SOAT_BOVIN
ID SOAT_BOVIN Reviewed; 377 AA.
AC A6QP84; A2IB93; A4ZYB2; A4ZYB3; A4ZYB4; Q206K3;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 2.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Sodium-dependent organic anion transporter {ECO:0000303|Ref.1};
DE Short=Soat;
DE AltName: Full=Solute carrier family 10 member 6;
DE Short=SLC10A6 {ECO:0000303|Ref.1};
GN Name=SLC10A6; Synonyms=SOAT;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC TISSUE=Placenta;
RA Greven H., Geyer J., Schuler G., Hoffmann B.;
RT "Cloning of sodium-dependent organic anion transporter (SLC10A6) from
RT bovine placenta.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Hereford; TISSUE=Fetal muscle;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 120-237 (ISOFORM 1).
RC TISSUE=Testis;
RA Lekhkota O., Brehm R.H., Geyer J., Bergmann M.;
RT "Cloning of the sodium-dependent organic anion transporter (SOAT) from
RT bovine testis.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transports sulfoconjugated steroid hormones from the
CC extracellular compartment into the cytosol in a sodium-dependent manner
CC without hydrolysis. Steroid sulfate hormones are commonly considered to
CC be biologically inactive metabolites, that may be activated by steroid
CC sulfatases into free steroids (By similarity). May play an important
CC role by delivering sulfoconjugated steroids to specific target cells in
CC reproductive organs (By similarity). May play a role transporting the
CC estriol precursor 16alpha-hydroxydehydroepiandrosterone 3-sulfate (16a-
CC OH-DHEAS) at the fetal blood vessel endothelium (By similarity). Can
CC also transport other sulfoconjugated molecules such as taurolithocholic
CC acid-3-sulfate and sulfoconjugated pyrenes (By similarity).
CC {ECO:0000250|UniProtKB:Q3KNW5, ECO:0000250|UniProtKB:Q9CXB2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=estrone 3-sulfate(out) + 2 Na(+)(out) = estrone 3-sulfate(in)
CC + 2 Na(+)(in); Xref=Rhea:RHEA:71083, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:60050; Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-estradiol 3-sulfate(out) + 2 Na(+)(out) = 17beta-
CC estradiol 3-sulfate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71087,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:136582;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dehydroepiandrosterone 3-sulfate(out) + 2 Na(+)(out) =
CC dehydroepiandrosterone 3-sulfate(in) + 2 Na(+)(in);
CC Xref=Rhea:RHEA:71091, ChEBI:CHEBI:29101, ChEBI:CHEBI:57905;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=androst-5-ene-diol 3-sulfate(out) + 2 Na(+)(out) = androst-5-
CC ene-diol 3-sulfate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71099,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:190287;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Na(+)(out) + pregnenolone sulfate(out) = 2 Na(+)(in) +
CC pregnenolone sulfate(in); Xref=Rhea:RHEA:71095, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:133000; Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Na(+)(out) + taurolithocholate 3-sulfate(out) = 2 Na(+)(in)
CC + taurolithocholate 3-sulfate(in); Xref=Rhea:RHEA:71275,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:58301;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=androsterone 3alpha-sulfate(out) + 2 Na(+)(out) = androsterone
CC 3alpha-sulfate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71351,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:133003;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-dihydrotestosterone sulfate(out) + 2 Na(+)(out) =
CC 5alpha-dihydrotestosterone sulfate(in) + 2 Na(+)(in);
CC Xref=Rhea:RHEA:71355, ChEBI:CHEBI:29101, ChEBI:CHEBI:136982;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-estradiol 17-sulfate(out) + 2 Na(+)(out) = 17beta-
CC estradiol 17-sulfate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71359,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:190469;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17alpha-hydroxypregnenolone 3-sulfate(out) + 2 Na(+)(out) =
CC 17alpha-hydroxypregnenolone 3-sulfate(in) + 2 Na(+)(in);
CC Xref=Rhea:RHEA:71363, ChEBI:CHEBI:29101, ChEBI:CHEBI:133742;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=epiandrosterone 3-sulfate(out) + 2 Na(+)(out) =
CC epiandrosterone 3-sulfate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71367,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:133729;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=epitestosterone 17-sulfate(out) + 2 Na(+)(out) =
CC epitestosterone 17-sulfate(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71371,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:190485;
CC Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Na(+)(out) + testosterone 17-sulfate(out) = 2 Na(+)(in) +
CC testosterone 17-sulfate(in); Xref=Rhea:RHEA:71375, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:190489; Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=16alpha-hydroxydehydroepiandrosterone 3-sulfate(out) + 2
CC Na(+)(out) = 16alpha-hydroxydehydroepiandrosterone 3-sulfate(in) + 2
CC Na(+)(in); Xref=Rhea:RHEA:71391, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:87538; Evidence={ECO:0000250|UniProtKB:Q3KNW5};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=A6QP84-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A6QP84-2; Sequence=VSP_029095, VSP_029096;
CC Name=3;
CC IsoId=A6QP84-3; Sequence=VSP_029092;
CC Name=4;
CC IsoId=A6QP84-4; Sequence=VSP_029093, VSP_029094;
CC -!- PTM: Glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; EF186076; ABM68134.1; -; mRNA.
