SO2A1_MOUSE
ID SO2A1_MOUSE Reviewed; 643 AA.
AC Q9EPT5;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Solute carrier organic anion transporter family member 2A1 {ECO:0000305};
DE AltName: Full=Prostaglandin transporter;
DE Short=PGT;
DE AltName: Full=Solute carrier family 21 member 2;
GN Name=Slco2a1 {ECO:0000312|MGI:MGI:1346021}; Synonyms=Oatp2a1, Slc21a2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND MUTAGENESIS OF VAL-610 AND
RP ILE-611.
RC TISSUE=Lung;
RX PubMed=10484490; DOI=10.1152/ajpregu.1999.277.3.r734;
RA Pucci M.L., Bao Y., Chan B., Itoh S., Lu R., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Schuster V.L.;
RT "Cloning of mouse prostaglandin transporter PGT cDNA: species-specific
RT substrate affinities.";
RL Am. J. Physiol. 277:R734-R741(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary cancer;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transports PGD2, as well as PGE1, PGE2 and PGF2A. Mediates
CC the clearance of prostaglandins from the circulation through uptake
CC across cell membrane which allows cytoplasmic oxidation and
CC prostaglandin signal termination. May mediate the release of newly
CC synthesized prostaglandins from cells and the transepithelial transport
CC of prostaglandins. {ECO:0000250|UniProtKB:Q92959}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin D2(out) = prostaglandin D2(in);
CC Xref=Rhea:RHEA:50976, ChEBI:CHEBI:57406;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin E1(out) = prostaglandin E1(in);
CC Xref=Rhea:RHEA:50980, ChEBI:CHEBI:57397;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin E2(out) = prostaglandin E2(in);
CC Xref=Rhea:RHEA:50984, ChEBI:CHEBI:606564;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin F2alpha(out) = prostaglandin F2alpha(in);
CC Xref=Rhea:RHEA:50988, ChEBI:CHEBI:57404;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=thromboxane B2(out) = thromboxane B2(in);
CC Xref=Rhea:RHEA:50992, ChEBI:CHEBI:90696;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q92959};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9EPT5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9EPT5-2; Sequence=VSP_006128, VSP_006129;
CC -!- TISSUE SPECIFICITY: Highly expressed in lung and liver. Detected at
CC lower levels in kidney and skeletal muscle.
CC -!- SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family.
CC {ECO:0000305}.
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DR EMBL; AF323958; AAG40332.1; -; mRNA.
DR EMBL; BC035200; AAH35200.1; -; mRNA.
DR CCDS; CCDS23448.1; -. [Q9EPT5-1]
DR RefSeq; NP_201571.2; NM_033314.3. [Q9EPT5-1]
DR AlphaFoldDB; Q9EPT5; -.
DR SMR; Q9EPT5; -.
DR STRING; 10090.ENSMUSP00000035148; -.
DR BindingDB; Q9EPT5; -.
DR ChEMBL; CHEMBL2073699; -.
DR GlyGen; Q9EPT5; 3 sites.
DR iPTMnet; Q9EPT5; -.
DR PhosphoSitePlus; Q9EPT5; -.
DR SwissPalm; Q9EPT5; -.
DR MaxQB; Q9EPT5; -.
DR PaxDb; Q9EPT5; -.
DR PeptideAtlas; Q9EPT5; -.
DR PRIDE; Q9EPT5; -.
DR ProteomicsDB; 261103; -. [Q9EPT5-1]
DR ProteomicsDB; 261104; -. [Q9EPT5-2]
DR Antibodypedia; 33384; 75 antibodies from 16 providers.
DR DNASU; 24059; -.
DR Ensembl; ENSMUST00000035148; ENSMUSP00000035148; ENSMUSG00000032548. [Q9EPT5-1]
DR Ensembl; ENSMUST00000188664; ENSMUSP00000140533; ENSMUSG00000032548. [Q9EPT5-2]
DR GeneID; 24059; -.
DR KEGG; mmu:24059; -.
DR UCSC; uc009rgd.1; mouse. [Q9EPT5-1]
DR CTD; 6578; -.
