SOX2_CAEEL
ID SOX2_CAEEL Reviewed; 283 AA.
AC Q21305;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Transcription factor sox-2 {ECO:0000305};
GN Name=sox-2 {ECO:0000312|WormBase:K08A8.2a};
GN ORFNames=K08A8.2 {ECO:0000312|WormBase:K08A8.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, INTERACTION WITH EGL-27 AND CEH-6, AND DISRUPTION PHENOTYPE.
RX PubMed=22493276; DOI=10.1073/pnas.1117031109;
RA Kagias K., Ahier A., Fischer N., Jarriault S.;
RT "Members of the NODE (Nanog and Oct4-associated deacetylase) complex and
RT SOX-2 promote the initiation of a natural cellular reprogramming event in
RT vivo.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:6596-6601(2012).
RN [3]
RP INTERACTION WITH WDR-5.1.
RX PubMed=25124442; DOI=10.1126/science.1255885;
RA Zuryn S., Ahier A., Portoso M., White E.R., Morin M.C., Margueron R.,
RA Jarriault S.;
RT "Sequential histone-modifying activities determine the robustness of
RT transdifferentiation.";
RL Science 345:826-829(2014).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26153233; DOI=10.1242/dev.125740;
RA Vidal B., Santella A., Serrano-Saiz E., Bao Z., Chuang C.F., Hobert O.;
RT "C. elegans SoxB genes are dispensable for embryonic neurogenesis but
RT required for terminal differentiation of specific neuron types.";
RL Development 142:2464-2477(2015).
RN [5]
RP FUNCTION, INTERACTION WITH CEH-36 AND LIM-4, TISSUE SPECIFICITY, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF GLY-73.
RX PubMed=26341465; DOI=10.15252/embj.201592188;
RA Alqadah A., Hsieh Y.W., Vidal B., Chang C., Hobert O., Chuang C.F.;
RT "Postmitotic diversification of olfactory neuron types is mediated by
RT differential activities of the HMG-box transcription factor SOX-2.";
RL EMBO J. 34:2574-2589(2015).
CC -!- FUNCTION: Probable transcription factor that regulates the lineage
CC progression of embryonic blast cells and controls the postmitotic
CC specification and differentiation of neurons (PubMed:26153233,
CC PubMed:26341465). Cooperates with additional factors to direct the
CC differentiation of the olfactory neurons, functioning with the
CC transcription factor ceh-36 to specify AWC neurons and with the LIM
CC homeodomain factor lim-4 to suppress AWC terminal differentiation and
CC promote AWB neuron differentiation (PubMed:26341465). Plays a role in
CC the terminal differentiation of glutamatergic and cholinergic neurons
CC (PubMed:26153233). Required for natural reprogramming events and in
CC particular for the transdifferentiation of the Y rectal epithelial cell
CC to the PDA motor neuron during larval development (PubMed:22493276).
CC {ECO:0000269|PubMed:22493276, ECO:0000269|PubMed:26153233,
CC ECO:0000269|PubMed:26341465}.
CC -!- SUBUNIT: Interacts with egl-27 and ceh-6 (PubMed:22493276). Interacts
CC with ceh-36 and lim-4 (PubMed:26341465). Interacts with wdr-5.1
CC (PubMed:25124442). {ECO:0000269|PubMed:22493276,
CC ECO:0000269|PubMed:25124442, ECO:0000269|PubMed:26341465}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}.
CC -!- TISSUE SPECIFICITY: Expressed in AWC and AWB olfactory neurons
CC throughout life (PubMed:26153233, PubMed:26341465). Also expressed in
CC the IL1, IL2, URA, URB and OLL sensory neurons, the AIM, AIN, AVK, RIH
CC interneurons, and the motor neuron class RME (PubMed:26153233).
CC {ECO:0000269|PubMed:26153233, ECO:0000269|PubMed:26341465}.
CC -!- DEVELOPMENTAL STAGE: Limited expression during embryogenesis with
CC expression in only a subset of neuroblasts. Expressed in neuronal
CC progenitor cells late in nervous system development and in some non-
CC neural progenitor cells. Expressed broadly during larval development
CC stages. {ECO:0000269|PubMed:26153233}.
CC -!- DISRUPTION PHENOTYPE: Animals arrest at the first larval stage of
CC development with an unattached pharynx (PubMed:26153233). Able to form
CC neurons, but with defective terminal differentiation of both AWC and
CC AWB olfactory neurons, OLL and IL1 sensory glutamatergic neurons and
CC cholinergic neurons including IL2, URA and URB (PubMed:26153233,
CC PubMed:26341465). RNAi-mediated knockdown results in failed
CC transdifferentiation of the Y rectal epithelial cell to the PDA motor
CC neuron (PubMed:22493276). {ECO:0000269|PubMed:22493276,
CC ECO:0000269|PubMed:26153233, ECO:0000269|PubMed:26341465}.
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DR EMBL; BX284606; CCD62389.1; -; Genomic_DNA.
DR PIR; T16581; T16581.
DR RefSeq; NP_741836.1; NM_171725.4.
DR AlphaFoldDB; Q21305; -.
DR SMR; Q21305; -.
DR DIP; DIP-61431N; -.
DR IntAct; Q21305; 3.
DR STRING; 6239.K08A8.2a.1; -.
DR EPD; Q21305; -.
DR PaxDb; Q21305; -.
DR PeptideAtlas; Q21305; -.
DR EnsemblMetazoa; K08A8.2a.1; K08A8.2a.1; WBGene00004949.
DR EnsemblMetazoa; K08A8.2a.2; K08A8.2a.2; WBGene00004949.
DR GeneID; 181002; -.
DR KEGG; cel:CELE_K08A8.2; -.
DR UCSC; K08A8.2a.1; c. elegans.
DR CTD; 181002; -.
DR WormBase; K08A8.2a; CE28595; WBGene00004949; sox-2.
DR eggNOG; KOG0527; Eukaryota.
DR InParanoid; Q21305; -.
DR OMA; MVWSRSK; -.
DR OrthoDB; 1255488at2759; -.
DR PhylomeDB; Q21305; -.
DR Reactome; R-CEL-3769402; Deactivation of the beta-catenin transactivating complex.
DR PRO; PR:Q21305; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00004949; Expressed in nervous system and 72 other tissues.
DR ExpressionAtlas; Q21305; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0048665; P:neuron fate specification; IMP:WormBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Differentiation; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..283
FT /note="Transcription factor sox-2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436608"
FT DNA_BIND 60..128
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 24..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 73
FT /note="G->E: In ky707; reduced association with lim-4
FT resulting in AWB olfactory neurons adopting an AWC
FT olfactory neuron cell identity."
FT /evidence="ECO:0000269|PubMed:26341465"
SQ SEQUENCE 283 AA; 32169 MW; 71F46FD3BDDAAFA4 CRC64;
MMMDPDSAKM QDYSGWSFGF HMPPTSSTNL LPQSMPDDLS NGPDSPDSTG KDGKKNDDRV
KRPMNAFMVW SRGQRKKMAL ENPKMHNSEI SKRLGTEWKM LSEQEKRPFI DEAKRLRAIH
MKEHPDYKYR PRRKTKSINK KNGAPIPFGN LDTKTPSYPT LTTNWNATNQ YIDQFRFAPY
PTTTVMDQIP FSLTYPVHSV PTDNSSPSQF QPSPMSTNFA GSYLTPKSES SPVGSDSTVG
TVDSSQFRAY YDHTKDQMMY PYSIELTHAQ NIQNALSQSH VTS