DR EMBL; EF495204; ABP49613.1; -; mRNA.
DR EMBL; EF495205; ABP49614.1; -; mRNA.
DR EMBL; EF495206; ABP49615.1; -; mRNA.
DR EMBL; BC149199; AAI49200.1; -; mRNA.
DR EMBL; DQ409211; ABD66746.1; -; mRNA.
DR RefSeq; NP_001075207.1; NM_001081738.1.
DR AlphaFoldDB; A6QP84; -.
DR SMR; A6QP84; -.
DR STRING; 9913.ENSBTAP00000022342; -.
DR PaxDb; A6QP84; -.
DR GeneID; 540841; -.
DR KEGG; bta:540841; -.
DR CTD; 345274; -.
DR eggNOG; KOG2718; Eukaryota.
DR HOGENOM; CLU_130202_0_0_1; -.
DR InParanoid; A6QP84; -.
DR OrthoDB; 1148347at2759; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR GO; GO:0008508; F:bile acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0043250; F:sodium-dependent organic anion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015721; P:bile acid and bile salt transport; IBA:GO_Central.
DR GO; GO:0043251; P:sodium-dependent organic anion transport; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR030203; SLC10A6.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR PANTHER; PTHR10361:SF55; PTHR10361:SF55; 1.
DR Pfam; PF01758; SBF; 1.
DR TIGRFAMs; TIGR00841; bass; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion transport; Lipid transport;
KW Membrane; Reference proteome; Sodium; Sodium transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..377
FT /note="Sodium-dependent organic anion transporter"
FT /id="PRO_0000309214"
FT TOPO_DOM 1..29
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..97
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..159
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..195
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..223
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..285
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..377
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 319..377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..339
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 126..253
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029092"
FT VAR_SEQ 127..138
FT /note="ISMTTCSTMAAL -> KERILGTHGGRA (in isoform 4)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029093"
FT VAR_SEQ 139..377
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029094"
FT VAR_SEQ 196..249
FT /note="IGAIAGGLLFLVVTGAGMVLMKEFWSSDIILLMISFIFPLIGHATGFLLALL
FT TH -> VQDNFLRNWNSECSDVLHHAAVILHCRATGPDIWLRIGLWTFPDAEWVFHGCC
FT I (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029095"
FT VAR_SEQ 250..377
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_029096"
FT CONFLICT 8
FT /note="G -> S (in Ref. 2; AAI49200)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="S -> N (in Ref. 1; ABM68134)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 41438 MW; 0257DAC7677F919C CRC64;
MRANCSSGLA CPANSSEEEL PEGLKAFGNL DLVFTVVSAL MIGLLMFSLG CSVEVQKLWG
HIRRPWGIAV GMLCQFGLMP LIAYLLIISF SLKPLQAIAV LIMGCCPGGT VSNIFTFWVD
GDMDLSISMT TCSTMAALGM MPLCLYLYTL SWNLEQNLTI PYQNIGITLV CLIIPVAFGI
YVNYRWPKQS KIILKIGAIA GGLLFLVVTG AGMVLMKEFW SSDIILLMIS FIFPLIGHAT
GFLLALLTHQ SWQRCRTISL ETGTQNVQMC FTMLQLSFTA EQLVQIFGFV LAYGLFQMLN
GFFMVAAYKM YKRRLKNKHG NEKPSCQEAR HRKKSTSPKE TTAFLEVNEE ATLSPGPSGP
VDPHGAPTPT GDIARAK
