ID   SOMA_BUBBU              Reviewed;         217 AA.
AC   O18938;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 3.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Somatotropin;
DE   AltName: Full=Growth hormone;
DE   Flags: Precursor;
GN   Name=GH1; Synonyms=GH;
OS   Bubalus bubalis (Domestic water buffalo).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bubalus.
OX   NCBI_TaxID=89462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Tiwari G., Garg L.C.;
RT   "Cloning and characterization of growth hormone encoding gene in Bubalus
RT   bubalis.";
RL   Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Blood;
RX   PubMed=10376211; DOI=10.3109/10425179909008425;
RA   Verma S., Ghorpade A., Tiwari G., Das P., Garg L.C.;
RT   "cDNA cloning and sequence analysis of bubaline growth hormone.";
RL   DNA Seq. 10:101-103(1999).
CC   -!- FUNCTION: Plays an important role in growth control. Its major role in
CC       stimulating body growth is to stimulate the liver and other tissues to
CC       secrete IGF-1. It stimulates both the differentiation and proliferation
CC       of myoblasts. It also stimulates amino acid uptake and protein
CC       synthesis in muscle and other tissues.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the somatotropin/prolactin family.
CC       {ECO:0000305}.
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DR   EMBL; AJ011533; CAA09679.1; -; Genomic_DNA.
DR   EMBL; AJ011514; CAA09668.1; -; Genomic_DNA.
DR   EMBL; AJ011513; CAA09667.1; -; Genomic_DNA.
DR   EMBL; AJ000549; CAA04181.1; -; Genomic_DNA.
DR   EMBL; X72947; CAA51450.1; -; mRNA.
DR   PIR; S32682; S32682.
DR   AlphaFoldDB; O18938; -.
DR   BMRB; O18938; -.
DR   SMR; O18938; -.
DR   GO; GO:0005615; C:extracellular space; ISS:AgBase.
DR   GO; GO:0005131; F:growth hormone receptor binding; IEA:InterPro.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030073; P:insulin secretion; ISS:AgBase.
DR   GO; GO:0009893; P:positive regulation of metabolic process; IEA:UniProt.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR034975; Somatotropin.
DR   InterPro; IPR001400; Somatotropin/Prolactin.
DR   InterPro; IPR018116; Somatotropin_CS.
DR   PANTHER; PTHR11417; PTHR11417; 1.
DR   PANTHER; PTHR11417:SF2; PTHR11417:SF2; 1.
DR   Pfam; PF00103; Hormone_1; 1.
DR   PRINTS; PR00836; SOMATOTROPIN.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00266; SOMATOTROPIN_1; 1.
DR   PROSITE; PS00338; SOMATOTROPIN_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Hormone; Metal-binding; Phosphoprotein; Secreted; Signal;
KW   Zinc.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000250"
FT   CHAIN           27..217
FT                   /note="Somatotropin"
FT                   /id="PRO_0000032975"
FT   BINDING         46
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         199
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         132
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P01241"
FT   DISULFID        79..190
FT                   /evidence="ECO:0000250"
FT   DISULFID        207..215
FT                   /evidence="ECO:0000250"
FT   CONFLICT        15
FT                   /note="A -> T (in Ref. 1; CAA51450)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        35
FT                   /note="S -> G (in Ref. 1; CAA51450)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        43
FT                   /note="W -> R (in Ref. 1; CAA51450)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        156
FT                   /note="G -> V (in Ref. 1; CAA51450)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   217 AA;  24618 MW;  453547080E9B54EB CRC64;
     MMAAGPRTSL LLAFALLCLP WTQVVGAFPA MSLSSLFANA VLWAQHLHQL AADTFKEFER
     TYIPEGQRYS IQNTQVAFCF SETIPAPTGK NEAQQKSDLE LLRISLLLIQ SWLGPLQFLS
     RVFTNSLVFG TSDRVYEKLK DLEEGILALM RELEDGTPRA GQILKQTYDK FDTNMRSDDA
     LLKNYGLLSC FRKDLHKTET YLRVMKCRRF GEASCAF