SOHB_ECOLI
ID SOHB_ECOLI Reviewed; 349 AA.
AC P0AG14; P24213; P77676;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Probable protease SohB;
DE EC=3.4.21.-;
GN Name=sohB; OrderedLocusNames=b1272, JW1264;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1885549; DOI=10.1128/jb.173.18.5763-5770.1991;
RA Baird L., Lipinska B., Raina S., Georgopoulos C.;
RT "Identification of the Escherichia coli sohB gene, a multicopy suppressor
RT of the HtrA (DegP) null phenotype.";
RL J. Bacteriol. 173:5763-5770(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 133-258.
RX PubMed=3029379; DOI=10.1016/0022-2836(86)90129-4;
RA Tse-Dinh Y.-C., Wang J.C.;
RT "Complete nucleotide sequence of the topA gene encoding Escherichia coli
RT DNA topoisomerase I.";
RL J. Mol. Biol. 191:321-331(1986).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Multicopy suppressor of the HtrA (DegP) null phenotype. It is
CC possibly a protease, not essential for bacterial viability.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Single-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the peptidase S49 family. {ECO:0000305}.
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DR EMBL; M73320; AAA24639.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74354.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA14809.1; -; Genomic_DNA.
DR PIR; C64875; C64875.
DR RefSeq; NP_415788.1; NC_000913.3.
DR RefSeq; WP_000422045.1; NZ_STEB01000005.1.
DR AlphaFoldDB; P0AG14; -.
DR SMR; P0AG14; -.
DR BioGRID; 4259577; 67.
DR DIP; DIP-35930N; -.
DR IntAct; P0AG14; 3.
DR STRING; 511145.b1272; -.
DR MEROPS; S49.002; -.
DR jPOST; P0AG14; -.
DR PaxDb; P0AG14; -.
DR PRIDE; P0AG14; -.
DR EnsemblBacteria; AAC74354; AAC74354; b1272.
DR EnsemblBacteria; BAA14809; BAA14809; BAA14809.
DR GeneID; 66674906; -.
DR GeneID; 945858; -.
DR KEGG; ecj:JW1264; -.
DR KEGG; eco:b1272; -.
DR PATRIC; fig|1411691.4.peg.1012; -.
DR EchoBASE; EB0949; -.
DR eggNOG; COG0616; Bacteria.
DR HOGENOM; CLU_070316_0_0_6; -.
DR InParanoid; P0AG14; -.
DR OMA; FLYEYGL; -.
DR PhylomeDB; P0AG14; -.
DR BioCyc; EcoCyc:EG10956-MON; -.
DR PRO; PR:P0AG14; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07023; S49_Sppa_N_C; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR002142; Peptidase_S49.
DR InterPro; IPR013703; Peptidase_S49_N_proteobac.
DR Pfam; PF01343; Peptidase_S49; 1.
DR Pfam; PF08496; Peptidase_S49_N; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Hydrolase; Membrane; Protease;
KW Reference proteome; Serine protease; Transmembrane; Transmembrane helix.
FT CHAIN 1..349
FT /note="Probable protease SohB"
FT /id="PRO_0000171448"
FT TOPO_DOM 1..8
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..349
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT ACT_SITE 178
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 230
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT CONFLICT 85
FT /note="A -> R (in Ref. 1; AAA24639)"
FT /evidence="ECO:0000305"
FT CONFLICT 90
FT /note="A -> R (in Ref. 1; AAA24639)"
FT /evidence="ECO:0000305"
FT CONFLICT 133..138
FT /note="PQDQVV -> HQPQVA (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 143..154
FT /note="SPGGMVHGYGLA -> ALVAWCMLRAG (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="K -> R (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 245..258
FT /note="EKFREELNETHQLF -> GKIPRRVERNASVV (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 296
FT /note="T -> A (in Ref. 1; AAA24639)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 349 AA; 39366 MW; 3362AE74C33C5FFC CRC64;
MELLSEYGLF LAKIVTVVLA IAAIAAIIVN VAQRNKRQRG ELRVNNLSEQ YKEMKEELAA
ALMDSHQQKQ WHKAQKKKHK QEAKAAKAKA KLGEVATDSK PRVWVLDFKG SMDAHEVNSL
REEITAVLAA FKPQDQVVLR LESPGGMVHG YGLAASQLQR LRDKNIPLTV TVDKVAASGG
YMMACVADKI VSAPFAIVGS IGVVAQMPNF NRFLKSKDID IELHTAGQYK RTLTLLGENT
EEGREKFREE LNETHQLFKD FVKRMRPSLD IEQVATGEHW YGQQAVEKGL VDEINTSDEV
ILSLMEGREV VNVRYMQRKR LIDRFTGSAA ESADRLLLRW WQRGQKPLM
