SON_DROME
ID SON_DROME Reviewed; 874 AA.
AC Q9VHB0; Q8IHA8; Q8MSG7;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Protein Son {ECO:0000305};
DE AltName: Full=RNA-binding protein Son {ECO:0000312|FlyBase:FBgn0037716};
GN Name=Son {ECO:0000312|FlyBase:FBgn0037716};
GN Synonyms=dsn {ECO:0000303|PubMed:29887366, ECO:0000303|PubMed:31730657,
GN ECO:0000312|FlyBase:FBgn0037716};
GN ORFNames=CG8273 {ECO:0000312|FlyBase:FBgn0037716};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM50693.1, ECO:0000312|EMBL:AAN71087.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM50693.1, ECO:0000312|EMBL:AAN71087.1};
RC TISSUE=Ovary {ECO:0000312|EMBL:AAM50693.1}, and
RC Testis {ECO:0000312|EMBL:AAN71087.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=29887366; DOI=10.1016/j.stemcr.2018.05.005;
RA Ng A.Y.E., Peralta K.R.G., Pek J.W.;
RT "Germline Stem Cell Heterogeneity Supports Homeostasis in Drosophila.";
RL Stem Cell Reports 11:13-21(2018).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=31730657; DOI=10.1371/journal.pgen.1008498;
RA Tay M.L., Pek J.W.;
RT "SON protects nascent transcripts from unproductive degradation by
RT counteracting DIP1.";
RL PLoS Genet. 15:E1008498-E1008498(2019).
CC -!- FUNCTION: RNA-binding protein that protects nascent transcripts
CC containing intronic transposable sequences, known as INE-1, from being
CC degraded by DIP1 (PubMed:31730657). Modulates DIP1 activity by
CC repressing its sumoylation levels (PubMed:31730657). This ensures that
CC intronic sequences will be degradated only after splicing
CC (PubMed:31730657). In the ovaries, regulates germline stem cells (GSCs)
CC self-renewal by repressing the expression of the GSC differentiation-
CC promoting factor Rga (PubMed:29887366). {ECO:0000269|PubMed:29887366,
CC ECO:0000269|PubMed:31730657}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31730657}.
CC Note=Localizes to DIP1-positive nuclear bodies known as satellite
CC bodies. {ECO:0000269|PubMed:31730657}.
CC -!- TISSUE SPECIFICITY: Expressed in ovarian nurse cells (at protein
CC level). {ECO:0000269|PubMed:29887366, ECO:0000269|PubMed:31730657}.
CC -!- DISRUPTION PHENOTYPE: Results in a progressing reduction in egg laying
CC accompanied by a decrease in number of germline stem cells (GSCs)
CC (PubMed:29887366). In the ovaries, results in an increase of Rga pre-
CC mRNA (PubMed:29887366). Results in a decrease in the levels and
CC stability of mRNA and INE-1-containing pre-RNA (PubMed:31730657). Might
CC reduce levels of DIP1 sumoylation (PubMed:31730657). RNAi-mediated
CC knockdown in the germline results in loss of GSCs (PubMed:29887366).
CC {ECO:0000269|PubMed:29887366, ECO:0000269|PubMed:31730657}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM50693.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF54409.1; -; Genomic_DNA.
DR EMBL; AE014297; AGB95808.1; -; Genomic_DNA.
DR EMBL; AY118833; AAM50693.1; ALT_INIT; mRNA.
DR EMBL; BT001332; AAN71087.1; -; mRNA.
DR RefSeq; NP_001262426.1; NM_001275497.1.
DR RefSeq; NP_649914.1; NM_141657.4.
DR AlphaFoldDB; Q9VHB0; -.
DR SMR; Q9VHB0; -.
DR IntAct; Q9VHB0; 6.
DR STRING; 7227.FBpp0081550; -.
DR PaxDb; Q9VHB0; -.
DR PRIDE; Q9VHB0; -.
DR ABCD; Q9VHB0; 3 sequenced antibodies.
DR EnsemblMetazoa; FBtr0082072; FBpp0081550; FBgn0037716.
DR EnsemblMetazoa; FBtr0334655; FBpp0306717; FBgn0037716.
DR GeneID; 41159; -.
DR KEGG; dme:Dmel_CG8273; -.
DR UCSC; CG8273-RA; d. melanogaster.
DR CTD; 6651; -.
DR FlyBase; FBgn0037716; Son.
DR VEuPathDB; VectorBase:FBgn0037716; -.
DR eggNOG; ENOG502QPQ7; Eukaryota.
DR GeneTree; ENSGT00730000111141; -.
