SOFL2_ARATH
ID SOFL2_ARATH Reviewed; 147 AA.
AC Q9CA45;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Protein SOB FIVE-LIKE 2 {ECO:0000303|PubMed:16709198};
DE Short=AtSOFL2 {ECO:0000303|PubMed:16709198};
GN Name=SOFL2 {ECO:0000303|PubMed:16709198};
GN OrderedLocusNames=At1g68870 {ECO:0000312|Araport:AT1G68870};
GN ORFNames=F14K14.2 {ECO:0000312|EMBL:AAG52031.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16709198; DOI=10.1111/j.1365-313x.2006.02745.x;
RA Zhang J., Wrage E.L., Vankova R., Malbeck J., Neff M.M.;
RT "Over-expression of SOB5 suggests the involvement of a novel plant protein
RT in cytokinin-mediated development.";
RL Plant J. 46:834-848(2006).
RN [5]
RP FUNCTION, MUTAGENESIS OF THR-21 AND ASP-80, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=20011053; DOI=10.1371/journal.pone.0008236;
RA Zhang J., Vankova R., Malbeck J., Dobrev P.I., Xu Y., Chong K., Neff M.M.;
RT "AtSOFL1 and AtSOFL2 act redundantly as positive modulators of the
RT endogenous content of specific cytokinins in Arabidopsis.";
RL PLoS ONE 4:e8236-e8236(2009).
RN [6]
RP FUNCTION, MUTAGENESIS OF THR-21 AND ASP-80, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=29467189; DOI=10.1534/g3.118.200040;
RA Tayengwa R., Zhao J., Pierce C.F., Werner B.E., Neff M.M.;
RT "Synopsis of the SOFL plant-specific gene family.";
RL G3 (Bethesda) 8:1281-1290(2018).
CC -!- FUNCTION: Involved in cytokinin-mediated development (PubMed:20011053,
CC PubMed:29467189). Together with SOFL2, triggers the endogenous content
CC of specific bioactive cytokinins derived from the biosynthetic
CC intermediates trans-zeatin riboside monophosphate (tZRMP) and N(6)-
CC (Delta(2)-isopentenyl)adenosine monophosphate (iPRMP) such as N-
CC glucosides trans-zeatin 7-glucoside (tZ7G), cis-zeatin 7-glucoside
CC (cZ7G) and N(6)-(Delta(2)-isopentenyl)adenine 7-glucoside (iP7G)
CC (PubMed:20011053). {ECO:0000269|PubMed:20011053,
CC ECO:0000269|PubMed:29467189}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29467189}. Nucleus
CC {ECO:0000269|PubMed:29467189}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the vascular tissues of
CC seedlings, developing leaves, flowers and siliques, but barely
CC detectable in roots and stems. {ECO:0000269|PubMed:20011053,
CC ECO:0000269|PubMed:29467189}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, strong levels in the hydathode
CC region of cotyledons as well as in the upper part of hypocotyls and
CC weak content in the vascular tissue of cotyledons (PubMed:20011053). In
CC developing leaves, strongly expressed in the midrib of leaf veins, but
CC weak levels in the hydathode regions (PubMed:20011053). In developing
CC flower buds, accumulates in pistil tips and the vascular tissue of
CC stamens and sepals (PubMed:20011053). In mature flowers, detected in
CC the vascular bundles between the two anther locules, the central
CC vascular cylinders of the filaments, vascular tissues of tips of the
CC pistils, and sepal vascular tissue (PubMed:20011053). Also present in
CC tips and bases of developing siliques, and progressively restricted to
CC the vascular tissues at the silique tips (PubMed:20011053).
CC {ECO:0000269|PubMed:20011053}.
CC -!- DOMAIN: Domains SOFL-A and SOFL-B are required for function in
CC cytokinin-mediated development. {ECO:0000269|PubMed:20011053}.
CC -!- DISRUPTION PHENOTYPE: No obvious developmental defects
CC (PubMed:20011053). Plants missing both SOLF1 and SOLF2 have reduced
CC endogenous cytokinin levels and accumulate lower levels of trans-zeatin
CC riboside monophosphate (tZRMP) and N(6)-(Delta(2)-isopentenyl)adenosine
CC monophosphate (iPRMP), biosynthetic intermediates of bioactive
CC cytokinins as well as decreased response to exogenous cytokinin in both
CC callus-formation and inhibition-of-hypocotyl-elongation assays
CC (PubMed:20011053). {ECO:0000269|PubMed:20011053}.
CC -!- SIMILARITY: Belongs to the SOFL plant protein family. {ECO:0000305}.
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DR EMBL; AC011914; AAG52031.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34852.1; -; Genomic_DNA.
DR EMBL; BT014768; AAT41751.1; -; mRNA.
DR EMBL; BT015000; AAT70451.1; -; mRNA.
DR RefSeq; NP_177053.1; NM_105560.3.
DR AlphaFoldDB; Q9CA45; -.
DR STRING; 3702.AT1G68870.1; -.
DR PaxDb; Q9CA45; -.
DR PRIDE; Q9CA45; -.
DR ProteomicsDB; 183353; -.
DR EnsemblPlants; AT1G68870.1; AT1G68870.1; AT1G68870.
DR GeneID; 843219; -.
DR Gramene; AT1G68870.1; AT1G68870.1; AT1G68870.
DR KEGG; ath:AT1G68870; -.
DR Araport; AT1G68870; -.
DR TAIR; locus:2012418; AT1G68870.
DR HOGENOM; CLU_1858027_0_0_1; -.
DR InParanoid; Q9CA45; -.
DR OMA; NAKQVNH; -.
DR OrthoDB; 1578912at2759; -.
DR PhylomeDB; Q9CA45; -.
DR PRO; PR:Q9CA45; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CA45; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009690; P:cytokinin metabolic process; IMP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:UniProtKB.
DR InterPro; IPR044670; SOFL.
DR PANTHER; PTHR33347; PTHR33347; 1.
PE 1: Evidence at protein level;
KW Cytokinin biosynthesis; Cytokinin signaling pathway; Cytoplasm; Nucleus;
KW Reference proteome.
FT CHAIN 1..147
FT /note="Protein SOB FIVE-LIKE 2"
FT /id="PRO_0000450250"
FT REGION 32..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 18..23
FT /note="SOFL-A"
FT /evidence="ECO:0000305|PubMed:29467189"
FT MOTIF 76..85
FT /note="SOFL-B"
FT /evidence="ECO:0000305|PubMed:29467189"
FT COMPBIAS 44..73
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..135
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 21
FT /note="T->I: Impaired function in cytokinin-mediated
FT development; when associated with N-80."
FT /evidence="ECO:0000269|PubMed:20011053,
FT ECO:0000269|PubMed:29467189"
FT MUTAGEN 80
FT /note="D->N: Impaired function in cytokinin-mediated
FT development; when associated with I-21."
FT /evidence="ECO:0000269|PubMed:20011053,
FT ECO:0000269|PubMed:29467189"
SQ SEQUENCE 147 AA; 16389 MW; BF35DE68AE525213 CRC64;
MESPRIHGGA EEKSSCESGW TMYIEDTFHG NHHSEVVYEE EDDGFSVKEV DDDGDGDEDD
DDDDDDDSSN NESDDSMTSD ASSWPSTHQP PRSTKNHAAA KNSNAKQVNN QTENRVRDRF
SDEGEESELK ARTRTTAASR VKVSKTK
