SO1A3_RAT
ID SO1A3_RAT Reviewed; 670 AA.
AC P70502; Q8R008; Q8R4J0; Q8R4J1; Q8R4J2; Q8R4J3; Q8R4J4; Q8R4J5; Q8R4J6;
AC Q8R4J7; Q8R4J8; Q8R4J9; Q8R4K0; Q8R4K1; Q9WTM0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Solute carrier organic anion transporter family member 1A3;
DE AltName: Full=OAT-K2;
DE AltName: Full=Sodium-independent organic anion transporter K1;
DE Short=OAT-K1;
DE AltName: Full=Solute carrier family 21 member 4;
DE AltName: Full=rOAT-K;
GN Name=Slco1a3; Synonyms=Oatp1a3, Slc21a4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=Sprague-Dawley; TISSUE=Kidney;
RX PubMed=8702823; DOI=10.1074/jbc.271.34.20719;
RA Saito H., Masuda S., Inui K.;
RT "Cloning and functional characterization of a novel rat organic anion
RT transporter mediating basolateral uptake of methotrexate in the kidney.";
RL J. Biol. Chem. 271:20719-20725(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 13).
RC TISSUE=Kidney;
RX PubMed=10101033;
RA Masuda S., Ibaramoto K., Takeuchi A., Saito H., Hashimoto Y., Inui K.;
RT "Cloning and functional characterization of a new multispecific organic
RT anion transporter, OAT-K2, in rat kidney.";
RL Mol. Pharmacol. 55:743-752(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3; 4; 5; 6; 7; 8; 9; 10; 11; 12; 14
RP AND 15).
RC STRAIN=Sprague-Dawley; TISSUE=Kidney;
RX PubMed=12180133; DOI=10.1080/10425170290019856;
RA Wolff M.W., Su T.-Z.;
RT "Cloning and tissue-specific expression of spliced variants of the rat
RT organic anion transporter (rOAT-K).";
RL DNA Seq. 13:15-25(2002).
CC -!- FUNCTION: Mediates the Na(+)-independent transport of organic anions
CC such as methotrexate, taurocholate, folate and prostaglandin E2. May
CC contribute to renal secretion and/or reabsorption of hydrophobic
CC anionic compounds. Mediates renal clearance of methotrexate from the
CC blood.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=15;
CC Name=1;
CC IsoId=P70502-1; Sequence=Displayed;
CC Name=2; Synonyms=K1;
CC IsoId=P70502-2; Sequence=VSP_006143, VSP_006145;
CC Name=3; Synonyms=K5;
CC IsoId=P70502-3; Sequence=VSP_006133, VSP_006143, VSP_006145;
CC Name=4; Synonyms=K6;
CC IsoId=P70502-4; Sequence=VSP_006143, VSP_006144;
CC Name=5; Synonyms=K14;
CC IsoId=P70502-5; Sequence=VSP_006134, VSP_006140, VSP_006143,
CC VSP_006145;
CC Name=6; Synonyms=K3;
CC IsoId=P70502-6; Sequence=VSP_006133, VSP_006143, VSP_006144;
CC Name=7; Synonyms=K8;
CC IsoId=P70502-7; Sequence=VSP_006135, VSP_006141, VSP_006143,
CC VSP_006145;
CC Name=8; Synonyms=K2;
CC IsoId=P70502-8; Sequence=VSP_006136, VSP_006143, VSP_006145;
CC Name=9; Synonyms=K4, K13;
CC IsoId=P70502-9; Sequence=VSP_006139, VSP_006146;
CC Name=10; Synonyms=K12;
CC IsoId=P70502-10; Sequence=VSP_006137, VSP_006143, VSP_006144;
CC Name=11; Synonyms=K7;
CC IsoId=P70502-11; Sequence=VSP_006136, VSP_006143, VSP_006144;
CC Name=12; Synonyms=K11;
CC IsoId=P70502-12; Sequence=VSP_006135, VSP_006141, VSP_006143,
CC VSP_006144;
CC Name=13; Synonyms=K2;
CC IsoId=P70502-13; Sequence=VSP_006138, VSP_006142, VSP_006143,
CC VSP_006145;
CC Name=14; Synonyms=K9;
CC IsoId=P70502-14; Sequence=VSP_006137, VSP_006143, VSP_006145;
CC Name=15; Synonyms=K10;
CC IsoId=P70502-15; Sequence=VSP_006134, VSP_006140, VSP_006143,
CC VSP_006144;
CC -!- TISSUE SPECIFICITY: All isoforms are detected in kidney, and many are
CC kidney specific. Isoforms 2 and 13 are also detected in liver. Isoforms
CC 4 and 9/K4 are ubiquitous, but isoform 9/K13 is kidney specific.
