SOFL1_ARATH
ID SOFL1_ARATH Reviewed; 148 AA.
AC Q67YG7; Q681A7; Q8LAZ9; Q9C665;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein SOB FIVE-LIKE 1 {ECO:0000303|PubMed:16709198};
DE Short=AtSOFL1 {ECO:0000303|PubMed:16709198};
GN Name=SOFL1 {ECO:0000303|PubMed:16709198};
GN OrderedLocusNames=At1g26210 {ECO:0000312|Araport:AT1G26210};
GN ORFNames=F28B23.12 {ECO:0000312|EMBL:AAG50669.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16709198; DOI=10.1111/j.1365-313x.2006.02745.x;
RA Zhang J., Wrage E.L., Vankova R., Malbeck J., Neff M.M.;
RT "Over-expression of SOB5 suggests the involvement of a novel plant protein
RT in cytokinin-mediated development.";
RL Plant J. 46:834-848(2006).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=20011053; DOI=10.1371/journal.pone.0008236;
RA Zhang J., Vankova R., Malbeck J., Dobrev P.I., Xu Y., Chong K., Neff M.M.;
RT "AtSOFL1 and AtSOFL2 act redundantly as positive modulators of the
RT endogenous content of specific cytokinins in Arabidopsis.";
RL PLoS ONE 4:e8236-e8236(2009).
RN [8]
RP FUNCTION, MUTAGENESIS OF THR-23 AND PRO-84, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=29467189; DOI=10.1534/g3.118.200040;
RA Tayengwa R., Zhao J., Pierce C.F., Werner B.E., Neff M.M.;
RT "Synopsis of the SOFL plant-specific gene family.";
RL G3 (Bethesda) 8:1281-1290(2018).
CC -!- FUNCTION: Involved in cytokinin-mediated development (PubMed:20011053,
CC PubMed:29467189). Together with SOFL2, triggers the endogenous content
CC of specific bioactive cytokinins derived from the biosynthetic
CC intermediates trans-zeatin riboside monophosphate (tZRMP) and N(6)-
CC (Delta(2)-isopentenyl)adenosine monophosphate (iPRMP) such as N-
CC glucosides trans-zeatin 7-glucoside (tZ7G), cis-zeatin 7-glucoside
CC (cZ7G) and N(6)-(Delta(2)-isopentenyl)adenine 7-glucoside (iP7G)
CC (PubMed:20011053). {ECO:0000269|PubMed:20011053,
CC ECO:0000269|PubMed:29467189}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29467189}. Nucleus
CC {ECO:0000269|PubMed:29467189}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q67YG7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q67YG7-2; Sequence=Not described;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the vascular tissues of
CC seedlings, developing leaves, flowers and siliques, but barely
CC detectable in roots and stems. {ECO:0000269|PubMed:20011053,
CC ECO:0000269|PubMed:29467189}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, strong levels in the hydathode
CC region of cotyledons as well as in the upper part of hypocotyls and
CC weak content in the vascular tissue of cotyledons (PubMed:20011053). In
CC developing leaves, strongly expressed in the midrib of leaf veins, but
CC weak levels in the hydathode regions (PubMed:20011053). In developing
CC flower buds, accumulates in pistil tips and the vascular tissue of
CC stamens and sepals (PubMed:20011053). In mature flowers, detected in
CC the vascular bundles between the two anther locules, the central
CC vascular cylinders of the filaments, vascular tissues of tips and bases
CC of the pistils, and sepal vascular tissue (PubMed:20011053). Also
CC present in tips and bases of developing siliques, and progressively
CC restricted to the vascular tissues at the silique tips
CC (PubMed:20011053). {ECO:0000269|PubMed:20011053}.
CC -!- DOMAIN: Domains SOFL-A and SOFL-B are required for function in
CC cytokinin-mediated development. {ECO:0000250|UniProtKB:Q9CA45}.
CC -!- DISRUPTION PHENOTYPE: Plants missing both SOLF1 and SOLF2 have reduced
CC endogenous cytokinin levels and accumulate lower levels of trans-zeatin
CC riboside monophosphate (tZRMP) and N(6)-(Delta(2)-isopentenyl)adenosine
CC monophosphate (iPRMP), biosynthetic intermediates of bioactive
CC cytokinins as well as decreased response to exogenous cytokinin in both
CC callus-formation and inhibition-of-hypocotyl-elongation assays.
CC {ECO:0000269|PubMed:20011053}.
CC -!- SIMILARITY: Belongs to the SOFL plant protein family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50669.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC079829; AAG50669.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30661.1; -; Genomic_DNA.
DR EMBL; AK175479; BAD43242.1; -; mRNA.
DR EMBL; AK175710; BAD43473.1; -; mRNA.
DR EMBL; AK176501; BAD44264.1; -; mRNA.
DR EMBL; BT024679; ABD57504.1; -; mRNA.
DR EMBL; AY087508; AAM65051.1; -; mRNA.
DR PIR; C86388; C86388.
DR RefSeq; NP_564241.1; NM_102385.2. [Q67YG7-1]
DR AlphaFoldDB; Q67YG7; -.
DR STRING; 3702.AT1G26210.1; -.
DR PaxDb; Q67YG7; -.
DR PRIDE; Q67YG7; -.
DR EnsemblPlants; AT1G26210.1; AT1G26210.1; AT1G26210. [Q67YG7-1]
DR GeneID; 839162; -.
DR Gramene; AT1G26210.1; AT1G26210.1; AT1G26210. [Q67YG7-1]
DR KEGG; ath:AT1G26210; -.
DR Araport; AT1G26210; -.
DR TAIR; locus:2028792; AT1G26210.
DR HOGENOM; CLU_1858027_0_0_1; -.
DR InParanoid; Q67YG7; -.
DR OMA; IEDAFHG; -.
DR OrthoDB; 1592862at2759; -.
DR PhylomeDB; Q67YG7; -.
DR PRO; PR:Q67YG7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q67YG7; differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009690; P:cytokinin metabolic process; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:UniProtKB.
DR InterPro; IPR044670; SOFL.
DR PANTHER; PTHR33347; PTHR33347; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytokinin biosynthesis; Cytokinin signaling pathway;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..148
FT /note="Protein SOB FIVE-LIKE 1"
FT /id="PRO_0000450249"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 20..25
FT /note="SOFL-A"
FT /evidence="ECO:0000305|PubMed:29467189"
FT MOTIF 75..84
FT /note="SOFL-B"
FT /evidence="ECO:0000305|PubMed:29467189"
FT COMPBIAS 39..76
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..130
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 23
FT /note="T->I: Impaired function in cytokinin-mediated
FT development; when associated with R-84."
FT /evidence="ECO:0000269|PubMed:29467189"
FT MUTAGEN 84
FT /note="P->R: Impaired function in cytokinin-mediated
FT development; when associated with I-23."
FT /evidence="ECO:0000269|PubMed:29467189"
FT CONFLICT 35
FT /note="Q -> L (in Ref. 3; BAD43473)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="D -> N (in Ref. 5; AAM65051)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 148 AA; 16249 MW; C0DD7F92C3A745E0 CRC64;
MESPRNHGGS EEEEYSSCES GWTMYIEDAF HGNDQSSVVV DDDDDDTQVK EADDGYENDD
GDTSDDGGDE ESDDSMASDA SSGPSNQLPK HINKHAARKN GSKQVYLQKR QHTEKTISNE
GEKSDLKART RTSAASRVQS RGKVSKTK
