SOB5_ARATH
ID SOB5_ARATH Reviewed; 144 AA.
AC Q9LEZ1; Q682K9;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein SUPPRESSOR OF PHYTOCHROME B 5 {ECO:0000303|PubMed:16709198};
DE Short=AtSOB5 {ECO:0000303|PubMed:16709198};
GN Name=SOB5 {ECO:0000303|PubMed:16709198};
GN OrderedLocusNames=At5g08150 {ECO:0000312|Araport:AT5G08150};
GN ORFNames=T22D6.90 {ECO:0000312|EMBL:CAB93716.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16709198; DOI=10.1111/j.1365-313x.2006.02745.x;
RA Zhang J., Wrage E.L., Vankova R., Malbeck J., Neff M.M.;
RT "Over-expression of SOB5 suggests the involvement of a novel plant protein
RT in cytokinin-mediated development.";
RL Plant J. 46:834-848(2006).
RN [6]
RP FUNCTION, MUTAGENESIS OF THR-21 AND PRO-61, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=29467189; DOI=10.1534/g3.118.200040;
RA Tayengwa R., Zhao J., Pierce C.F., Werner B.E., Neff M.M.;
RT "Synopsis of the SOFL plant-specific gene family.";
RL G3 (Bethesda) 8:1281-1290(2018).
CC -!- FUNCTION: Involved in cytokinin-mediated development (PubMed:16709198,
CC PubMed:29467189). Promotes the expression of cytokinin synthases (e.g.
CC IPT3 and IPT7), thus triggering the accumulation of trans-zeatin
CC riboside, trans-zeatin riboside monophosphate and isopentenyladenine 9-
CC glucoside (PubMed:16709198). {ECO:0000269|PubMed:16709198,
CC ECO:0000269|PubMed:29467189}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16709198,
CC ECO:0000269|PubMed:29467189}. Cell membrane
CC {ECO:0000269|PubMed:16709198}; Peripheral membrane protein
CC {ECO:0000269|PubMed:16709198}; Cytoplasmic side
CC {ECO:0000269|PubMed:16709198}. Nucleus {ECO:0000269|PubMed:29467189}.
CC -!- TISSUE SPECIFICITY: Expressed in vegetative tissues (hydathodes and
CC trichomes of leaves, shoot meristems and roots) as well as in floral
CC tissues (pistil tips, developing anthers and sepal vasculature) with
CC higher levels in young and developing tissues than in mature ones
CC (PubMed:16709198). Expressed in seedlings, leaves, flowers and
CC siliques, but not in roots (PubMed:29467189).
CC {ECO:0000269|PubMed:16709198, ECO:0000269|PubMed:29467189}.
CC -!- DOMAIN: Domains SOFL-A and SOFL-B are required for function in
CC cytokinin-mediated development. {ECO:0000250|UniProtKB:Q9CA45}.
CC -!- DISRUPTION PHENOTYPE: No obvious phenotypes.
CC {ECO:0000269|PubMed:16709198}.
CC -!- SIMILARITY: Belongs to the SOFL plant protein family. {ECO:0000305}.
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DR EMBL; AL357612; CAB93716.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91258.1; -; Genomic_DNA.
DR EMBL; BT011730; AAS49093.1; -; mRNA.
DR EMBL; AK175358; BAD43121.1; -; mRNA.
DR PIR; T50500; T50500.
DR RefSeq; NP_196432.1; NM_120898.3.
DR AlphaFoldDB; Q9LEZ1; -.
DR STRING; 3702.AT5G08150.1; -.
DR PaxDb; Q9LEZ1; -.
DR PRIDE; Q9LEZ1; -.
DR EnsemblPlants; AT5G08150.1; AT5G08150.1; AT5G08150.
DR GeneID; 830711; -.
DR Gramene; AT5G08150.1; AT5G08150.1; AT5G08150.
DR KEGG; ath:AT5G08150; -.
DR Araport; AT5G08150; -.
DR TAIR; locus:2181564; AT5G08150.
DR eggNOG; ENOG502R1MN; Eukaryota.
DR HOGENOM; CLU_1951819_0_0_1; -.
DR InParanoid; Q9LEZ1; -.
DR OMA; KANEDMV; -.
DR PRO; PR:Q9LEZ1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LEZ1; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009690; P:cytokinin metabolic process; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:UniProtKB.
DR InterPro; IPR044670; SOFL.
DR PANTHER; PTHR33347; PTHR33347; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytokinin biosynthesis; Cytokinin signaling pathway;
KW Cytoplasm; Membrane; Nucleus; Reference proteome.
FT CHAIN 1..144
FT /note="Protein SUPPRESSOR OF PHYTOCHROME B 5"
FT /id="PRO_0000450248"
FT REGION 27..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 18..23
FT /note="SOFL-A"
FT /evidence="ECO:0000305|PubMed:29467189"
FT MOTIF 52..61
FT /note="SOFL-B"
FT /evidence="ECO:0000305|PubMed:29467189"
FT COMPBIAS 35..52
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..121
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 21
FT /note="T->I: Impaired function in cytokinin-mediated
FT development; when associated with ARG-61."
FT /evidence="ECO:0000269|PubMed:29467189"
FT MUTAGEN 61
FT /note="P->R: Impaired function in cytokinin-mediated
FT development; when associated with I-21."
FT /evidence="ECO:0000269|PubMed:29467189"
FT CONFLICT 48
FT /note="D -> G (in Ref. 4; BAD43121)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 144 AA; 16200 MW; 2035B7508F476056 CRC64;
MESANIFGSD EDCRSCESGW TMYLASQSHD RDDDCYYGDD DEEEEDSDGG DSMDSDASSG
PMEATSYLKL AQEIEEQNSI KKKKKKTNEE MVLVETTRVH NNINHDDDDD DDEDDGDNHD
YDDGNDSYSV VHSYVGSVRQ KGLV
