SO74D_DROME
ID SO74D_DROME Reviewed; 819 AA.
AC Q9VVH9;
DT 10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Solute carrier organic anion transporter family member 74D {ECO:0000305};
DE AltName: Full=Ecdysone Importer {ECO:0000303|PubMed:30293839};
DE Short=EcI {ECO:0000303|PubMed:30293839};
DE AltName: Full=Organic anion transporting polypeptide 74D {ECO:0000312|FlyBase:FBgn0036732};
GN Name=Oatp74D {ECO:0000312|FlyBase:FBgn0036732};
GN ORFNames=CG7571 {ECO:0000312|FlyBase:FBgn0036732};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAN71191.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71191.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAN71191.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=30293839; DOI=10.1016/j.devcel.2018.09.012;
RA Okamoto N., Viswanatha R., Bittar R., Li Z., Haga-Yamanaka S., Perrimon N.,
RA Yamanaka N.;
RT "A Membrane Transporter Is Required for Steroid Hormone Uptake in
RT Drosophila.";
RL Dev. Cell 47:294-305(2018).
CC -!- FUNCTION: Transporter that mediates the cellular uptake of
CC ecdysteroids, including ecdysone, from the hemolymph.
CC {ECO:0000269|PubMed:30293839}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|RuleBase:RU362056,
CC ECO:0000269|PubMed:30293839}; Multi-pass membrane protein
CC {ECO:0000255|RuleBase:RU362056, ECO:0000269|PubMed:30293839}.
CC -!- DEVELOPMENTAL STAGE: Expressed ubiquitously during development in
CC central nervous system, imaginal disk, fat body, gut, and to a lesser
CC extent in Malpighian tubules. {ECO:0000269|PubMed:30293839}.
CC -!- DISRUPTION PHENOTYPE: Development is arrested at the end of the first
CC instar larval stage (PubMed:30293839). RNAi-mediated knockdown blocks
CC ecdysone-dependent fat body cell migration into the pupal head
CC (PubMed:30293839). In the fat body, results in defective ecdysone
CC signaling (PubMed:30293839). {ECO:0000269|PubMed:30293839}.
CC -!- SIMILARITY: Belongs to the organo anion transporter (TC 2.A.60) family.
CC {ECO:0000255|RuleBase:RU362056}.
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DR EMBL; AE014296; AAF49332.2; -; Genomic_DNA.
DR EMBL; AE014296; ACZ94731.1; -; Genomic_DNA.
DR EMBL; AE014296; AHN58120.1; -; Genomic_DNA.
DR EMBL; AE014296; AHN58121.1; -; Genomic_DNA.
DR EMBL; BT001436; AAN71191.1; -; mRNA.
DR RefSeq; NP_001163460.1; NM_001169989.2.
DR RefSeq; NP_001287095.1; NM_001300166.1.
DR RefSeq; NP_001287096.1; NM_001300167.1.
DR RefSeq; NP_648989.1; NM_140732.3.
DR AlphaFoldDB; Q9VVH9; -.
DR SMR; Q9VVH9; -.
DR IntAct; Q9VVH9; 3.
DR STRING; 7227.FBpp0074939; -.
DR GlyGen; Q9VVH9; 6 sites.
DR PaxDb; Q9VVH9; -.
DR PRIDE; Q9VVH9; -.
DR DNASU; 39954; -.
DR EnsemblMetazoa; FBtr0075173; FBpp0074939; FBgn0036732.
DR EnsemblMetazoa; FBtr0302041; FBpp0291251; FBgn0036732.
DR EnsemblMetazoa; FBtr0345117; FBpp0311342; FBgn0036732.
DR EnsemblMetazoa; FBtr0345118; FBpp0311343; FBgn0036732.
DR GeneID; 39954; -.
DR KEGG; dme:Dmel_CG7571; -.
DR UCSC; CG7571-RA; d. melanogaster.
DR CTD; 39954; -.
DR FlyBase; FBgn0036732; Oatp74D.
DR VEuPathDB; VectorBase:FBgn0036732; -.
