ID   SOHB_BUCAP              Reviewed;         342 AA.
AC   Q8K9P8;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Probable protease SohB;
DE            EC=3.4.21.-;
GN   Name=sohB; OrderedLocusNames=BUsg_272;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Possible protease. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family. {ECO:0000305}.
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DR   EMBL; AE013218; AAM67830.1; -; Genomic_DNA.
DR   RefSeq; WP_011053797.1; NC_004061.1.
DR   AlphaFoldDB; Q8K9P8; -.
DR   SMR; Q8K9P8; -.
DR   STRING; 198804.BUsg_272; -.
DR   EnsemblBacteria; AAM67830; AAM67830; BUsg_272.
DR   KEGG; bas:BUsg_272; -.
DR   eggNOG; COG0616; Bacteria.
DR   HOGENOM; CLU_070316_0_0_6; -.
DR   OMA; FLYEYGL; -.
DR   OrthoDB; 1318587at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07023; S49_Sppa_N_C; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR002142; Peptidase_S49.
DR   InterPro; IPR013703; Peptidase_S49_N_proteobac.
DR   Pfam; PF01343; Peptidase_S49; 1.
DR   Pfam; PF08496; Peptidase_S49_N; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Hydrolase; Membrane; Protease; Serine protease;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..342
FT                   /note="Probable protease SohB"
FT                   /id="PRO_0000171445"
FT   TRANSMEM        10..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        172
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        224
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   342 AA;  39736 MW;  9D2696175AE0D635 CRC64;
     MNLLLNYELF LAKAITFLFI IFITPFIFNI IKRKRTDQKK FKIILLEEKY KNIKKDILLS
     KMNKLEKKKW IKEEKKKDKE FEKKNKNNIV TLKKKTLFVL DFKGGIHAHE VIGLREEISA
     ILLAANKDDE VLLRLESSGG VIHGYGLAAA QLERLRQNKI RLIISIDKIA ASGGYMMACV
     ADYIISAPFA IIGSIGVVGQ LPNFNKLLKK CNIDVELHTA GDYKRTLTMF GQNTELTRKK
     FCQELNLTHE IFKKFIKKMR PCLDIENISN GEHWFGTIAF KKNLVDEINT SDNILMSKMK
     EKYTLLNIQY IYKNKKLENF TSFIIENIKI IIIKIFSYKK IL