ID   SOX3B_XENLA             Reviewed;         307 AA.
AC   Q5FWM3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Transcription factor Sox-3-B;
DE   AltName: Full=Transcription factor Sox-11;
DE            Short=xSox-11;
GN   Name=sox3-b; Synonyms=sox11;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000312|EMBL:AAH89283.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg {ECO:0000312|EMBL:AAH89283.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 51-104.
RC   TISSUE=Oocyte {ECO:0000269|PubMed:1614875};
RX   PubMed=1614875; DOI=10.1093/nar/20.11.2887;
RA   Denny P., Swift S., Brand N., Dabhade N., Barton P., Ashworth A.;
RT   "A conserved family of genes related to the testis determining gene, SRY.";
RL   Nucleic Acids Res. 20:2887-2887(1992).
CC   -!- FUNCTION: Transcription factor with sequence-specific DNA binding
CC       activity. Binds to the consensus sequence 5'-[AT][AT]CAA[AT]G-3',
CC       showing a preference for 5'-AACAAT-3' and 5'-AACAAAG-3'. Inhibits beta-
CC       catenin-mediated dorsal axis specification by binding to sites within
CC       the promoter of the beta-catenin-regulated gene nodal5. Acts maternally
CC       as a transcriptional repressor of nodal5 and nodal6 to restrict their
CC       expression to the vegetal hemisphere of early embryos and thus
CC       establish germ layer formation. Acts at multiple points to inhibit
CC       nodal signaling, repressing the expression of the other mesoderm-
CC       inducing nodal genes nodal, nodal2 and nodal4, and also acting
CC       downstream to induce expression of genes including trim33/ectodermin,
CC       ema and coco, whose products repress nodal signaling (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ctnnb1. {ECO:0000250|UniProtKB:P55863}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P55863,
CC       ECO:0000255|PROSITE-ProRule:PRU00267}. Cytoplasm
CC       {ECO:0000250|UniProtKB:P55863}. Note=Primarily cytoplasmic in early
CC       embryos. Nuclear localization becomes more pronounced as development
CC       proceeds (By similarity). {ECO:0000250|UniProtKB:P55863}.
CC   -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC       number of yeast and animal transcription factors.
CC       {ECO:0000250|UniProtKB:P41225}.
CC   -!- CAUTION: Was originally termed sox-11. {ECO:0000305|PubMed:1614875}.
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DR   EMBL; BC089283; AAH89283.1; -; mRNA.
DR   EMBL; X65654; CAA46605.1; -; mRNA.
DR   RefSeq; NP_001166875.1; NM_001173404.1.
DR   AlphaFoldDB; Q5FWM3; -.
DR   SMR; Q5FWM3; -.
DR   DNASU; 398329; -.
DR   GeneID; 398329; -.
DR   KEGG; xla:398329; -.
DR   CTD; 398329; -.
DR   OMA; AYPQHHG; -.
DR   OrthoDB; 1161594at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 398329; Expressed in blastula and 11 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0008013; F:beta-catenin binding; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0001704; P:formation of primary germ layer; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR029550; SOX-3.
DR   InterPro; IPR022097; SOX_fam.
DR   PANTHER; PTHR10270:SF111; PTHR10270:SF111; 1.
DR   Pfam; PF00505; HMG_box; 1.
DR   Pfam; PF12336; SOXp; 1.
DR   SMART; SM00398; HMG; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; DNA-binding; Gastrulation; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..307
FT                   /note="Transcription factor Sox-3-B"
FT                   /id="PRO_0000315865"
FT   DNA_BIND        40..108
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           257..268
FT                   /note="9aaTAD"
FT                   /evidence="ECO:0000250|UniProtKB:P41225"
FT   COMPBIAS        8..39
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   307 AA;  33825 MW;  F949C0A20CA87EC6 CRC64;
     MYSMLDTDMK SPVQQSNALS GGPGTPGGKG NTSTPDQDRV KRPMNAFMVW SRGQRRKMAQ
     ENPKMHNSEI SKRLGADWKL LSDSEKRPFI DEAKRLRAVH MKDYPDYKYR PRRKTKTLLK
     KDKYSLPGNL LAPGINPVSG GVGQRIDTYP HMNGWTNGAY SLMQEQLGYG QHPAMNSSQM
     QQIQHRYDMG GLQYSPMMSS AQTYMNAAAS TYSMSPAYNQ QSSTVMSLAS MGSVVKSEPS
     SPPPAITSHT QRACLGDLRD MISMYLPPGG DAGDHSSLQN SRLHSVHQHY QSAGGPGVNG
     TVPLTHI