ID   SOMA_LITCT              Reviewed;         215 AA.
AC   P10813;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Somatotropin;
DE   AltName: Full=Growth hormone;
DE   Flags: Precursor;
GN   Name=GH;
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=3260110; DOI=10.1016/0167-4781(88)90017-6;
RA   Pan F.-M., Chang W.-C.;
RT   "Cloning and sequencing of bullfrog growth hormone complementary DNA.";
RL   Biochim. Biophys. Acta 950:238-242(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=1476615; DOI=10.1677/jme.0.0090283;
RA   Takahashi N., Kikuyama S., Gen K., Maruyama O., Kato Y.;
RT   "Cloning of a bullfrog growth hormone cDNA: expression of growth hormone
RT   mRNA in larval and adult bullfrog pituitaries.";
RL   J. Mol. Endocrinol. 9:283-289(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 26-215.
RC   TISSUE=Pituitary;
RX   PubMed=1859828; DOI=10.1016/0167-4838(91)90160-2;
RA   Kobayashi T., Yasuda A., Yamaguchi K., Kawauchi H., Kikuyama S.;
RT   "The complete amino acid sequence of growth hormone of the bullfrog (Rana
RT   catesbeiana).";
RL   Biochim. Biophys. Acta 1078:383-387(1991).
CC   -!- FUNCTION: Growth hormone plays an important role in growth control.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DEVELOPMENTAL STAGE: Levels increase as metamorphosis progresses, reach
CC       maxima in juveniles and decrease as adulthood approaches.
CC   -!- SIMILARITY: Belongs to the somatotropin/prolactin family.
CC       {ECO:0000305}.
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DR   EMBL; X12520; CAA31038.1; -; mRNA.
DR   EMBL; S52027; AAB24792.1; -; mRNA.
DR   PIR; I51188; I51188.
DR   PIR; JS0037; JS0037.
DR   AlphaFoldDB; P10813; -.
DR   SMR; P10813; -.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0005131; F:growth hormone receptor binding; IEA:InterPro.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR034975; Somatotropin.
DR   InterPro; IPR001400; Somatotropin/Prolactin.
DR   InterPro; IPR018116; Somatotropin_CS.
DR   PANTHER; PTHR11417; PTHR11417; 1.
DR   PANTHER; PTHR11417:SF2; PTHR11417:SF2; 1.
DR   Pfam; PF00103; Hormone_1; 1.
DR   PRINTS; PR00836; SOMATOTROPIN.
DR   SUPFAM; SSF47266; SSF47266; 1.
DR   PROSITE; PS00266; SOMATOTROPIN_1; 1.
DR   PROSITE; PS00338; SOMATOTROPIN_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hormone; Metal-binding;
KW   Secreted; Signal; Zinc.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:1859828"
FT   CHAIN           26..215
FT                   /note="Somatotropin"
FT                   /id="PRO_0000033008"
FT   BINDING         44
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         197
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   DISULFID        77..188
FT                   /evidence="ECO:0000250"
FT   DISULFID        205..213
FT                   /evidence="ECO:0000250"
FT   CONFLICT        68..73
FT                   /note="SNKHSY -> KQTLLI (in Ref. 1; CAA31038)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        98
FT                   /note="D -> E (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        105
FT                   /note="T -> L (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        112
FT                   /note="T -> N (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   215 AA;  24975 MW;  3C08D5840EFF102A CRC64;
     MASGLGSSLV LLVVICLQSP QGFNAFPQMS LSNLFTNAVI RAQHLHQMVA DTYRDYERTY
     IPEDQRFSNK HSYSVYCYSE TIPAPTDKDN THQKSDIDLL RFSLTLLQSW MTPIQIVNRV
     FGNNQVFGNI DRVYDRLRDL DEGLHILIRE LDDGNVRNYG VLTFTYDKFD VNLRSEEGRA
     KNYGLLSCFK KDMHKVETYL KVMKCRRFVE SNCTF