SOT8_ARATH
ID SOT8_ARATH Reviewed; 331 AA.
AC Q9FX56;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Cytosolic sulfotransferase 8;
DE Short=AtSOT8;
DE EC=2.8.2.-;
DE AltName: Full=Sulfotransferase 4b;
DE Short=AtST4b;
GN Name=SOT8; Synonyms=ST4B; OrderedLocusNames=At1g13420; ORFNames=T6J4.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15234990; DOI=10.1093/jxb/erh183;
RA Klein M., Papenbrock J.;
RT "The multi-protein family of Arabidopsis sulphotransferases and their
RT relatives in other plant species.";
RL J. Exp. Bot. 55:1809-1820(2004).
RN [5]
RP FUNCTION, INDUCTION BY TRANS-ZEATIN, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=17039368; DOI=10.1007/s00425-006-0413-y;
RA Marsolais F., Boyd J., Paredes Y., Schinas A.M., Garcia M., Elzein S.,
RA Varin L.;
RT "Molecular and biochemical characterization of two brassinosteroid
RT sulfotransferases from Arabidopsis, AtST4a (At2g14920) and AtST1
RT (At2g03760).";
RL Planta 225:1233-1244(2007).
CC -!- FUNCTION: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate
CC (PAPS) as sulfonate donor. No activity with brassinosteroids.
CC {ECO:0000269|PubMed:17039368}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings and roots.
CC {ECO:0000269|PubMed:17039368}.
CC -!- INDUCTION: Up-regulated by trans-zeatin. {ECO:0000269|PubMed:17039368}.
CC -!- SIMILARITY: Belongs to the sulfotransferase 1 family. {ECO:0000305}.
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DR EMBL; AC011810; AAG09547.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29014.1; -; Genomic_DNA.
DR EMBL; BT030329; ABO09892.1; -; mRNA.
DR RefSeq; NP_172799.1; NM_101212.3.
DR AlphaFoldDB; Q9FX56; -.
DR SMR; Q9FX56; -.
DR STRING; 3702.AT1G13420.1; -.
DR iPTMnet; Q9FX56; -.
DR PaxDb; Q9FX56; -.
DR PRIDE; Q9FX56; -.
DR ProteomicsDB; 245329; -.
DR EnsemblPlants; AT1G13420.1; AT1G13420.1; AT1G13420.
DR GeneID; 837902; -.
DR Gramene; AT1G13420.1; AT1G13420.1; AT1G13420.
DR KEGG; ath:AT1G13420; -.
DR Araport; AT1G13420; -.
DR TAIR; locus:2009992; AT1G13420.
DR eggNOG; KOG1584; Eukaryota.
DR HOGENOM; CLU_027239_0_3_1; -.
DR InParanoid; Q9FX56; -.
DR OMA; HYEPREN; -.
DR OrthoDB; 780670at2759; -.
DR PhylomeDB; Q9FX56; -.
DR BioCyc; ARA:AT1G13420-MON; -.
DR PRO; PR:Q9FX56; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FX56; baseline and differential.
DR Genevisible; Q9FX56; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0008146; F:sulfotransferase activity; IBA:GO_Central.
DR GO; GO:0009735; P:response to cytokinin; IEP:TAIR.
DR GO; GO:0051923; P:sulfation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Transferase.
FT CHAIN 1..331
FT /note="Cytosolic sulfotransferase 8"
FT /id="PRO_0000417056"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 145
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 80..85
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
FT BINDING 167
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
FT BINDING 175
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
FT BINDING 231
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
FT BINDING 297..299
FT /ligand="3'-phosphoadenylyl sulfate"
FT /ligand_id="ChEBI:CHEBI:58339"
FT /evidence="ECO:0000250"
SQ SEQUENCE 331 AA; 37709 MW; 45DC8565C780E324 CRC64;
MGEKDIPRNL KEEEEEEEEN QSEETKSLIS SLPSDIDCSG TKLYKYQGCW YDKDILQAIL
NFNKNFQPQE TDIIVASFPK SGTTWLKALT FALAQRSKHT SDNHPLLTHN PHELVPYLEL
DLYLKSSKPD LTKLPSSSPR LFSTHMSFDA LKVPLKESPC KIVYVCRNVK DVLVSLWCFE
NSMSGENNLS LEALFESLCS GVNLCGPLWE NVLGYWRGSL EDPKHVLFLR YEELKTEPRV
QIKRLAEFLD CPFTKEEEDS GGVDKILELC SLRNLSGLEI NKTGSLSEGV SFKSFFRKGE
VGDWKSYMTP EMENKIDMIV EEKLQGSGLK L
