ID   SOT4_ARATH              Reviewed;         273 AA.
AC   Q8RUC1;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Cytosolic sulfotransferase 4;
DE            Short=AtSOT4;
DE            EC=2.8.2.-;
GN   Name=SOT4; OrderedLocusNames=At2g27570; ORFNames=F10A12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15234990; DOI=10.1093/jxb/erh183;
RA   Klein M., Papenbrock J.;
RT   "The multi-protein family of Arabidopsis sulphotransferases and their
RT   relatives in other plant species.";
RL   J. Exp. Bot. 55:1809-1820(2004).
CC   -!- FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate
CC       (PAPS) as sulfonate donor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sulfotransferase 1 family. {ECO:0000305}.
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DR   EMBL; AC005824; AAM15079.1; -; Genomic_DNA.
DR   EMBL; AC006232; AAM15179.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08015.1; -; Genomic_DNA.
DR   RefSeq; NP_180325.1; NM_128316.1.
DR   AlphaFoldDB; Q8RUC1; -.
DR   SMR; Q8RUC1; -.
DR   PaxDb; Q8RUC1; -.
DR   PRIDE; Q8RUC1; -.
DR   ProteomicsDB; 232603; -.
DR   EnsemblPlants; AT2G27570.1; AT2G27570.1; AT2G27570.
DR   GeneID; 817303; -.
DR   Gramene; AT2G27570.1; AT2G27570.1; AT2G27570.
DR   KEGG; ath:AT2G27570; -.
DR   Araport; AT2G27570; -.
DR   TAIR; locus:2042026; AT2G27570.
DR   eggNOG; KOG1584; Eukaryota.
DR   HOGENOM; CLU_027239_0_3_1; -.
DR   OMA; PMFESLC; -.
DR   OrthoDB; 780670at2759; -.
DR   PhylomeDB; Q8RUC1; -.
DR   BioCyc; ARA:AT2G27570-MON; -.
DR   PRO; PR:Q8RUC1; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q8RUC1; baseline and differential.
DR   Genevisible; Q8RUC1; AT.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR   GO; GO:0051923; P:sulfation; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000863; Sulfotransferase_dom.
DR   Pfam; PF00685; Sulfotransfer_1; 3.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Transferase.
FT   CHAIN           1..273
FT                   /note="Cytosolic sulfotransferase 4"
FT                   /id="PRO_0000417052"
FT   ACT_SITE        121
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         74..79
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
FT   BINDING         239..241
FT                   /ligand="3'-phosphoadenylyl sulfate"
FT                   /ligand_id="ChEBI:CHEBI:58339"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   273 AA;  31933 MW;  E2E20AA4D0C965F6 CRC64;
     MDDKKMAVNL RNDDLSEETK TLISSLPSDK DSTGINVCKY QGCWYTHHFL QAVLNFQKNF
     KPQDTNIIVA SFPKCGTTWL KALTFSLVHR SKHPSHDHHH PLLSNNPHVL FSSSPRLFST
     HMPSHTLQEV LKDSTCKVVY ICRNMKDTLE TFESFCKGVN FFGPFWDQVL SYWRRSLEDP
     NHVLFMRFEE MKAEPHEQIK RLAEFLDSPL LRKKKRTDCL EINKTGKLCE GRDNKTFFRK
     GEVGDWKNYL TPEMENKIDM IIQEKLQNSG LKF