SO2A1_RAT
ID SO2A1_RAT Reviewed; 644 AA.
AC Q00910;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Solute carrier organic anion transporter family member 2A1 {ECO:0000305};
DE AltName: Full=Matrin F/G 1;
DE AltName: Full=Prostaglandin transporter;
DE Short=PGT {ECO:0000303|PubMed:7754369};
DE AltName: Full=Solute carrier family 21 member 2;
GN Name=Slco2a1 {ECO:0000312|RGD:3051}; Synonyms=Matr1, Slc21a2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=2068100; DOI=10.1073/pnas.88.14.6186;
RA Hakes D.J., Berezney R.;
RT "Molecular cloning of matrin F/G: a DNA binding protein of the nuclear
RT matrix that contains putative zinc finger motifs.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:6186-6190(1991).
RN [2]
RP CHARACTERIZATION, AND TRANSPORTER ACTIVITY.
RX PubMed=7754369; DOI=10.1126/science.7754369;
RA Kanai N., Lu R., Satriano J.A., Bao Y., Wolkoff A.W., Schuster V.L.;
RT "Identification and characterization of a prostaglandin transporter.";
RL Science 268:866-869(1995).
RN [3]
RP FUNCTION, AND TRANSPORTER ACTIVITY.
RX PubMed=15044627; DOI=10.1124/mol.65.4.973;
RA Nomura T., Lu R., Pucci M.L., Schuster V.L.;
RT "The two-step model of prostaglandin signal termination: in vitro
RT reconstitution with the prostaglandin transporter and prostaglandin 15
RT dehydrogenase.";
RL Mol. Pharmacol. 65:973-978(2004).
CC -!- FUNCTION: Transports PGD2, as well as PGE1, PGE2 and PGF2A. Mediates
CC the clearance of prostaglandins from the circulation through uptake
CC across cell membrane which allows cytoplasmic oxidation and
CC prostaglandin signal termination (PubMed:15044627, PubMed:7754369). May
CC mediate the release of newly synthesized prostaglandins from cells and
CC the transepithelial transport of prostaglandins (Probable).
CC {ECO:0000269|PubMed:15044627, ECO:0000269|PubMed:7754369, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin E2(out) = prostaglandin E2(in);
CC Xref=Rhea:RHEA:50984, ChEBI:CHEBI:606564;
CC Evidence={ECO:0000269|PubMed:15044627, ECO:0000269|PubMed:7754369};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin D2(out) = prostaglandin D2(in);
CC Xref=Rhea:RHEA:50976, ChEBI:CHEBI:57406;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin E1(out) = prostaglandin E1(in);
CC Xref=Rhea:RHEA:50980, ChEBI:CHEBI:57397;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=prostaglandin F2alpha(out) = prostaglandin F2alpha(in);
CC Xref=Rhea:RHEA:50988, ChEBI:CHEBI:57404;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=thromboxane B2(out) = thromboxane B2(in);
CC Xref=Rhea:RHEA:50992, ChEBI:CHEBI:90696;
CC Evidence={ECO:0000250|UniProtKB:Q92959};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:7754369};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family.
CC {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be a nuclear DNA-binding protein.
CC {ECO:0000305|PubMed:2068100}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA41574.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAA41574.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M64862; AAA41574.1; ALT_SEQ; mRNA.
DR PIR; A41120; A41120.
DR AlphaFoldDB; Q00910; -.
DR SMR; Q00910; -.
DR STRING; 10116.ENSRNOP00000012162; -.
DR BindingDB; Q00910; -.
DR ChEMBL; CHEMBL2073680; -.
DR GuidetoPHARMACOLOGY; 1223; -.
DR SwissLipids; SLP:000001648; -.
DR TCDB; 2.A.60.1.2; the organo anion transporter (oat) family.
DR GlyGen; Q00910; 4 sites.
DR PaxDb; Q00910; -.
DR PeptideAtlas; Q00910; -.
DR PRIDE; Q00910; -.
DR RGD; 3051; Slco2a1.
DR eggNOG; KOG3626; Eukaryota.
DR InParanoid; Q00910; -.
DR Reactome; R-RNO-879518; Transport of organic anions.
DR PRO; PR:Q00910; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015132; F:prostaglandin transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015732; P:prostaglandin transport; ISO:RGD.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004156; OATP.
DR PANTHER; PTHR11388; PTHR11388; 1.
DR Pfam; PF07648; Kazal_2; 1.
DR Pfam; PF03137; OATP; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00805; oat; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; Lipid transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..644
FT /note="Solute carrier organic anion transporter family
FT member 2A1"
FT /id="PRO_0000191060"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 551..571
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..626
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 438..496
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 478
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 491
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 639
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 444..474
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 450..470
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 459..494
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ SEQUENCE 644 AA; 70387 MW; 143841277CC95F85 CRC64;
MGLLLKPGAR QGSGTSSVPD RRCPRSVFSN IKVFVLCHGL LQLCQLLYSA YFKSSLTTIE
KRFGLSSSSS GLISSLNEIS NATLIIFISY FGSRVNRPRM IGIGGLLLAA GAFVLTLPHF
LSEPYQYTST TDGNRSSFQT DLCQKHFGAL PPSKCHSTVP DTHKETSSLW GLMVVAQLLA
GIGTVPIQPF GISYVDDFAE PTNSPLYISI LFAIAVFGPA FGYLLGSVML RIFVDYGRVD
TATVNLSPGD PRWIGAWWLG LLISSGFLIV TSLPFFFFPR AMSRGAERSV TAEETMQTEE
DKSRGSLMDF IKRFPRIFLR LLMNPLFMLV VLSQCTFSSV IAGLSTFLNK FLEKQYGATA
AYANFLIGAV NLPAAALGML FGGILMKRFV FPLQTIPRVA ATIITISMIL CVPLFFMGCS
TSAVAEVYPP STSSSIHPQQ PPACRRDCSC PDSFFHPVCG DNGVEYVSPC HAGCSSTNTS
SEASKEPIYL NCSCVSGGSA SAKTGSCPTS CAQLLLPSIF LISFAALIAC ISHNPLYMMV
LRVVNQDEKS FAIGVQFLLM RLLAWLPAPS LYGLLIDSSC VRWNYLCSGR RGACAYYDND
ALRNRYLGLQ MVYKALGTLL LFFISWRMKK NREYSLQENT SGLI