CC Isoforms 5 and 14 are detected in all tissues tested, with the
CC exception of pancreas and spleen. Isoforms 11 and 15 are detected in
CC kidney, pancreas and testis. Isoform 7 is detected in kidney, liver,
CC testis and spleen.
CC -!- SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family.
CC {ECO:0000305}.
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DR EMBL; D79981; BAA11476.1; -; mRNA.
DR EMBL; AB012662; BAA77793.1; -; mRNA.
DR EMBL; AF445993; AAL84221.1; -; mRNA.
DR EMBL; AF445994; AAL84222.1; -; mRNA.
DR EMBL; AF445995; AAL84223.1; -; mRNA.
DR EMBL; AF445996; AAL84224.1; -; mRNA.
DR EMBL; AF445997; AAL84225.1; -; mRNA.
DR EMBL; AF445998; AAL84226.1; -; mRNA.
DR EMBL; AF445999; AAL84227.1; -; mRNA.
DR EMBL; AF446000; AAL84228.1; -; mRNA.
DR EMBL; AF446001; AAL84229.1; -; mRNA.
DR EMBL; AF446002; AAL84230.1; -; mRNA.
DR EMBL; AF446003; AAL84231.1; -; mRNA.
DR EMBL; AF446004; AAL84232.1; -; mRNA.
DR EMBL; AF446005; AAL84233.1; -; mRNA.
DR EMBL; AF446006; AAL84234.1; -; mRNA.
DR RefSeq; NP_110464.1; NM_030837.1. [P70502-2]
DR AlphaFoldDB; P70502; -.
DR SMR; P70502; -.
DR STRING; 10116.ENSRNOP00000015112; -.
DR BindingDB; P70502; -.
DR ChEMBL; CHEMBL2073707; -.
DR TCDB; 2.A.60.1.4; the organo anion transporter (oat) family.
DR GlyGen; P70502; 4 sites.
DR iPTMnet; P70502; -.
DR PhosphoSitePlus; P70502; -.
DR PaxDb; P70502; -.
DR Ensembl; ENSRNOT00000000026; ENSRNOP00000000026; ENSRNOG00000010388. [P70502-2]
DR GeneID; 80899; -.
DR KEGG; rno:80899; -.
DR CTD; 80899; -.
DR RGD; 621387; Slc21a4.
DR VEuPathDB; HostDB:ENSRNOG00000030894; -.
DR VEuPathDB; HostDB:ENSRNOG00000047493; -.
DR eggNOG; KOG3626; Eukaryota.
DR GeneTree; ENSGT01050000244856; -.
DR HOGENOM; CLU_729505_0_0_1; -.
DR InParanoid; P70502; -.
DR OrthoDB; 1029129at2759; -.
DR PhylomeDB; P70502; -.
DR TreeFam; TF317540; -.
DR PRO; PR:P70502; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000010388; Expressed in adult mammalian kidney and 8 other tissues.
DR ExpressionAtlas; P70502; baseline and differential.
DR Genevisible; P70502; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IDA:RGD.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0098656; P:anion transmembrane transport; IDA:RGD.
DR GO; GO:0015721; P:bile acid and bile salt transport; IBA:GO_Central.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004156; OATP.
DR PANTHER; PTHR11388; PTHR11388; 1.
DR Pfam; PF07648; Kazal_2; 1.
