SNX25_HUMAN
ID SNX25_HUMAN Reviewed; 840 AA.
AC Q9H3E2; Q3ZT30; Q8N6K3;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 04-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Sorting nexin-25;
GN Name=SNX25; ORFNames=MSTP043;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Hao X., Chang Z., Zhu S.;
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 275-840 (ISOFORM 1).
RC TISSUE=Heart;
RA Liu B., Liu Y.Q., Wang X.Y., Zhao B., Sheng H., Zhao X.W., Liu S., Xu Y.Y.,
RA Ye J., Song L., Gao Y., Zhang C.L., Zhang J., Wei Y.J., Cao H.Q., Zhao Y.,
RA Liu L.S., Ding J.F., Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C.,
RA Zhao M.S., Hui R.T.;
RL Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 303-840 (ISOFORM 1).
RA Hong W.;
RT "MSTP043 as a new member (SNX25) of the sorting nexin family.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14975938; DOI=10.1165/rcmb.2003-0238oc;
RA Bard M.P., Hegmans J.P., Hemmes A., Luider T.M., Willemsen R.,
RA Severijnen L.A., van Meerbeeck J.P., Burgers S.A., Hoogsteden H.C.,
RA Lambrecht B.N.;
RT "Proteomic analysis of exosomes isolated from human malignant pleural
RT effusions.";
RL Am. J. Respir. Cell Mol. Biol. 31:114-121(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-665, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT networks.";
RL Cell 127:635-648(2006).
CC -!- FUNCTION: May be involved in several stages of intracellular
CC trafficking. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:14975938};
CC Peripheral membrane protein {ECO:0000269|PubMed:14975938}.
CC Note=Detected in endosome-derived secreted vesicles (exosomes) from
CC malignant pleural effusions.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H3E2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H3E2-2; Sequence=VSP_057156, VSP_057157;
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG39294.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY601647; AAT98627.1; -; mRNA.
DR EMBL; BC029868; AAH29868.2; -; mRNA.
DR EMBL; AF113223; AAG39294.1; ALT_INIT; mRNA.
DR EMBL; AY044656; AAK98770.1; -; mRNA.
DR CCDS; CCDS34116.1; -. [Q9H3E2-1]
DR RefSeq; NP_001304710.1; NM_001317781.1. [Q9H3E2-1]
DR PDB; 5WOE; NMR; -; A=506-628.
DR PDBsum; 5WOE; -.
DR AlphaFoldDB; Q9H3E2; -.
DR SMR; Q9H3E2; -.
DR BioGRID; 123802; 19.
DR IntAct; Q9H3E2; 5.
DR STRING; 9606.ENSP00000426255; -.
DR iPTMnet; Q9H3E2; -.
DR PhosphoSitePlus; Q9H3E2; -.
DR BioMuta; SNX25; -.
DR DMDM; 212276473; -.
DR EPD; Q9H3E2; -.
DR jPOST; Q9H3E2; -.
DR MassIVE; Q9H3E2; -.
DR MaxQB; Q9H3E2; -.
DR PaxDb; Q9H3E2; -.
DR PeptideAtlas; Q9H3E2; -.
DR PRIDE; Q9H3E2; -.
DR ProteomicsDB; 80704; -. [Q9H3E2-1]
DR Antibodypedia; 28920; 152 antibodies from 27 providers.
DR DNASU; 83891; -.
DR Ensembl; ENST00000264694.13; ENSP00000264694.8; ENSG00000109762.17. [Q9H3E2-1]
DR Ensembl; ENST00000504273.5; ENSP00000426255.1; ENSG00000109762.17. [Q9H3E2-1]
DR Ensembl; ENST00000618785.4; ENSP00000483653.1; ENSG00000109762.17. [Q9H3E2-2]
DR GeneID; 83891; -.
DR KEGG; hsa:83891; -.
DR UCSC; uc003ixh.4; human. [Q9H3E2-1]
DR CTD; 83891; -.
DR DisGeNET; 83891; -.
DR GeneCards; SNX25; -.
DR HGNC; HGNC:21883; SNX25.
DR HPA; ENSG00000109762; Low tissue specificity.
DR neXtProt; NX_Q9H3E2; -.
DR OpenTargets; ENSG00000109762; -.
DR PharmGKB; PA134988505; -.
DR VEuPathDB; HostDB:ENSG00000109762; -.
DR eggNOG; KOG2101; Eukaryota.
DR GeneTree; ENSGT00950000182856; -.
DR HOGENOM; CLU_005899_0_1_1; -.
DR InParanoid; Q9H3E2; -.
DR OrthoDB; 1388993at2759; -.
DR PhylomeDB; Q9H3E2; -.
DR TreeFam; TF324055; -.
DR PathwayCommons; Q9H3E2; -.
DR SignaLink; Q9H3E2; -.
DR BioGRID-ORCS; 83891; 16 hits in 1070 CRISPR screens.
DR ChiTaRS; SNX25; human.
DR GenomeRNAi; 83891; -.
DR Pharos; Q9H3E2; Tbio.
DR PRO; PR:Q9H3E2; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q9H3E2; protein.
DR Bgee; ENSG00000109762; Expressed in oocyte and 182 other tissues.
DR ExpressionAtlas; Q9H3E2; baseline and differential.
