SO1A4_RAT
ID SO1A4_RAT Reviewed; 661 AA.
AC O35913; O55224;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Solute carrier organic anion transporter family member 1A4;
DE AltName: Full=Brain digoxin carrier protein;
DE AltName: Full=Brain-specific organic anion transporter;
DE AltName: Full=OATP-B1;
DE AltName: Full=Sodium-independent organic anion-transporting polypeptide 2;
DE AltName: Full=Solute carrier family 21 member 5;
GN Name=Slco1a4; Synonyms=Oatp1a4, Oatp2, Slc21a5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Brain;
RX PubMed=9294213; DOI=10.1073/pnas.94.19.10346;
RA Noe B., Hagenbuch B., Stieger B., Meier P.J.;
RT "Isolation of a multispecific organic anion and cardiac glycoside
RT transporter from rat brain.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:10346-10350(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RX PubMed=9712861; DOI=10.1074/jbc.273.35.22395;
RA Abe T., Kakyo M., Sakagami H., Tokui T., Nishio T., Tanemoto M., Nomura H.,
RA Hebert S.C., Matsuno S., Kondo H., Yawo H.;
RT "Molecular characterization and tissue distribution of a new organic anion
RT transporter subtype (oatp3) that transports thyroid hormones and
RT taurocholate and comparison with oatp2.";
RL J. Biol. Chem. 273:22395-22401(1998).
CC -!- FUNCTION: Mediates the Na(+)-independent transport of organic anions
CC such as taurocholate, cholate, 17-beta-glucuronosyl estradiol, estrone-
CC 3-sulfate, the cardiac glycosides ouabain and digoxin and thyroid
CC hormones. May play an especially important role in the brain
CC accumulation and toxicity of digoxin and in the hepatobiliary and renal
CC excretion of cardiac glycosides.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highly expressed in brain, liver, and kidney but
CC not expressed in heart, spleen, lung, skeletal muscle, and testis.
CC -!- SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family.
CC {ECO:0000305}.
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DR EMBL; U88036; AAB80699.1; -; mRNA.
DR EMBL; U95011; AAC32669.1; -; mRNA.
DR RefSeq; NP_571981.1; NM_131906.1.
DR RefSeq; XP_017447901.1; XM_017592412.1.
DR AlphaFoldDB; O35913; -.
DR SMR; O35913; -.
DR STRING; 10116.ENSRNOP00000040237; -.
DR BindingDB; O35913; -.
DR ChEMBL; CHEMBL1781860; -.
DR GlyGen; O35913; 5 sites.
DR PaxDb; O35913; -.
DR PRIDE; O35913; -.
DR Ensembl; ENSRNOT00000043374; ENSRNOP00000042595; ENSRNOG00000047493.
DR GeneID; 170698; -.
DR KEGG; rno:170698; -.
DR CTD; 28250; -.
DR RGD; 621389; Slco1a4.
DR eggNOG; KOG3626; Eukaryota.
DR GeneTree; ENSGT01050000244856; -.
DR HOGENOM; CLU_008954_4_0_1; -.
DR InParanoid; O35913; -.
DR OMA; HFLMDRX; -.
DR OrthoDB; 1029129at2759; -.
DR PhylomeDB; O35913; -.
DR Reactome; R-RNO-159418; Recycling of bile acids and salts.
DR Reactome; R-RNO-879518; Transport of organic anions.
DR PRO; PR:O35913; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000047493; Expressed in liver and 14 other tissues.
DR Genevisible; O35913; RN.
DR GO; GO:0009925; C:basal plasma membrane; IDA:ARUK-UCL.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0015125; F:bile acid transmembrane transporter activity; IDA:RGD.
DR GO; GO:0008514; F:organic anion transmembrane transporter activity; IDA:RGD.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; ISO:RGD.
DR GO; GO:0015721; P:bile acid and bile salt transport; IEP:RGD.
DR GO; GO:0038183; P:bile acid signaling pathway; IEP:RGD.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0120188; P:regulation of bile acid secretion; IEP:RGD.
DR GO; GO:0010468; P:regulation of gene expression; IEP:RGD.
DR GO; GO:1904486; P:response to 17alpha-ethynylestradiol; IEP:RGD.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IDA:RGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004156; OATP.
DR PANTHER; PTHR11388; PTHR11388; 1.
DR Pfam; PF07648; Kazal_2; 1.
DR Pfam; PF03137; OATP; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00805; oat; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion transport; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..661
FT /note="Solute carrier organic anion transporter family
FT member 1A4"
FT /id="PRO_0000191047"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..40
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..59
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..154
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..183
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..202
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..241
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..266
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..310
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..332
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 333..352
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..376
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..380
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..404
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 405..512
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 513..535
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 536..544
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 545..570
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 571..604
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 605..622
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 623..661
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 432..487
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT MOD_RES 633
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P46720"
FT MOD_RES 634
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P46720"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 443
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 482
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 491
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 438..468
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 444..464
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 453..485
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT CONFLICT 360
FT /note="Y -> N (in Ref. 2; AAC32669)"
FT /evidence="ECO:0000305"
FT CONFLICT 617
FT /note="F -> FF (in Ref. 2; AAC32669)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 661 AA; 73252 MW; 692DA69FC85299D2 CRC64;
MGKSEKRVAT HGVRCFAKIK MFLLALTCAY VSKSLSGTYM NSMLTQIERQ FGIPTSIVGL
INGSFEIGNL LLIIFVSYFG TKLHRPIMIG VGCAVMGLGC FLISLPHFLM GQYEYETILP
TSNVSSNSFF CVENRSQTLN PTQDPSECVK EMKSLMWIYV LVGNIIRGIG ETPIMPLGIS
YIEDFAKSEN SPLYIGILET GMTIGPLIGL LLASSCANIY VDIESVNTDD LTITPTDTRW
VGAWWIGFLV CAGVNILTSF PFFFFPKTLP KEGLQENVDG TENAKEKKHR KKAKEEKRGI
TKDFFVFMKS LSCNPIYMLF ILISVLQFNA FINSFTFMPK YLEQQYGKST AEVVFLMGLY
MLPPICLGYL IGGLIMKKFK VTVKKAAHLA FWLCLSEYLL SFLSYVMTCD NFPVAGLTTS
YEGVQHQLYV ENKVLADCNT RCNCSTNTWD PVCGDNGLAY MSACLAGCEK SVGTGTNMVF
QNCSCIQSSG NSSAVLGLCN KGPDCANKLQ YFLIIAIFGC FIYSLAGIPG YMVLLRCIKS
EEKSLGVGLH AFCIRILAGI PAPIYFGALI DRTCLHWGTL KCGEPGACRM YDINSFRRLY
LGLPAALRGA SFVPAFFILR LTRTFQFPGD IESSKTDHAE MKLTLKESEC TEVLRSKVTE
D
