ID   SOTB_ECODH              Reviewed;         396 AA.
AC   B1XEB5;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Probable sugar efflux transporter {ECO:0000255|HAMAP-Rule:MF_00517};
GN   Name=sotB {ECO:0000255|HAMAP-Rule:MF_00517};
GN   OrderedLocusNames=ECDH10B_1659;
OS   Escherichia coli (strain K12 / DH10B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / DH10B;
RX   PubMed=18245285; DOI=10.1128/jb.01695-07;
RA   Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA   Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA   Posfai G., Weinstock G.M., Blattner F.R.;
RT   "The complete genome sequence of Escherichia coli DH10B: insights into the
RT   biology of a laboratory workhorse.";
RL   J. Bacteriol. 190:2597-2606(2008).
CC   -!- FUNCTION: Involved in the efflux of sugars. The physiological role may
CC       be the reduction of the intracellular concentration of toxic sugars or
CC       sugar metabolites. {ECO:0000255|HAMAP-Rule:MF_00517}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00517}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00517}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. SotB (TC
CC       2.A.1.2) family. {ECO:0000255|HAMAP-Rule:MF_00517}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000948; ACB02737.1; -; Genomic_DNA.
DR   RefSeq; WP_000210799.1; NC_010473.1.
DR   AlphaFoldDB; B1XEB5; -.
DR   SMR; B1XEB5; -.
DR   KEGG; ecd:ECDH10B_1659; -.
DR   HOGENOM; CLU_001265_61_1_6; -.
DR   OMA; SAHFTAY; -.
DR   BioCyc; ECOL316385:ECDH10B_RS08500-MON; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015144; F:carbohydrate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   HAMAP; MF_00517; MFS_SotB; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR023495; Sugar_effux_transptr_put.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..396
FT                   /note="Probable sugar efflux transporter"
FT                   /id="PRO_1000127458"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        275..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00517"
SQ   SEQUENCE   396 AA;  42538 MW;  CB6A34CA4EE6D4F0 CRC64;
     MTTNTVSRKV AWLRVVTLAV AAFIFNTTEF VPVGLLSDIA QSFHMQTAQV GIMLTIYAWV
     VALMSLPFML MTSQVERRKL LICLFVVFIA SHVLSFLSWS FTVLVISRIG VAFAHAIFWS
     ITASLAIRMA PAGKRAQALS LIATGTALAM VLGLPLGRIV GQYFGWRMTF FAIGIGALIT
     LLCLIKLLPL LPSEHSGSLK SLPLLFRRPA LMSIYLLTVV VVTAHYTAYS YIEPFVQNIA
     GFSANFATAL LLLLGGAGII GSVIFGKLGN QYASALVSTA IALLLVCLAL LLPAANSEIH
     LGVLSIFWGI AMMIIGLGMQ VKVLALAPDA TDVAMALFSG IFNIGIGAGA LVGNQVSLHW
     SMSMIGYVGA VPAFAALIWS IIIFRRWPVT LEEQTQ