DR Pfam; PF03137; OATP; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00805; oat; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..670
FT /note="Solute carrier organic anion transporter family
FT member 1A3"
FT /id="PRO_0000191045"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..40
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..59
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..155
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..184
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..242
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..267
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..333
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..353
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..377
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..405
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 406..513
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 514..536
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 537..545
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 546..571
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 572..605
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..623
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 624..670
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 433..488
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT REGION 277..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..296
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 483
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 492
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 439..469
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 445..465
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 454..486
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT VAR_SEQ 1..318
FT /note="Missing (in isoform 9)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006139"
FT VAR_SEQ 1..172
FT /note="Missing (in isoform 13)"
FT /evidence="ECO:0000303|PubMed:10101033"
FT /id="VSP_006138"
FT VAR_SEQ 1..156
FT /note="Missing (in isoform 10 and isoform 14)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006137"
FT VAR_SEQ 1..132
FT /note="Missing (in isoform 8 and isoform 11)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006136"
FT VAR_SEQ 1..102
FT /note="Missing (in isoform 7 and isoform 12)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006135"
FT VAR_SEQ 1..61
FT /note="Missing (in isoform 5 and isoform 15)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006134"
FT VAR_SEQ 1..23
FT /note="Missing (in isoform 3 and isoform 6)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006133"
FT VAR_SEQ 62..67
FT /note="NGSFEI -> MLCQDQ (in isoform 5 and isoform 15)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006140"
FT VAR_SEQ 103..111
FT /note="ISLPHFLMG -> MGPDALPRS (in isoform 7 and isoform
FT 12)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006141"
FT VAR_SEQ 173
FT /note="T -> M (in isoform 13)"
FT /evidence="ECO:0000303|PubMed:10101033"
FT /id="VSP_006142"
FT VAR_SEQ 289..298
FT /note="STEKRPRKKN -> KHREKAKEEK (in isoform 2, isoform 3,
FT isoform 4, isoform 5, isoform 6, isoform 7, isoform 8,
FT isoform 10, isoform 11, isoform 12, isoform 13, isoform 14
FT and isoform 15)"
FT /evidence="ECO:0000303|PubMed:10101033,
FT ECO:0000303|PubMed:12180133"
FT /id="VSP_006143"
FT VAR_SEQ 304..358
FT /note="Missing (in isoform 4, isoform 6, isoform 10,
FT isoform 11, isoform 12 and isoform 15)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006144"
FT VAR_SEQ 312..325
FT /note="PVLQPDLHAVHPYK -> LSCNPIYMLFTLIS (in isoform 2,
FT isoform 3, isoform 5, isoform 7, isoform 8, isoform 13 and
FT isoform 14)"
FT /evidence="ECO:0000303|PubMed:10101033,
FT ECO:0000303|PubMed:12180133"
FT /id="VSP_006145"
FT VAR_SEQ 319..325
FT /note="HAVHPYK -> MLFTLIS (in isoform 9)"
FT /evidence="ECO:0000303|PubMed:12180133"
FT /id="VSP_006146"
FT CONFLICT 391
FT /note="A -> R (in Ref. 1; BAA11476)"
FT /evidence="ECO:0000305"
FT CONFLICT 588..589
FT /note="AC -> R (in Ref. 1; BAA11476)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 670 AA; 73817 MW; E05F0B34A4C0ED9F CRC64;
MGDLEKGAAT HGAGCFAKIK VFLMALTCAY VSKSLSGTFM SSMLTQIERQ FGIPTAIVGF
INGSFEIGNL LLIIFVSYFG MKLHRPIVIG VGCAVMGLGC FIISLPHFLM GRYEYETTIL
PTSNLSSNSF LCMENQTQTL NPAQDPAECV KEVKSLMWIY VLVGNIIRGI GETPIMPLGV
SYIENFAKSE NSPLYIGILE TGKMIGPIFG LLLGSFCASI YVDTGSVNTD DLTITPTDIR
WVGAWWIGFL VCAGVNILIS IPFFFFPKTL PKEGLQENVD GTENAKEEST EKRPRKKNRG
ITKDFFPFLK SPVLQPDLHA VHPYKVLQVN AFNIYFSFLP KYLENQYGKS TAEVIFLMGV
YNLPAICIGY LIAGFMMKKF KITVKTAAFL AFCLSLSEYS FGFCNFLITC DNVPVAGLTN
SYERDQKPLY LENNVLADCN TRCSCLTKTW DPVCGDNGLA YMSACLAGCE KSVGTGTNMV
FHNCSCIQSP GNSSAVLGLC NKGPECTNKL QYLLILSGFL SILYSFAAIP GYMVFLRCIK
SEEKSLGIGI HAFCIRVFAG IPAPIYFGAL IDRTCLHWGT QKCGAPGACR MYDINSFRRI
YLGMSAALRG SSYLPAFVIV ILTRKFSLPG KINSSEMEIA EMKLTEKESQ CTDVHRNPKF
KNDGELKTKL
