ID   SOHB_SHIFL              Reviewed;         349 AA.
AC   P0AG15; P24213; P77676;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Probable protease SohB;
DE            EC=3.4.21.-;
GN   Name=sohB; OrderedLocusNames=SF1274, S1359;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Multicopy suppressor of the HtrA (DegP) null phenotype. It is
CC       possibly a protease, not essential for bacterial viability (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family. {ECO:0000305}.
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DR   EMBL; AE005674; AAN42886.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP16772.1; -; Genomic_DNA.
DR   RefSeq; NP_707179.1; NC_004337.2.
DR   RefSeq; WP_000422045.1; NZ_UIQL01000016.1.
DR   AlphaFoldDB; P0AG15; -.
DR   SMR; P0AG15; -.
DR   STRING; 198214.SF1274; -.
DR   PRIDE; P0AG15; -.
DR   EnsemblBacteria; AAN42886; AAN42886; SF1274.
DR   EnsemblBacteria; AAP16772; AAP16772; S1359.
DR   GeneID; 1024236; -.
DR   GeneID; 66674906; -.
DR   KEGG; sfl:SF1274; -.
DR   KEGG; sfx:S1359; -.
DR   PATRIC; fig|198214.7.peg.1495; -.
DR   HOGENOM; CLU_070316_0_0_6; -.
DR   OMA; FLYEYGL; -.
DR   OrthoDB; 1318587at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07023; S49_Sppa_N_C; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR002142; Peptidase_S49.
DR   InterPro; IPR013703; Peptidase_S49_N_proteobac.
DR   Pfam; PF01343; Peptidase_S49; 1.
DR   Pfam; PF08496; Peptidase_S49_N; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Protease;
KW   Reference proteome; Serine protease; Transmembrane; Transmembrane helix.
FT   CHAIN           1..349
FT                   /note="Probable protease SohB"
FT                   /id="PRO_0000171450"
FT   TOPO_DOM        1..8
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..349
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        178
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        230
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   349 AA;  39366 MW;  3362AE74C33C5FFC CRC64;
     MELLSEYGLF LAKIVTVVLA IAAIAAIIVN VAQRNKRQRG ELRVNNLSEQ YKEMKEELAA
     ALMDSHQQKQ WHKAQKKKHK QEAKAAKAKA KLGEVATDSK PRVWVLDFKG SMDAHEVNSL
     REEITAVLAA FKPQDQVVLR LESPGGMVHG YGLAASQLQR LRDKNIPLTV TVDKVAASGG
     YMMACVADKI VSAPFAIVGS IGVVAQMPNF NRFLKSKDID IELHTAGQYK RTLTLLGENT
     EEGREKFREE LNETHQLFKD FVKRMRPSLD IEQVATGEHW YGQQAVEKGL VDEINTSDEV
     ILSLMEGREV VNVRYMQRKR LIDRFTGSAA ESADRLLLRW WQRGQKPLM