DR HOGENOM; CLU_015129_0_0_1; -.
DR InParanoid; Q9VHB0; -.
DR OMA; HLPGQFT; -.
DR OrthoDB; 547669at2759; -.
DR PhylomeDB; Q9VHB0; -.
DR SignaLink; Q9VHB0; -.
DR BioGRID-ORCS; 41159; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41159; -.
DR PRO; PR:Q9VHB0; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037716; Expressed in ovary and 12 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003725; F:double-stranded RNA binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0036099; P:female germ-line stem cell population maintenance; IMP:UniProtKB.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0033234; P:negative regulation of protein sumoylation; IMP:UniProtKB.
DR GO; GO:1990261; P:pre-mRNA catabolic process; IGI:UniProtKB.
DR GO; GO:0051726; P:regulation of cell cycle; IEA:InterPro.
DR GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:2000036; P:regulation of stem cell population maintenance; IGI:UniProtKB.
DR InterPro; IPR014720; dsRBD_dom.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR032922; SON.
DR PANTHER; PTHR46528; PTHR46528; 1.
DR Pfam; PF00035; dsrm; 1.
DR Pfam; PF01585; G-patch; 1.
DR SMART; SM00358; DSRM; 1.
DR SMART; SM00443; G_patch; 1.
DR PROSITE; PS50137; DS_RBD; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..874
FT /note="Protein Son"
FT /evidence="ECO:0000305"
FT /id="PRO_0000449793"
FT DOMAIN 705..751
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT DOMAIN 800..870
FT /note="DRBM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00266"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 68..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 120..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..84
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..145
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 146..162
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..278
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 308..352
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 21
FT /note="T -> P (in Ref. 3; AAN71087)"
FT /evidence="ECO:0000305"
FT CONFLICT 33
FT /note="K -> E (in Ref. 3; AAN71087)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="E -> D (in Ref. 3; AAN71087)"
FT /evidence="ECO:0000305"
FT CONFLICT 179
FT /note="D -> DKEKDRHRDRDKS (in Ref. 3; AAN71087)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="R -> Q (in Ref. 3; AAN71087)"
FT /evidence="ECO:0000305"
FT CONFLICT 549
FT /note="Q -> K (in Ref. 3; AAM50693)"
FT /evidence="ECO:0000305"
FT CONFLICT 789..791
FT /note="LAS -> VAP (in Ref. 3; AAM50693)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 874 AA; 97760 MW; E92458F78297A379 CRC64;
MTENTEKGAS VETPQVAGSQ TNPPVQEPLA LTKIPPIKVK SERPDAEVEA KLRAMNAKIK
AEMVTLMRRS NSNELGNNDE SGESESSASA DDKKNIKPVK SSNEILAELF GVFNAAPPEE
LLDDNLFKKK KKVKKEKKDK KAKKKKTTKS DGECSDSEAE GKHKHKRKKH KHKDIRVKDK
EKDRDRDKSK EKDRDRVTDK SKEKDRDRDR DRDKSKDKFT AAQAPSEKEK EKSESRKRSA
VEPSSHSEKR ERHEREKHRD WEREREREKE HERERVRSNN SFYNGQREAD RLKGSESAST
KSRQEQDLSD ISLSDEESYL REKASNGRRR AHNSFYDEKE ELSVSPKRNV RESNTRRNRK
SRSRSRDLGI DKKRLLEIAR RNAINMFKQG TMPGVANMTA EVKDKVLVKM RYGGRTIQDL
TDFCKKISNG DGLSDLSSEE ESDVDKNGNA KVFHHPFQLK EREPIVMHIR NSTALVPAPP
RLDEQTKAIT MQFPVSSGQT HRNNEVWVPV DPKDSLVPLP SLPPAKQATN MFKETPKNVF
AKSIPLQEQQ EPAFKPLGGA VVVPPLAATQ LPTVPQSVPP TVPKEFAPPA VPFVPEVPIP
STSPVTPMQS ASIFPDVTPP SMDVSSIITQ RLSAIRRLQE NPADSEALKM MYTAQRNMSS
WANSKHLPGQ FTGSTGAQVM KAHELNSGPQ LWVRKDQMTS TKPVTGGMGM ALLQKMGWKP
GEGLGRCKTG SLQPLLLDVK LDKRGLVSRD DLRPPQMRAP AAQRRNKNMA GPIGAGPCPA
VQGAGPGPLA STPLVTQDKH PVCVLNELTS KNKWMPPQYK LRQDIGPAHN RSFLFSVEIN
GQTFTPDRGS NNKKEAKLNA AALCLRSLGI LPPS