DR MGI; MGI:1346021; Slco2a1.
DR VEuPathDB; HostDB:ENSMUSG00000032548; -.
DR eggNOG; KOG3626; Eukaryota.
DR GeneTree; ENSGT01050000244856; -.
DR HOGENOM; CLU_008954_4_1_1; -.
DR InParanoid; Q9EPT5; -.
DR OMA; ISWKVKR; -.
DR PhylomeDB; Q9EPT5; -.
DR TreeFam; TF317540; -.
DR Reactome; R-MMU-879518; Transport of organic anions.
DR BioGRID-ORCS; 24059; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Slco2a1; mouse.
DR PRO; PR:Q9EPT5; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9EPT5; protein.
DR Bgee; ENSMUSG00000032548; Expressed in placenta labyrinth and 179 other tissues.
DR ExpressionAtlas; Q9EPT5; baseline and differential.
DR Genevisible; Q9EPT5; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015132; F:prostaglandin transmembrane transporter activity; IDA:MGI.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015732; P:prostaglandin transport; IDA:MGI.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004156; OATP.
DR PANTHER; PTHR11388; PTHR11388; 1.
DR Pfam; PF07648; Kazal_2; 1.
DR Pfam; PF03137; OATP; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00805; oat; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Lipid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..643
FT /note="Solute carrier organic anion transporter family
FT member 2A1"
FT /id="PRO_0000191059"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..52
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..71
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..98
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..123
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..167
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..196
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..254
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..279
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..320
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..342
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..362
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..386
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 387..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..414
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..517
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 518..540
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 541..549
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 550..575
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 576..609
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 610..628
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 629..643
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 437..495
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 477
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 490
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 443..473
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 449..469
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 458..493
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT VAR_SEQ 368..414
FT /note="GAVNLPAAALGMLFGGILMKRFVFPLQTIPRVAATIMTISIILCAPL -> A
FT HQVLYIRSLPPAAGTACAQIPSSTLSAETMESSTSPPAMLAAAAST (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_006128"
FT VAR_SEQ 415..643
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_006129"
FT MUTAGEN 610
FT /note="V->M: No effect."
FT /evidence="ECO:0000269|PubMed:10484490"
FT MUTAGEN 611
FT /note="I->G: No effect."
FT /evidence="ECO:0000269|PubMed:10484490"
FT CONFLICT 3
FT /note="L -> F (in Ref. 1; AAG40332)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 643 AA; 70147 MW; AC487386F847D9D8 CRC64;
MGLLPKPGAR QGSGTSSVPA RRCSRSVFNN IKVFVLCHGL LQLCQLLYSA YFKSSLTTIE
KRFGLSSSSS GLISSLNEIS NAILIIFVSY FGSRVNRPRM IGIGGLLLAA GAFVLTLPHF
LSEPYQYAST TAGNSSHFQT DLCQKHLPGL LPSKCHSTVP DTQKETSSMW SLMVVAQLLA
GVGTVPIQPF GISYVDDFAE PTNSPLYISI LFAIAVFGPA FGYLLGSVML RIFVDYGRVD
TATVNLSPGD PRWIGAWWLG LLISSGFLIV TSLPFFFFPR AMSRGAERSV IAEETMKMEE
DKSRGSLMDF IKRFPRIFLR LLMNPLFMLV VLSQCTFSSV IAGLSTFLNK FLEKQYDASA
AYANLLIGAV NLPAAALGML FGGILMKRFV FPLQTIPRVA ATIMTISIIL CAPLFFMGCS
TPAVAEVYPP STPSSIHPQP PACRRDCLCP DSVFHPVCGD NGVEYLSPCH AGCSSLNVSS
AASKQPIYLN CSCVTGGSAS AKTGSCPTSC AQLLLPSIFL ISFVALIACV SHNPLYMMVL
RVVNQDEKSF AIGVQFLLMR LLAWLPSPSL YGLLIDSSCI RWNYLCSGRR GACAYYDNDA
LRNRYLGLQV IYKVLGTLLL FFISWRVKKN REYSLQENAS GLI
