SOPB_SALTY
ID SOPB_SALTY Reviewed; 561 AA.
AC O30916; Q8ZQ57; Q9L889;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Inositol phosphate phosphatase SopB;
DE EC=3.1.3.-;
DE AltName: Full=Effector protein SopB;
GN Name=sopB; Synonyms=sigD; OrderedLocusNames=STM1091;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=9537377; DOI=10.1128/jb.180.7.1793-1802.1998;
RA Hong K.H., Miller V.L.;
RT "Identification of a novel Salmonella invasion locus homologous to Shigella
RT ipgDE.";
RL J. Bacteriol. 180:1793-1802(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 97-529.
RC STRAIN=IE1534;
RX PubMed=11200542; DOI=10.1016/s1438-4221(00)80009-0;
RA Prager R., Mirold S., Tietze E., Strutz U., Knuppel B., Rabsch W.,
RA Hardt W.-D., Tschape H.;
RT "Prevalence and polymorphism of genes encoding translocated effector
RT proteins among clinical isolates of Salmonella enterica.";
RL Int. J. Med. Microbiol. 290:605-617(2000).
RN [4]
RP ROLE IN HOST CELL INVASION, AND SUBCELLULAR LOCATION.
RC STRAIN=SL1344;
RX PubMed=11244077; DOI=10.1128/jb.183.7.2348-2358.2001;
RA Mirold S., Ehrbar K., Weissmueller A., Prager R., Tschaepe H.,
RA Ruessmann H., Hardt W.-D.;
RT "Salmonella host cell invasion emerged by acquisition of a mosaic of
RT separate genetic elements, including Salmonella pathogenicity island 1
RT (SPI1), SPI5, and sopE2.";
RL J. Bacteriol. 183:2348-2358(2001).
RN [5]
RP FUNCTION.
RX PubMed=11136447; DOI=10.1046/j.1365-2958.2001.02230.x;
RA Zhou D., Chen L.-M., Hernandez L.D., Shears S.B., Galan J.E.;
RT "A Salmonella inositol polyphosphatase acts in conjunction with other
RT bacterial effectors to promote host cell actin cytoskeleton rearrangements
RT and bacterial internalization.";
RL Mol. Microbiol. 39:248-259(2001).
RN [6]
RP FUNCTION.
RC STRAIN=SLI344;
RX PubMed=12360287; DOI=10.1038/ncb854;
RA Terebiznik M.R., Vieira O.V., Marcus S.L., Slade A., Yip C.M.,
RA Trimble W.S., Meyer T., Finlay B.B., Grinstein S.;
RT "Elimination of host cell PtdIns(4,5)P(2) by bacterial SigD promotes
RT membrane fission during invasion by Salmonella.";
RL Nat. Cell Biol. 4:766-773(2002).
RN [7]
RP FUNCTION.
RX PubMed=15205533; DOI=10.1126/science.1098188;
RA Hernandez L.D., Hueffer K., Wenk M.R., Galan J.E.;
RT "Salmonella modulates vesicular traffic by altering phosphoinositide
RT metabolism.";
RL Science 304:1805-1807(2004).
RN [8]
RP MUTAGENESIS OF CYS-460; LYS-525 AND LYS-528.
RX PubMed=11311241; DOI=10.1016/s0014-5793(01)02356-0;
RA Marcus S.L., Wenk M.R., Steele-Mortimer O., Finlay B.B.;
RT "A synaptojanin-homologous region of Salmonella typhimurium SigD is
RT essential for inositol phosphatase activity and Akt activation.";
RL FEBS Lett. 494:201-207(2001).
CC -!- FUNCTION: Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns
CC 3,4,5-P3) to PtdIns 3-P and prevents the transition of PtdIns 3-P to
CC PtdIns 3,5-P2. It is one of the known effectors injected by Salmonella
CC into the host cell and is required for invasion and for an efficient
CC generation and maintenance of Salmonella-containing vacuole (SVC).
CC Alteration of the phosphoinositide composition of the plasma membrane
CC causes membrane ruffling and actin cytoskeleton rearrangements. The
CC persistence of PtdIns 3-P diverts the SCV from the endocytic pathway
CC resulting in enlarged vesicles, which are essential to create a
CC favorable environment where Salmonella can replicate and avoid immune
CC defenses of the host cell. {ECO:0000269|PubMed:11136447,
CC ECO:0000269|PubMed:11244077, ECO:0000269|PubMed:12360287,
CC ECO:0000269|PubMed:15205533}.
CC -!- INTERACTION:
CC O30916; P60953: CDC42; Xeno; NbExp=5; IntAct=EBI-11167349, EBI-81752;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Secreted via the
CC type III secretion system 1 (SPI-1 TTSS). {ECO:0000250}.
CC -!- DOMAIN: Contains the consensus sequence Cys-X(5)-Arg characteristic of
CC Mg-independent phosphatases.
CC -!- SIMILARITY: Belongs to the phosphatase IpgD/SopB family. {ECO:0000305}.
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DR EMBL; AF021817; AAC46234.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20023.1; -; Genomic_DNA.
DR EMBL; AF213335; AAF21057.2; -; Genomic_DNA.