DR Genevisible; Q9H3E2; HS.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0035091; F:phosphatidylinositol binding; IBA:GO_Central.
DR GO; GO:0060394; P:negative regulation of pathway-restricted SMAD protein phosphorylation; IMP:UniProtKB.
DR GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0032801; P:receptor catabolic process; IMP:UniProtKB.
DR CDD; cd06878; PX_SNX25; 1.
DR Gene3D; 1.10.167.10; -; 1.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR003114; Phox_assoc.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR016137; RGS.
DR InterPro; IPR036305; RGS_sf.
DR InterPro; IPR044926; RGS_subdomain_2.
DR InterPro; IPR034905; SNX25.
DR InterPro; IPR037899; SNX25_PX.
DR InterPro; IPR013937; Sorting_nexin_C.
DR PANTHER; PTHR22775:SF29; PTHR22775:SF29; 1.
DR Pfam; PF08628; Nexin_C; 1.
DR Pfam; PF00787; PX; 1.
DR Pfam; PF02194; PXA; 1.
DR Pfam; PF00615; RGS; 1.
DR SMART; SM00312; PX; 1.
DR SMART; SM00313; PXA; 1.
DR SMART; SM00315; RGS; 1.
DR SUPFAM; SSF48097; SSF48097; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
DR PROSITE; PS51207; PXA; 1.
DR PROSITE; PS50132; RGS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Coiled coil; Endosome; Membrane;
KW Phosphoprotein; Protein transport; Reference proteome; Transport.
FT CHAIN 1..840
FT /note="Sorting nexin-25"
FT /id="PRO_0000213875"
FT DOMAIN 1..164
FT /note="PXA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147,
FT ECO:0000255|PROSITE-ProRule:PRU00553"
FT DOMAIN 287..401
FT /note="RGS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00171"
FT DOMAIN 508..628
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT COILED 434..499
FT /evidence="ECO:0000255"
FT MOD_RES 665
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17081983"
FT VAR_SEQ 1..229
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_057156"
FT VAR_SEQ 604..658
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_057157"
FT VARIANT 318
FT /note="E -> K (in dbSNP:rs35700132)"
FT /id="VAR_047057"
FT VARIANT 586
FT /note="I -> V (in dbSNP:rs3756275)"
FT /id="VAR_047058"
FT VARIANT 725
FT /note="T -> I (in dbSNP:rs34120554)"
FT /id="VAR_047059"
FT STRAND 510..522
FT /evidence="ECO:0007829|PDB:5WOE"
FT STRAND 525..536
FT /evidence="ECO:0007829|PDB:5WOE"
FT STRAND 545..552
FT /evidence="ECO:0007829|PDB:5WOE"
FT HELIX 553..566
FT /evidence="ECO:0007829|PDB:5WOE"
FT HELIX 569..572
FT /evidence="ECO:0007829|PDB:5WOE"
FT STRAND 582..584
FT /evidence="ECO:0007829|PDB:5WOE"
FT HELIX 588..607
FT /evidence="ECO:0007829|PDB:5WOE"
FT HELIX 609..613
FT /evidence="ECO:0007829|PDB:5WOE"
FT HELIX 615..621
FT /evidence="ECO:0007829|PDB:5WOE"
SQ SEQUENCE 840 AA; 97945 MW; D75682A660DEF124 CRC64;
MDKALKEVFD YSYRDYILSW YGNLSRDEGQ LYHLLLEDFW EIARQLHHRL SHVDVVKVVC
NDVVRTLLTH FCDLKAANAR HEEQPRPFVL HACLRNSDDE VRFLQTCSRV LVFCLLPSKD
VQSLSLRIML AEILTTKVLK PVVELLSNPD YINQMLLAQL AYREQMNEHH KRAYTYAPSY
EDFIKLINSN SDVEFLKQLR YQIVVEIIQA TTISSFPQLK RHKGKETAAM KADLLRARNM
KRYINQLTVA KKQCEKRIRI LGGPAYDQQE DGALDEGEGP QSQKILQFED ILANTFYREH
FGMYMERMDK RALISFWESV EHLKNANKNE IPQLVGEIYQ NFFVESKEIS VEKSLYKEIQ
QCLVGNKGIE VFYKIQEDVY ETLKDRYYPS FIVSDLYEKL LIKEEEKHAS QMISNKDEMG
PRDEAGEEAV DDGTNQINEQ ASFAVNKLRE LNEKLEYKRQ ALNSIQNAPK PDKKIVSKLK
DEIILIEKER TDLQLHMART DWWCENLGMW KASITSGEVT EENGEQLPCY FVMVSLQEVG
GVETKNWTVP RRLSEFQNLH RKLSECVPSL KKVQLPSLSK LPFKSIDQKF MEKSKNQLNK
FLQNLLSDER LCQSEALYAF LSPSPDYLKV IDVQGKKNSF SLSSFLERLP RDFFSHQEEE
TEEDSDLSDY GDDVDGRKDA LAEPCFMLIG EIFELRGMFK WVRRTLIALV QVTFGRTINK
QIRDTVSWIF SEQMLVYYIN IFRDAFWPNG KLAPPTTIRS KEQSQETKQR AQQKLLENIP
DMLQSLVGQQ NARHGIIKIF NALQETRANK HLLYALMELL LIELCPELRV HLDQLKAGQV
