SOX2_XENLA
ID SOX2_XENLA Reviewed; 311 AA.
AC O42569; O73690; Q6DFL8;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Transcription factor Sox-2;
DE Short=XSox2;
DE Short=XlSox-2;
DE AltName: Full=SRY (sex determining region Y)-box 2;
GN Name=sox2;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Dorsal lip;
RX PubMed=9598349;
RA De Robertis E.M., Kim S., Leyns L., Piccolo S., Bachiller D., Agius E.,
RA Belo J.A., Yamamoto A., Hainski-Brousseau A., Brizuela B., Wessely O.,
RA Lu B., Bouwmeester T.;
RT "Patterning by genes expressed in Spemann's organizer.";
RL Cold Spring Harb. Symp. Quant. Biol. 62:169-175(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RX PubMed=9435279; DOI=10.1242/dev.125.4.579;
RA Mizuseki K., Kishi M., Matsui M., Nakanishi S., Sasai Y.;
RT "Xenopus Zic-related-1 and Sox-2, two factors induced by chordin, have
RT distinct activities in the initiation of neural induction.";
RL Development 125:579-587(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Gastrula;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=10648237; DOI=10.1242/dev.127.4.791;
RA Kishi M., Mizuseki K., Sasai N., Yamazaki H., Shiota K., Nakanishi S.,
RA Sasai Y.;
RT "Requirement of Sox2-mediated signaling for differentiation of early
RT Xenopus neuroectoderm.";
RL Development 127:791-800(2000).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=17056008; DOI=10.1016/j.bbrc.2006.10.040;
RA Nitta K.R., Takahashi S., Haramoto Y., Fukuda M., Onuma Y., Asashima M.;
RT "Expression of Sox1 during Xenopus early embryogenesis.";
RL Biochem. Biophys. Res. Commun. 351:287-293(2006).
RN [6]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RA Ma L., Zhao S.-H., Kong Q.-H., Mao B.-Y.;
RT "Temporal and spatial expression patterns of Sox1 gene in Xenopus laevis
RT embryo.";
RL Dong Wu Xue Yan Jiu 28:403-408(2007).
CC -!- FUNCTION: Transcriptional activator (By similarity). Functions as a
CC switch in neuronal development, participating in the differentiation of
CC embryonic neuroectodermal cells into neural tissues by making the
CC ectodermal cells responsive to FGF-neuralizing signals. Downstream SRRT
CC target that mediates the promotion of neural stem cell self-renewal (By
CC similarity). {ECO:0000250, ECO:0000269|PubMed:10648237,
CC ECO:0000269|PubMed:9435279}.
CC -!- INTERACTION:
CC O42569; Q91813: otx2-a; NbExp=5; IntAct=EBI-15695825, EBI-15695802;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}.
CC -!- TISSUE SPECIFICITY: By mid-gastrulation (stage 11), strongly expressed
CC on the dorsal side of the embryo and by stage 13, expression marks the
CC future neural plate including the neural crest. During neurulation,
CC neural precursors showing expression converge to form the central
CC nervous system and eye. At stage 23 (early tailbud), expressed in the
CC primitive brain and optic vesicle, with expression high in the anterior
CC neural tube. In the tail bud brain, expressed in several patches along
CC the anterior-posterior axis in the telencephalon, midbrain-hindbrain
CC boundary and hindbrain. Also expressed in the branchial arches during
CC tailbud stages. In the tailbud eye, expressed in both the neural retina
CC and the lens. {ECO:0000269|PubMed:17056008, ECO:0000269|PubMed:9435279,
CC ECO:0000269|PubMed:9598349, ECO:0000269|Ref.6}.
CC -!- DEVELOPMENTAL STAGE: Expressed zygotically from gastrula stage (stage
CC 11). Expression remains constant at later stages.
CC {ECO:0000269|PubMed:17056008, ECO:0000269|PubMed:9435279,
CC ECO:0000269|Ref.6}.
CC -!- INDUCTION: By bmp-antagonism. By chordin. {ECO:0000269|PubMed:9435279}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:P41225}.
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DR EMBL; AF005476; AAB62821.1; -; mRNA.
DR EMBL; AF022928; AAC14215.1; -; mRNA.
DR EMBL; BC076717; AAH76717.1; -; mRNA.
DR RefSeq; NP_001081691.1; NM_001088222.1.
DR RefSeq; XP_018121173.1; XM_018265684.1.
DR AlphaFoldDB; O42569; -.
DR BMRB; O42569; -.
DR SMR; O42569; -.
DR DIP; DIP-29886N; -.
DR IntAct; O42569; 1.
DR MaxQB; O42569; -.
DR GeneID; 108718087; -.
DR GeneID; 398000; -.
DR KEGG; xla:398000; -.
DR CTD; 398000; -.
DR Xenbase; XB-GENE-865099; sox2.L.
DR OrthoDB; 1161594at2759; -.
DR Proteomes; UP000186698; Chromosome 5L.
DR Bgee; 108718087; Expressed in neurula embryo and 11 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR032643; SOX-2.
DR InterPro; IPR022097; SOX_fam.
DR PANTHER; PTHR10270:SF231; PTHR10270:SF231; 1.
DR Pfam; PF00505; HMG_box; 1.
DR Pfam; PF12336; SOXp; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Differentiation; DNA-binding;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..311
FT /note="Transcription factor Sox-2"
FT /id="PRO_0000048719"
FT DNA_BIND 37..105
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 266..274
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:P41225"
FT COMPBIAS 14..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 168..172
FT /note="MQEQL -> IEDDV (in Ref. 2; AAC14215)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="Q -> L (in Ref. 2; AAC14215)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="G -> R (in Ref. 2; AAC14215)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="A -> P (in Ref. 3; AAH76717)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 311 AA; 34106 MW; 4DED7A21560C7426 CRC64;
MYSMMETELK PPAPQQPSGG NSNSASNNQN KNSPDRVKRP MNAFMVWSRG QRRKMAQENP
KMHNSEISKR LGAEWKLLSE AEKRPFIDEA KRLRALHMKE HPDYKYRPRR KTKTLMKKDK
YTLPGGLLAP GANAMTSGVG GSLGAGVNQR MDTYAHMNGW TNGGYGMMQE QLGYPQHPGL
NAHNAPQMQP MHRYDVSALQ YNSMSSSQTY MNGSPTYSMS YSQQGAPGMS LGSMGSVVKS
ESSSSPPVVT SSSHSRAPCQ AGDLRDMISM YLPGAEVPES AAQSRLHMSQ HYQSASVAGT
GINGTLPLSH M