DR eggNOG; KOG3626; Eukaryota.
DR GeneTree; ENSGT01050000244906; -.
DR HOGENOM; CLU_008954_1_3_1; -.
DR InParanoid; Q9VVH9; -.
DR OMA; CTHPKDK; -.
DR OrthoDB; 1029129at2759; -.
DR PhylomeDB; Q9VVH9; -.
DR BioGRID-ORCS; 39954; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 39954; -.
DR PRO; PR:Q9VVH9; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036732; Expressed in oviduct (Drosophila) and 27 other tissues.
DR ExpressionAtlas; Q9VVH9; baseline and differential.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:FlyBase.
DR GO; GO:0043229; C:intracellular organelle; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0022804; F:active transmembrane transporter activity; ISS:FlyBase.
DR GO; GO:0015347; F:sodium-independent organic anion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; ISS:FlyBase.
DR GO; GO:0035076; P:ecdysone receptor-mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0002164; P:larval development; IMP:UniProtKB.
DR GO; GO:0071702; P:organic substance transport; ISS:FlyBase.
DR GO; GO:0043252; P:sodium-independent organic anion transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004156; OATP.
DR PANTHER; PTHR11388; PTHR11388; 1.
DR Pfam; PF03137; OATP; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00805; oat; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..819
FT /note="Solute carrier organic anion transporter family
FT member 74D"
FT /id="PRO_0000446514"
FT TOPO_DOM 1..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 175..195
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..213
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 214..234
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 235..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 243..263
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..332
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 333..353
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 354..373
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 374..394
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..413
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 414..434
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 435..488
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 489..509
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 510..522
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 523..543
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 544..553
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 554..574
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 575..667
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 668..688
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 689..707
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 708..728
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 729..756
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 757..777
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 778..819
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 593..651
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT REGION 1..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..466
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 787..819
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..82
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 798..819
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 284
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 293
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 309
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 401
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 604
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 640
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 599..630
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 607..626
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 616..649
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ SEQUENCE 819 AA; 88506 MW; 2C33EAF513C0D87C CRC64;
MTKSNGDVEA AAQVQSLGGK PSNGHGQLNG NGYHQNGGRR DSSQAFTPLL SQHNNGTTNG
EVTTPPPSTV LYESTPSNNN EWKAPEDLGH LKNGLGNILS SNNNGTGNGH SLSEKYAHEQ
APLTGGYKLP PRSSESEESD FDSDLNGGSS AESSSSCGLF GCRPRWARRF ASTHVFMVVF
LLAYILQGMY MTYFVSVITT IEKLFQIKSK TTGILLSASE MGQICTAMLL TYFAGRGHRP
RWIACGMVLF SIAAFSCALP HFIFGEQLMH SSVILQQTQV SPPNNSFSSH WLNASSEQVN
PNLCILGGNQ THSGSECNEE RQLEQASHSK ITVIVLCIFF GSLLSSGIGQ TAVATLGIPY
IDDNVGSKQS PMYMAVTIGM RILGPASGFI FGSFCTRWYV NFSNPGFDAT DPRWIGAWWL
GPVAIGSLML LASIAMFSFP KQLRGKQKPP GQTATPAAPV EPEEKPKLKD FPKTVRRQLS
NDILMFRTAS CVFHLLPIAG LYTFLPKYLE TQFRLATYDA NMIAAFCGIL VMGIGIVISG
LFILKRKPTA RGVAAWIAFT ALVYSAGMII LMFIGCSMND FAGYKPSDGN SPALIEPTCS
AALNCTCDKE NFAPICADGK MYISACHAGC SSSSLRPSDN RTLYSDCACI PDAPEAVNGY
CDNNCKNFIY FILIFAICVF MHSTSEVGSM LLVMRCTHPK DKAMAMGVIQ SAIGLFGNVP
CPIIYGAVVD SACLIWKSVC GKHGACSLYD ADTFRQYFLG ITAGIMFLAF LMDLVVWRKA
HRIDIAPEDP QEGGPASNGR TLEVSESKQP ITPAPDTTV
