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SOAT2_CHLAE
ID   SOAT2_CHLAE             Reviewed;         526 AA.
AC   O77759;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Sterol O-acyltransferase 2 {ECO:0000305};
DE            EC=2.3.1.26 {ECO:0000250|UniProtKB:O75908};
DE   AltName: Full=Acyl-coenzyme A:cholesterol acyltransferase 2;
DE            Short=ACAT-2;
DE   AltName: Full=Cholesterol acyltransferase 2;
GN   Name=SOAT2; Synonyms=ACAT2;
OS   Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Chlorocebus.
OX   NCBI_TaxID=9534;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Liver;
RX   PubMed=9756918; DOI=10.1074/jbc.273.41.26747;
RA   Anderson R.A., Joyce C., Davis M., Reagan J.W., Clark M., Shelness G.S.,
RA   Rudel L.L.;
RT   "Identification of a form of acyl-CoA:cholesterol acyltransferase specific
RT   to liver and intestine in nonhuman primates.";
RL   J. Biol. Chem. 273:26747-26754(1998).
CC   -!- FUNCTION: Catalyzes the formation of fatty acid-cholesterol esters,
CC       which are less soluble in membranes than cholesterol. Plays a role in
CC       lipoprotein assembly and dietary cholesterol absorption. Utilizes
CC       oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-
CC       octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for
CC       lipoprotein secretion from hepatocytes and intestinal mucosa.
CC       {ECO:0000250|UniProtKB:O75908}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty acyl-CoA + a sterol = a sterol ester + CoA;
CC         Xref=Rhea:RHEA:59816, ChEBI:CHEBI:15889, ChEBI:CHEBI:35915,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:83139; EC=2.3.1.26;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:59817;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an acyl-CoA + cholesterol = a cholesterol ester + CoA;
CC         Xref=Rhea:RHEA:17729, ChEBI:CHEBI:16113, ChEBI:CHEBI:17002,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:58342;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17730;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z)-octadecenoyl-CoA + cholesterol = cholesteryl (9Z-
CC         octadecenoate) + CoA; Xref=Rhea:RHEA:41436, ChEBI:CHEBI:16113,
CC         ChEBI:CHEBI:46898, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41437;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + cholesterol =
CC         (5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-cholesterol + CoA;
CC         Xref=Rhea:RHEA:46612, ChEBI:CHEBI:16113, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:73862, ChEBI:CHEBI:84969;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46613;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(9Z,12Z,15Z)-octadecatrienoyl-CoA + cholesterol = (9Z,12Z,15Z-
CC         octadecatrienoyl)-cholesterol + CoA; Xref=Rhea:RHEA:46620,
CC         ChEBI:CHEBI:16113, ChEBI:CHEBI:57287, ChEBI:CHEBI:74034,
CC         ChEBI:CHEBI:84341; Evidence={ECO:0000250|UniProtKB:O75908};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46621;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + cholesterol =
CC         cholesteryl (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA;
CC         Xref=Rhea:RHEA:42816, ChEBI:CHEBI:16113, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57368, ChEBI:CHEBI:82751;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42817;
CC         Evidence={ECO:0000250|UniProtKB:O75908};
CC   -!- SUBUNIT: May form homo- or heterodimers (By similarity). Interacts with
CC       INSIG1; the interaction is direct and promotes association with
CC       AMFR/gp78 (By similarity). {ECO:0000250|UniProtKB:O75908,
CC       ECO:0000250|UniProtKB:P35610}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:O88908}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Liver and intestine. Expression is six-fold greater
CC       in hepatocytes than in kupffer cells. {ECO:0000269|PubMed:9756918}.
CC   -!- DOMAIN: Each protomer consists of 9 transmembrane segments, which
CC       enclose a cytosolic tunnel and a transmembrane tunnel that converge at
CC       the predicted catalytic site: acyl-CoA enters the active site through
CC       the cytosolic tunnel, whereas cholesterol enters from the side through
CC       the transmembrane tunnel. {ECO:0000250|UniProtKB:P35610}.
CC   -!- PTM: Polyubiquitinated by AMFR/gp78 at Cys-281, leading to its
CC       degradation when the lipid levels are low. Association with AMFR/gp78
CC       is mediated via interaction with INSIG1. High concentration of
CC       cholesterol and fatty acid results in Cys-281 oxidation, preventing
CC       ubiquitination at the same site, resulting in protein stabilization.
CC       {ECO:0000250|UniProtKB:O75908}.
