ID   SOPT_PLAFO              Reviewed;         893 AA.
AC   A0A2I0BUK1;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2018, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Inactive subtilisin-like protease SOPT {ECO:0000305};
DE   AltName: Full=Subtilisin-like ookinete protein important for transmission {ECO:0000303|PubMed:29873127};
GN   Name=SOPT {ECO:0000303|PubMed:29873127};
GN   ORFNames=CK202_3559 {ECO:0000312|EMBL:PKC45874.1};
OS   Plasmodium falciparum (isolate NF54).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX   NCBI_TaxID=5843 {ECO:0000312|Proteomes:UP000232684};
RN   [1] {ECO:0000312|Proteomes:UP000232684}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NF54 {ECO:0000312|Proteomes:UP000232684};
RA   Bryant J.M., Baumgarten S., Scheidig-Benatar C., Scherf A.;
RT   "Plasmodium falciparum NF54 genome assembly.";
RL   Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=29873127; DOI=10.1111/mmi.13993;
RA   Armistead J.S., Jennison C., O'Neill M.T., Lopaticki S., Liehl P.,
RA   Hanson K.K., Annoura T., Rajasekaran P., Erickson S.M., Tonkin C.J.,
RA   Khan S.M., Mota M.M., Boddey J.A.;
RT   "Plasmodium falciparum subtilisin-like ookinete protein SOPT plays an
RT   important and conserved role during ookinete infection of the Anopheles
RT   stephensi midgut.";
RL   Mol. Microbiol. 109:458-473(2018).
CC   -!- FUNCTION: Probable inactive serine protease which plays a role in
CC       ookinete traversal of the mosquito host midgut epithelium.
CC       {ECO:0000269|PubMed:29873127}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:29873127}.
CC       Note=Predominantly, localizes in the anterior part of the ookinete
CC       (PubMed:29873127). May localize to the secretory compartments
CC       (PubMed:29873127). {ECO:0000269|PubMed:29873127,
CC       ECO:0000303|PubMed:29873127}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in ookinetes (at protein level).
CC       {ECO:0000269|PubMed:29873127}.
CC   -!- DISRUPTION PHENOTYPE: Ookinetes fail to traverse the midgut epithelium
CC       resulting in a reduced number of oocysts in the gut epithelium of the
CC       mosquito host A.stephensi (PubMed:29873127). Gametogenesis and ookinete
CC       development are normal (PubMed:29873127). Growth rate and development
CC       of blood stage parasites are normal (PubMed:29873127).
CC       {ECO:0000269|PubMed:29873127}.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
CC   -!- CAUTION: In contrast to other members of the peptidase S8 family,
CC       contains Gly and Glu residues at the position of the canonical
CC       catalytic Asp and His, respectively, suggesting that the protein lacks
CC       protease activity. {ECO:0000305}.
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DR   EMBL; NYMT01000012; PKC45874.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2I0BUK1; -.
DR   VEuPathDB; PlasmoDB:PfNF54_050011600; -.
DR   Proteomes; UP000232684; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane.
FT   CHAIN           1..893
FT                   /note="Inactive subtilisin-like protease SOPT"
FT                   /id="PRO_0000451408"
FT   REGION          787..893
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        787..804
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        805..820
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        831..852
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        853..868
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   893 AA;  107039 MW;  14DEEF47CFA85A05 CRC64;
     MIYLGKKLLS CTLFVYFLYI HFFLLKQNNF CDVKVRERIL EESINNDLSS KGENLHIYEK
     TNVSVQTFIK KKERKNLSDN NINDKINNNN NYNNNNNNNI EDTTYYPTGN EKKENIFLKI
     FRYVKNWFPI KSSNNLKKTN INYEQVQEEN EFSKYILQNN MSIETTKVCL IGSGGDSSHD
     LIKQFLLHNN VKYNRNYEND NSLNNKSGSI ISSYKYYSEE IIQEPYIIDR DICNKKKNCK
     ESTLNYENNP KTLIGNIIIQ SDILKNEKIF NMNRHFVVCK YFGSIPKTNI KINQSTLIQH
     LIKCLDYCKM EGVQYIYIGY NIYAANNKLI EIMKKLREHK IIIVTSSGKI YDDDNNNDNN
     NFYNDNIYNN NIYNVHNDDK EKIKNHIKKK NKQNNYLYEY QRTQKDEEQK SNSSLYQNLE
     NVISISGLIY TDSSKKNKNK NYIYDNEIKI LDQKGNKKLN RNYISLFYFS YDTDIYEKIE
     SDIIDDDHDL VSASFVNTLV LMHSINLKLS LGRLRKILNK SIVKREELRH LSNRAYYHDM
     MNTFEDSLNQ RKRSYKIFYL ELKNNKHKVL LSDANLKSMY QDNLPVNYNE EDHVKHNVEQ
     ETSVERDIYK NNENSNKNRK MDMDEGKGTY IQNKESHKYN IHYPYNRIKQ SLLNDNTLNH
     KPYVSFLNMS YYNEDIEKRN YNIYDDPSYT YDQGITYDDN YYIDDHDIHT RKKRKISYDG
     EDNNDYHMYD DRDNLFHSNL GNNKYEDDGN VHREKEKDLE PRFLYDPFAN IENRDLETVQ
     ELSELREKKS NNFYSRNHDN SSNMKRRRKE KKKKLKKVLR SKYDKIGNLE RIRRKKKRRM
     IHKNKINKRR NMKRKNNELE ERRNKQADKN SSSGNGKGKI NGTRNSPKIK FKR