z614; -.
DR PhosphoSitePlus; Q7Z614; -.
DR BioMuta; SNX20; -.
DR DMDM; 74713704; -.
DR jPOST; Q7Z614; -.
DR MassIVE; Q7Z614; -.
DR PaxDb; Q7Z614; -.
DR PeptideAtlas; Q7Z614; -.
DR PRIDE; Q7Z614; -.
DR ProteomicsDB; 69364; -. [Q7Z614-1]
DR ProteomicsDB; 69365; -. [Q7Z614-2]
DR ProteomicsDB; 69366; -. [Q7Z614-3]
DR ProteomicsDB; 69367; -. [Q7Z614-4]
DR Antibodypedia; 28297; 94 antibodies from 18 providers.
DR DNASU; 124460; -.
DR Ensembl; ENST00000300590.7; ENSP00000300590.3; ENSG00000167208.16. [Q7Z614-3]
DR Ensembl; ENST00000330943.9; ENSP00000332062.4; ENSG00000167208.16. [Q7Z614-1]
DR Ensembl; ENST00000423026.6; ENSP00000388875.2; ENSG00000167208.16. [Q7Z614-4]
DR Ensembl; ENST00000568993.5; ENSP00000454863.1; ENSG00000167208.16. [Q7Z614-3]
DR GeneID; 124460; -.
DR KEGG; hsa:124460; -.
DR MANE-Select; ENST00000330943.9; ENSP00000332062.4; NM_182854.4; NP_878274.1.
DR UCSC; uc002egi.4; human. [Q7Z614-1]
DR CTD; 124460; -.
DR DisGeNET; 124460; -.
DR GeneCards; SNX20; -.
DR HGNC; HGNC:30390; SNX20.
DR HPA; ENSG00000167208; Tissue enhanced (bone marrow, lymphoid tissue).
DR MIM; 613281; gene.
DR neXtProt; NX_Q7Z614; -.
DR OpenTargets; ENSG00000167208; -.
DR PharmGKB; PA162404311; -.
DR VEuPathDB; HostDB:ENSG00000167208; -.
DR eggNOG; KOG2101; Eukaryota.
DR GeneTree; ENSGT00530000063759; -.
DR HOGENOM; CLU_160093_0_0_1; -.
DR InParanoid; Q7Z614; -.
DR OMA; ATRCVRH; -.
DR OrthoDB; 1322681at2759; -.
DR PhylomeDB; Q7Z614; -.
DR TreeFam; TF326807; -.
DR PathwayCommons; Q7Z614; -.
DR SignaLink; Q7Z614; -.
DR BioGRID-ORCS; 124460; 7 hits in 1071 CRISPR screens.
DR ChiTaRS; SNX20; human.
DR GenomeRNAi; 124460; -.
DR Pharos; Q7Z614; Tdark.
DR PRO; PR:Q7Z614; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q7Z614; protein.
DR Bgee; ENSG00000167208; Expressed in granulocyte and 143 other tissues.
DR ExpressionAtlas; Q7Z614; baseline and differential.
DR Genevisible; Q7Z614; HS.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; IBA:GO_Central.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; ISS:UniProtKB.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR039937; SNX20/SNX21.
DR PANTHER; PTHR20939; PTHR20939; 1.
DR Pfam; PF00787; PX; 1.
DR SMART; SM00312; PX; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Endosome; Lipid-binding;
KW Membrane; Nucleus; Phosphoprotein; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..316
FT /note="Sorting nexin-20"
FT /id="PRO_0000325820"
FT DOMAIN 74..191
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT REGION 23..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 116
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250|UniProtKB:Q6P4T1"
FT BINDING 118
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250|UniProtKB:Q96L94"
FT BINDING 143
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250|UniProtKB:Q96L94"
FT BINDING 157
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250|UniProtKB:Q6P4T1"
FT MOD_RES 3
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5BK61"
FT VAR_SEQ 95..129
FT /note="VYQIIVIQTGSFDNNKAVLERRYSDFAKLQKALLK -> MGKSRPGEMTYPG
FT SRGETGTAPEPDPRCPRQSDML (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_032425"
FT VAR_SEQ 95..102
FT /note="VYQIIVIQ -> APAPDATP (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_032426"
FT VAR_SEQ 103..316
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_032427"
FT VAR_SEQ 121..284
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032428"
FT VAR_SEQ 130..316
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_032429"
FT VARIANT 35
FT /note="P -> L (in dbSNP:rs1131716)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_039927"
FT MUTAGEN 116
FT /note="R->Q: Decreased binding activity to all
FT phospholipids."
FT /evidence="ECO:0000269|PubMed:18196517"
SQ SEQUENCE 316 AA; 36178 MW; 48D06CF4490907F4 CRC64;
MASPEHPGSP GCMGPITQCT ARTQQEAPAT GPDLPHPGPD GHLDTHSGLS SNSSMTTREL
QQYWQNQKCR WKHVKLLFEI ASARIEERKV SKFVVYQIIV IQTGSFDNNK AVLERRYSDF
AKLQKALLKT FREEIEDVEF PRKHLTGNFA EEMICERRRA LQEYLGLLYA IRCVRRSREF
LDFLTRPELR EAFGCLRAGQ YPRALELLLR VLPLQEKLTA HCPAAAVPAL CAVLLCHRDL
DRPAEAFAAG ERALQRLQAR EGHRYYAPLL DAMVRLAYAL GKDFVTLQER LEESQLRRPT
PRGITLKELT VREYLH
