ID   SOHB_BUCAI              Reviewed;         336 AA.
AC   P57370;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Probable protease SohB;
DE            EC=3.4.21.-;
GN   Name=sohB; OrderedLocusNames=BU283;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Possible protease. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB12993.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000003; BAB12993.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_240107.2; NC_002528.1.
DR   RefSeq; WP_010896045.1; NC_002528.1.
DR   AlphaFoldDB; P57370; -.
DR   SMR; P57370; -.
DR   STRING; 107806.10038958; -.
DR   EnsemblBacteria; BAB12993; BAB12993; BAB12993.
DR   KEGG; buc:BU283; -.
DR   PATRIC; fig|107806.10.peg.293; -.
DR   eggNOG; COG0616; Bacteria.
DR   HOGENOM; CLU_070316_0_0_6; -.
DR   OMA; FLYEYGL; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07023; S49_Sppa_N_C; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR002142; Peptidase_S49.
DR   InterPro; IPR013703; Peptidase_S49_N_proteobac.
DR   Pfam; PF01343; Peptidase_S49; 1.
DR   Pfam; PF08496; Peptidase_S49_N; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Protease; Reference proteome;
KW   Serine protease; Transmembrane; Transmembrane helix.
FT   CHAIN           1..336
FT                   /note="Probable protease SohB"
FT                   /id="PRO_0000171444"
FT   TRANSMEM        10..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        167
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        219
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   336 AA;  38676 MW;  A57C8219479B2F1F CRC64;
     MNLLLNYELF LAKIITFIII SISILILFYT IIKRKKNIQS KIKITLLQDN YKNVKNKILL
     STMKNVEKKI WFKKQKEKNK KELLLKNNKK KLFVLDFKGD VYANEVVGLR EEISAILLVA
     NKHDEVLLRL ESSGGVIHGY GLAASQLNRL RQKGIRLIVS VDKIAASGGY MMACVADYIV
     SAPFAIIGSI GVVGQIPNFN KLLKKCNIDF ELHTAGDYKR TLTMFGNNTE STRKKFCDEL
     NTTHKLFKSF IKEMRPSLDI EDVSNGEHWF GTIALEKKLV DQIGTSDDIL ISKMEEYTLL
     RIQYIYRKKI LERFTASVTH NLSETLLKIF FYKNYL