SNX8_MOUSE
ID SNX8_MOUSE Reviewed; 459 AA.
AC Q8CFD4; Q8BLI6; Q8BUQ7;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Sorting nexin-8;
GN Name=Snx8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-459.
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in several stages of intracellular
CC trafficking. May play a role in intracellular protein transport from
CC early endosomes to the trans-Golgi network (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Early endosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC Note=Colocalizes with retromer components. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sorting nexin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC32202.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE28549.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC037599; AAH37599.1; -; mRNA.
DR EMBL; AK045061; BAC32202.1; ALT_INIT; mRNA.
DR EMBL; AK082871; BAC38662.1; -; mRNA.
DR EMBL; AK148429; BAE28549.1; ALT_INIT; mRNA.
DR CCDS; CCDS39356.1; -.
DR RefSeq; NP_758481.1; NM_172277.3.
DR AlphaFoldDB; Q8CFD4; -.
DR SMR; Q8CFD4; -.
DR BioGRID; 231175; 4.
DR IntAct; Q8CFD4; 2.
DR MINT; Q8CFD4; -.
DR STRING; 10090.ENSMUSP00000031539; -.
DR iPTMnet; Q8CFD4; -.
DR PhosphoSitePlus; Q8CFD4; -.
DR EPD; Q8CFD4; -.
DR MaxQB; Q8CFD4; -.
DR PaxDb; Q8CFD4; -.
DR PeptideAtlas; Q8CFD4; -.
DR PRIDE; Q8CFD4; -.
DR ProteomicsDB; 261545; -.
DR Antibodypedia; 24341; 290 antibodies from 28 providers.
DR DNASU; 231834; -.
DR Ensembl; ENSMUST00000031539; ENSMUSP00000031539; ENSMUSG00000029560.
DR GeneID; 231834; -.
DR KEGG; mmu:231834; -.
DR UCSC; uc009ahp.1; mouse.
DR CTD; 29886; -.
DR MGI; MGI:2443816; Snx8.
DR VEuPathDB; HostDB:ENSMUSG00000029560; -.
DR eggNOG; KOG2273; Eukaryota.
DR GeneTree; ENSGT00460000041594; -.
DR InParanoid; Q8CFD4; -.
DR OrthoDB; 793604at2759; -.
DR PhylomeDB; Q8CFD4; -.
DR TreeFam; TF314082; -.
DR BioGRID-ORCS; 231834; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Snx8; mouse.
DR PRO; PR:Q8CFD4; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8CFD4; protein.
DR Bgee; ENSMUSG00000029560; Expressed in yolk sac and 206 other tissues.
DR ExpressionAtlas; Q8CFD4; baseline and differential.
DR Genevisible; Q8CFD4; MM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0030904; C:retromer complex; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0035091; F:phosphatidylinositol binding; ISS:UniProtKB.
DR GO; GO:0034498; P:early endosome to Golgi transport; ISS:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; ISS:UniProtKB.
DR CDD; cd06866; PX_SNX8_Mvp1p_like; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 3.30.1520.10; -; 1.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR001683; PX_dom.
DR InterPro; IPR036871; PX_dom_sf.
DR InterPro; IPR028662; SNX8/Mvp1.
DR InterPro; IPR035704; SNX8/Mvp1_PX.
DR InterPro; IPR045734; Snx8_BAR_dom.
DR PANTHER; PTHR46571; PTHR46571; 1.
DR Pfam; PF00787; PX; 1.
DR Pfam; PF19566; Snx8_BAR_dom; 1.
DR SMART; SM00312; PX; 1.
DR SUPFAM; SSF64268; SSF64268; 1.
DR PROSITE; PS50195; PX; 1.
PE 1: Evidence at protein level;
KW Endosome; Lipid-binding; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..459
FT /note="Sorting nexin-8"
FT /id="PRO_0000236199"
FT DOMAIN 68..176
FT /note="PX"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00147"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 104
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 130
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT BINDING 143
FT /ligand="a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
FT inositol-3-phosphate)"
FT /ligand_id="ChEBI:CHEBI:58088"
FT /evidence="ECO:0000250"
FT MOD_RES 446
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y5X2"
FT MOD_RES 450
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 222
FT /note="N -> Y (in Ref. 2; BAC38662)"
FT /evidence="ECO:0000305"
FT CONFLICT 366
FT /note="L -> V (in Ref. 2; BAC38662)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 52059 MW; 5E0BE9C1AB054454 CRC64;
MTGRAMDPLP SPAVAAAAEA EADEEADPPA TGPRTSQVTE WRALDPGRMQ MPQGNPLLLS
YTLQELLAKD TVQVELIPEK KGLFLKHVEY EVSSQRFKSS VYRRYNDFVV FHEVLLHKFP
YRMVPALPPK RVLGADREFI EGRRRALKRF INLVARHPPF SEDVLLKLFL SFSGSDVQHK
LKEAAQCVGD EFMNCKLAAR AKDFLPADIQ TQFAMSRELI RNVYNSFYKL RDRAERIASR
AIDNAADLLI FGKELSALGS DTTPLPSWAA LHLSTWGSLK QALKGLSVEF ALLADRAAQQ
GKKEENDVVE KLNLFLDLLQ SYKDLCERHE KGVLHKHQRA LHKYGLMKRQ MMSAAHGREP
ESVEQLESRI VEQENVIQTM ELRNYFSLYC LHQETQLVHV YLPLTSHILG AFVNSQIQGH
KEMSKVWNDL KPKLSCLFAG PHSVLTPPRS PQEDGVCPH