DR RefSeq; NP_460064.1; NC_003197.2.
DR RefSeq; WP_001166946.1; NC_003197.2.
DR PDB; 4DID; X-ray; 2.35 A; B=30-181.
DR PDBsum; 4DID; -.
DR AlphaFoldDB; O30916; -.
DR SMR; O30916; -.
DR IntAct; O30916; 1.
DR STRING; 99287.STM1091; -.
DR PaxDb; O30916; -.
DR EnsemblBacteria; AAL20023; AAL20023; STM1091.
DR GeneID; 1252609; -.
DR KEGG; stm:STM1091; -.
DR PATRIC; fig|99287.12.peg.1155; -.
DR HOGENOM; CLU_025781_0_0_6; -.
DR OMA; CWNCKSG; -.
DR BioCyc; MetaCyc:MON-15186; -.
DR BioCyc; SENT99287:STM1091-MON; -.
DR BRENDA; 3.1.3.78; 5542.
DR PHI-base; PHI:6738; -.
DR PHI-base; PHI:7236; -.
DR PHI-base; PHI:7920; -.
DR PHI-base; PHI:8298; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0071944; C:cell periphery; IMP:AgBase.
DR GO; GO:0020003; C:symbiont-containing vacuole; IDA:AgBase.
DR GO; GO:0042577; F:lipid phosphatase activity; IMP:AgBase.
DR GO; GO:0030308; P:negative regulation of cell growth; IMP:AgBase.
DR GO; GO:0023057; P:negative regulation of signaling; IMP:AgBase.
DR GO; GO:0045740; P:positive regulation of DNA replication; IDA:AgBase.
DR GO; GO:0045859; P:regulation of protein kinase activity; IMP:AgBase.
DR DisProt; DP01166; -.
DR InterPro; IPR008108; IpgD/SopB.
DR Pfam; PF05925; IpgD; 1.
DR PRINTS; PR01734; TYPE3OMBPROT.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome; Secreted; Virulence.
FT CHAIN 1..561
FT /note="Inositol phosphate phosphatase SopB"
FT /id="PRO_0000220495"
FT MOTIF 460..466
FT /note="CX5R motif"
FT ACT_SITE 460
FT /evidence="ECO:0000255"
FT MUTAGEN 460
FT /note="C->S: Loss of activity."
FT /evidence="ECO:0000269|PubMed:11311241"
FT MUTAGEN 525
FT /note="K->A: Decrease in activity."
FT /evidence="ECO:0000269|PubMed:11311241"
FT MUTAGEN 528
FT /note="K->A: Loss of activity."
FT /evidence="ECO:0000269|PubMed:11311241"
FT CONFLICT 244
FT /note="C -> FD (in Ref. 1; AAC46234)"
FT /evidence="ECO:0000305"
FT CONFLICT 354
FT /note="V -> VV (in Ref. 1; AAC46234)"
FT /evidence="ECO:0000305"
FT CONFLICT 476
FT /note="R -> G (in Ref. 1; AAC46234)"
FT /evidence="ECO:0000305"
FT STRAND 49..52
FT /evidence="ECO:0007829|PDB:4DID"
FT HELIX 59..62
FT /evidence="ECO:0007829|PDB:4DID"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:4DID"
FT HELIX 73..90
FT /evidence="ECO:0007829|PDB:4DID"
FT TURN 91..93
FT /evidence="ECO:0007829|PDB:4DID"
FT HELIX 96..107
FT /evidence="ECO:0007829|PDB:4DID"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:4DID"
FT HELIX 118..142
FT /evidence="ECO:0007829|PDB:4DID"
FT HELIX 147..166
FT /evidence="ECO:0007829|PDB:4DID"
SQ SEQUENCE 561 AA; 61935 MW; A484B55502CFA643 CRC64;
MQIQSFYHSA SLKTQEAFKS LQKTLYNGMQ ILSGQGKAPA KAPDARPEII VLREPGATWG
NYLQHQKASN HSLHNLYNLQ RDLLTVAATV LGKQDPVLTS MANQMELAKV KADRPATKQE
EAAAKALKKN LIELIAARTQ QQDGLPAKEA HRFAAVAFRD AQVKQLNNQP WQTIKNTLTH
NGHHYTNTQL PAAEMKIGAK DIFPSAYEGK GVCSWDTKNI HHANNLWMST VSVHEDGKDK
TLFCGIRHGV LSPYHEKDPL LRHVGAENKA KEVLTAALFS KPELLNKALA GEAVSLKLVS
VGLLTASNIF GKEGTMVEDQ MRAWQSLTQP GKMIHLKIRN KDGDLQTVKI KPDVAAFNVG
VNELALKLGF GLKASDSYNA EALHQLLGND LRPEARPGGW VGEWLAQYPD NYEVVNTLAR
QIKDIWKNNQ HHKDGGEPYK LAQRLAMLAH EIDAVPAWNC KSGKDRTGMM DSEIKREIIS
LHQTHMLSAP GSLPDSGGQK IFQKVLLNSG NLEIQKQNTG GAGNKVMKNL SPEVLNLSYQ
KRVGDENIWQ SVKGISSLIT S
