SOTB_DICCH
ID SOTB_DICCH Reviewed; 395 AA.
AC Q9S3J9;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Sugar efflux transporter B;
GN Name=sotB;
OS Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Dickeya.
OX NCBI_TaxID=556;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, FUNCTION, SUBCELLULAR
RP LOCATION, AND INDUCTION.
RC STRAIN=A350;
RX PubMed=10671456; DOI=10.1128/jb.182.5.1340-1345.2000;
RA Condemine G.;
RT "Characterization of SotA and SotB, two Erwinia chrysanthemi proteins which
RT modify isopropyl-beta-D-thiogalactopyranoside and lactose induction of the
RT Escherichia coli lac promoter.";
RL J. Bacteriol. 182:1340-1345(2000).
CC -!- FUNCTION: Involved in the efflux of sugars. The physiological role may
CC be the reduction of the intracellular concentration of toxic sugars or
CC sugar metabolites. Transports IPTG, lactose and melibiose.
CC {ECO:0000269|PubMed:10671456}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:10671456}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:10671456}.
CC -!- INDUCTION: Activated by glucose, and to a lesser extent by other
CC metabolizable sugars such as galacturonate, arabinose and melibiose.
CC Repressed by cyclic AMP receptor protein (CRP).
CC {ECO:0000269|PubMed:10671456}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. SotB (TC
CC 2.A.1.2) family. {ECO:0000305}.
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DR EMBL; AJ249181; CAB53453.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9S3J9; -.
DR SMR; Q9S3J9; -.
DR TCDB; 2.A.1.2.18; the major facilitator superfamily (mfs).
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015144; F:carbohydrate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1250.20; -; 1.
DR HAMAP; MF_00517; MFS_SotB; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR023495; Sugar_effux_transptr_put.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..395
FT /note="Sugar efflux transporter B"
FT /id="PRO_0000209326"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 395 AA; 41954 MW; 759EA39D0CAF43F5 CRC64;
MTSASPSRST AWLRVVTLAI AAFIFNTTEF IPVGLLSDIA NSFAMKTEDV GLMITIYAWI
VAVASLICML LTSGIERRKL LIGLFSLFIL SHLLSAVAWN FTVLVISRAG VALAHSVFWS
ITASLAIRMA PPGKRAQALG LIATGSSLAM VLGLPLGRVI GQYLGWRVTF LTIAAGATVA
MILLARLLPL LPSEHSGSLG SVPKLFRRPA LVGIYLLTVV VVTAHFTAYS YIEPFIQTVA
GLPENFTTLI LLLFGCAGIA GSMLYSRYSD RFPIGFLVTA MLLLLACLTL LMPLSGYPFG
LTLLCLVWGL AMMSIGLAMQ AKVLSLAPDA SDVAMSIFSG LFNLGIGGGA LLGSQVSLHL
GMDKIGYVGA PLVLVALFAT LLSVYRSVRL VHSRV