CC   -!- PTM: Oxidized at Cys-281: high concentration of cholesterol and fatty
CC       acid induce reactive oxygen species, which oxidizes Cys-281, preventing
CC       ubiquitination at the same site, and resulting in protein
CC       stabilization. {ECO:0000250|UniProtKB:O75908}.
CC   -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC       Sterol o-acyltransferase subfamily. {ECO:0000305}.
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DR   EMBL; AF053234; AAC62929.1; -; mRNA.
DR   AlphaFoldDB; O77759; -.
DR   SMR; O77759; -.
DR   BindingDB; O77759; -.
DR   ChEMBL; CHEMBL3879854; -.
DR   BRENDA; 2.3.1.26; 175.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0034736; F:cholesterol O-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004772; F:sterol O-acyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0034435; P:cholesterol esterification; ISS:UniProtKB.
DR   GO; GO:0042632; P:cholesterol homeostasis; IEA:InterPro.
DR   GO; GO:0008203; P:cholesterol metabolic process; ISS:UniProtKB.
DR   InterPro; IPR004299; MBOAT_fam.
DR   InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR   InterPro; IPR030687; Sterol_acyltranf_meta.
DR   PANTHER; PTHR10408; PTHR10408; 1.
DR   Pfam; PF03062; MBOAT; 1.
DR   PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
DR   PIRSF; PIRSF500230; Sterol_acyltranf_ACAT; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Cholesterol metabolism; Endoplasmic reticulum;
KW   Lipid metabolism; Membrane; Oxidation; Steroid metabolism;
KW   Sterol metabolism; Thioester bond; Transferase; Transmembrane;
KW   Transmembrane helix; Ubl conjugation.
FT   CHAIN           1..526
FT                   /note="Sterol O-acyltransferase 2"
FT                   /id="PRO_0000207644"
FT   TOPO_DOM        1..120
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        121..142
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        143..162
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        163..188
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        189..200
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        201..224
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        225..232
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        233..256
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        257..297
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        298..330
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        331..347
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        348..373
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        374..421
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        422..446
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        447..452
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        453..468
FT                   /note="Helical; Name=8"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        469..474
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        475..506
FT                   /note="Helical; Name=9"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   TOPO_DOM        507..526
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          75..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           381..387
FT                   /note="FYXDWWN motif"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   COMPBIAS        13..37
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        438
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         119
FT                   /ligand="cholesterol"
FT                   /ligand_id="ChEBI:CHEBI:16113"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         393
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         396
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         399
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         403
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         411
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   BINDING         434
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT                   /evidence="ECO:0000250|UniProtKB:P35610"
FT   MOD_RES         281
FT                   /note="Cysteine sulfenic acid (-SOH); alternate"
FT                   /evidence="ECO:0000250|UniProtKB:O75908"
FT   CROSSLNK        281
FT                   /note="Glycyl cysteine thioester (Cys-Gly) (interchain with
FT                   G-Cter in ubiquitin); alternate"
FT                   /evidence="ECO:0000250|UniProtKB:O75908"
SQ   SEQUENCE   526 AA;  60412 MW;  FA5E29F48386DB43 CRC64;
     MEPGGARLRL QRTEGPGGER EHQPCRDGNT ETHRAPDLVK WTRHMEAVKA QLLEQAQGQL
     RELLDRAMWE AIQSYPSQDK PPPLPPPDSL SRTQEPSLGK QKVFIIRKSL LDELMEVQHF
     RTIYHMFIAG LCVFIISTLA IDFIDEGRLL LEFDLLIFSF GQLPLALVTW VPMFLSTLLA
     PYQALRLWAR PGARGTWTLG AGLGCALLAA HALVLCALPV HVAVEHQLPP ASRCVLVFEQ
     VRFLMKSYSF LREAVPGTLR ARRGEGIQAP SFSSYLYFLF CPTLIYRETY PRTPYIRWNY
     VAKNFAQALG CVLYACFILG RLCVPVFANM SREPFSTRAL VLSILHATLP GIFMLLLIFF
     AFLHCWLNAF AEMLRFGDRM FYRDWWNSTS FSNYYRTWNV VVHDWLYSYV YQDGLWLLGA
     QARGVAMLGV FLVSAVAHEY IFCFVLGFFY PVMLILFLVI GGMLNFMMHD QHTGPAWNVL
     MWTMLFLGQG IQVSLYCQEW YARRHCPLPQ TTFWGLVTPR SWSCHT
 
 
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