SO1A1_RAT
ID SO1A1_RAT Reviewed; 670 AA.
AC P46720;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Solute carrier organic anion transporter family member 1A1;
DE AltName: Full=Organic anion-transporting polypeptide 1;
DE Short=OATP-1;
DE AltName: Full=Sodium-independent organic anion transporter 1;
DE AltName: Full=Solute carrier family 21 member 1;
GN Name=Slco1a1; Synonyms=Oatp1, Oatp1a1, Slc21a1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND GLYCOSYLATION.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8278353; DOI=10.1073/pnas.91.1.133;
RA Jacquemin E., Hagenbuch B., Stieger B., Wolkoff A.W., Meier P.J.;
RT "Expression cloning of a rat liver Na(+)-independent organic anion
RT transporter.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:133-137(1994).
RN [2]
RP INTERACTION WITH PDZK1, AND MUTAGENESIS OF 666-LYS--LEU-670.
RX PubMed=15994332; DOI=10.1074/jbc.m503969200;
RA Wang P., Wang J.J., Xiao Y., Murray J.W., Novikoff P.M., Angeletti R.H.,
RA Orr G.A., Lan D., Silver D.L., Wolkoff A.W.;
RT "Interaction with PDZK1 is required for expression of organic anion
RT transporting protein 1A1 on the hepatocyte surface.";
RL J. Biol. Chem. 280:30143-30149(2005).
RN [3]
RP PHOSPHORYLATION AT SER-634 AND SER-635.
RX PubMed=16519530; DOI=10.1021/bi052437v;
RA Xiao Y., Nieves E., Angeletti R.H., Orr G.A., Wolkoff A.W.;
RT "Rat organic anion transporting protein 1A1 (Oatp1a1): purification and
RT phosphopeptide assignment.";
RL Biochemistry 45:3357-3369(2006).
RN [4]
RP SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY, AND GLYCOSYLATION AT ASN-124;
RP ASN-135 AND ASN-492.
RX PubMed=18308854; DOI=10.1152/ajpgi.00584.2007;
RA Wang P., Hata S., Xiao Y., Murray J.W., Wolkoff A.W.;
RT "Topological assessment of oatp1a1: a 12-transmembrane domain integral
RT membrane protein with three N-linked carbohydrate chains.";
RL Am. J. Physiol. 294:G1052-G1059(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Mediates the Na(+)-independent transport of organic anions
CC such as bromosulfobromophthalein (BSP) and conjugated (taurocholate)
CC and unconjugated (cholate) bile acids.
CC -!- SUBUNIT: Binds to PDZK1. Interaction with PDZK1 is required for
CC expression on hepatocyte surface.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:18308854}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:18308854}.
CC -!- TISSUE SPECIFICITY: Highly expressed in liver and kidney, and at lower
CC levels in brain, lung, skeletal muscle and proximal colon.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:18308854,
CC ECO:0000269|PubMed:8278353}.
CC -!- SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family.
CC {ECO:0000305}.
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DR EMBL; L19031; AAA16451.1; -; mRNA.
DR PIR; A49580; A49580.
DR RefSeq; NP_058807.1; NM_017111.1.
DR AlphaFoldDB; P46720; -.
DR SMR; P46720; -.
DR STRING; 10116.ENSRNOP00000040752; -.
DR BindingDB; P46720; -.
DR ChEMBL; CHEMBL1781859; -.
DR TCDB; 2.A.60.1.1; the organo anion transporter (oat) family.
DR GlyGen; P46720; 3 sites.
DR iPTMnet; P46720; -.
DR PhosphoSitePlus; P46720; -.
DR PaxDb; P46720; -.
DR PRIDE; P46720; -.
DR Ensembl; ENSRNOT00000102966; ENSRNOP00000078030; ENSRNOG00000036984.
DR GeneID; 50572; -.
DR KEGG; rno:50572; -.
DR CTD; 28248; -.
DR RGD; 3700; Slco1a1.
DR eggNOG; KOG3626; Eukaryota.
DR GeneTree; ENSGT01050000244856; -.
DR HOGENOM; CLU_008954_4_0_1; -.
DR InParanoid; P46720; -.
DR OMA; PETASEW; -.
DR OrthoDB; 1029129at2759; -.
DR PhylomeDB; P46720; -.
DR SABIO-RK; P46720; -.
DR PRO; PR:P46720; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000036984; Expressed in adult mammalian kidney and 2 other tissues.
DR GO; GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; TAS:RGD.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015721; P:bile acid and bile salt transport; IBA:GO_Central.
DR GO; GO:0015711; P:organic anion transport; IEP:RGD.
DR GO; GO:0035634; P:response to stilbenoid; IEA:Ensembl.
DR GO; GO:0033574; P:response to testosterone; IEP:RGD.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004156; OATP.
DR PANTHER; PTHR11388; PTHR11388; 1.
DR Pfam; PF07648; Kazal_2; 1.
DR Pfam; PF03137; OATP; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00805; oat; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion transport; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..670
FT /note="Solute carrier organic anion transporter family
FT member 1A1"
FT /id="PRO_0000191041"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..40
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..59
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..155
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..184
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..242
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..267
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..333
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..353
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..377
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..405
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 406..513
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 514..536
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 537..545
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 546..571
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 572..605
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..623
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 624..670
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 433..488
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT REGION 645..670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 634
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:16519530"
FT MOD_RES 635
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:16519530"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18308854"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18308854"
FT CARBOHYD 492
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18308854"
FT DISULFID 439..469
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 445..465
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 454..486
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT MUTAGEN 666..670
FT /note="Missing: Abolishes binding to PDZK1."
FT /evidence="ECO:0000269|PubMed:15994332"
SQ SEQUENCE 670 AA; 74178 MW; 3D53A4E8E1E21536 CRC64;
MEETEKKIAT QEGRLFSKMK VFLLSLTCAC LTKSLSGVYM NSMLTQIERQ FDISTSVAGL
INGSFEIGNL FFIVFVSYFG TKLHRPVVIG IGCVIMGLGC LLMSLPHFFM GRYEYETTIS
PTGNLSSNSF LCMENRTQTL KPTQDPAECV KEMKSLMWIC VMVGNIIRGI GETPIVPLGI
SYIEDFAKSE NSPLYIGILE MGKVAGPIFG LLLGSYCAQI YVDIGSVNTD DLTITPSDTR
WVGAWWIGFL VCAGVNILTS IPFFFLPKAL PKKGQQENVA VTKDGKVEKY GGQAREENLG
ITKDFLTFMK RLFCNPIYML FILTSVLQVN GFINKFTFLP KYLEQQYGKS TAEAIFLIGV
YSLPPICLGY LIGGFIMKKF KITVKKAAYL AFCLSVFEYL LFLCHFMLTC DNAAVAGLTT
SYKGVQHQLH VESKVLADCN TRCSCSTNTW DPVCGDNGVA YMSACLAGCK KFVGTGTNMV
FQDCSCIQSL GNSSAVLGLC KKGPECANRL QYFLILTIII SFIYSLTAIP GYMVFLRCVK
SEEKSLGVGL HTFCIRVFAG IPAPVYFGAL IDRTCLHWGT LKCGQRGACR MYDINSFRHI
YLGLPIALRG SSYLPAFFIL ILMRKFQFPG DIDSSATDHT EMMLGEKESE HTDVHGSPQV
ENDGELKTKL
