SOX3_XENTR
ID SOX3_XENTR Reviewed; 307 AA.
AC Q68FA4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Transcription factor Sox-3;
GN Name=sox3 {ECO:0000312|EMBL:AAH79938.1};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000312|EMBL:AAH79938.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Tail bud {ECO:0000312|EMBL:AAH79938.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor with sequence-specific DNA binding
CC activity. Binds to the consensus sequence 5'-[AT][AT]CAA[AT]G-3',
CC showing a preference for 5'-AACAAT-3' and 5'-AACAAAG-3'. Inhibits beta-
CC catenin-mediated dorsal axis specification by binding to sites within
CC the promoter of the beta-catenin-regulated gene nodal5. Maternally
CC derived sox3 acts as a transcriptional repressor of nodal5 and nodal6
CC to restrict their expression to the vegetal hemisphere of early embryos
CC and thus establish germ layer formation. Acts at multiple points to
CC inhibit nodal signaling, repressing the expression of the other
CC mesoderm-inducing nodal genes nodal, nodal2 and nodal4, and also acting
CC downstream to induce expression of genes including trim33/ectodermin,
CC ema and coco, whose products repress nodal signaling (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with ctnnb1. {ECO:0000250|UniProtKB:P55863}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P55863,
CC ECO:0000255|PROSITE-ProRule:PRU00267}. Cytoplasm
CC {ECO:0000250|UniProtKB:P55863}. Note=Primarily cytoplasmic in early
CC embryos. Nuclear localization becomes more pronounced as development
CC proceeds (By similarity). {ECO:0000250|UniProtKB:P55863}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:P41225}.
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DR EMBL; BC079938; AAH79938.1; -; mRNA.
DR RefSeq; NP_001007502.1; NM_001007501.1.
DR AlphaFoldDB; Q68FA4; -.
DR SMR; Q68FA4; -.
DR PaxDb; Q68FA4; -.
DR DNASU; 493228; -.
DR Ensembl; ENSXETT00000016558; ENSXETP00000016558; ENSXETG00000007607.
DR GeneID; 493228; -.
DR KEGG; xtr:493228; -.
DR CTD; 6658; -.
DR Xenbase; XB-GENE-484815; sox3.
DR eggNOG; KOG0527; Eukaryota.
DR HOGENOM; CLU_021123_0_0_1; -.
DR InParanoid; Q68FA4; -.
DR OMA; ANAGGGX; -.
DR OrthoDB; 1161594at2759; -.
DR PhylomeDB; Q68FA4; -.
DR TreeFam; TF351735; -.
DR Reactome; R-XTR-3769402; Deactivation of the beta-catenin transactivating complex.
DR Proteomes; UP000008143; Chromosome 8.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000007607; Expressed in 2-cell stage embryo and 36 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0008013; F:beta-catenin binding; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0001704; P:formation of primary germ layer; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR029550; SOX-3.
DR InterPro; IPR022097; SOX_fam.
DR PANTHER; PTHR10270:SF111; PTHR10270:SF111; 1.
DR Pfam; PF00505; HMG_box; 1.
DR Pfam; PF12336; SOXp; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; DNA-binding; Gastrulation; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..307
FT /note="Transcription factor Sox-3"
FT /id="PRO_0000315866"
FT DNA_BIND 40..108
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 259..270
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:P41225"
FT COMPBIAS 8..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 307 AA; 33780 MW; C3DB985E4CC58174 CRC64;
MYSMLDTDLK SPVQQSNAPN GGPGTPGGKG NASIPDQERV KRPMNAFMVW SRGQRRKMAQ
ENPKMHNSEI SKRLGADWKL LSDSEKRPFI DEAKRLRAVH MKEYPDYKYR PRRKTKTLLK
KDKYSLPGNL LAPGVSPVAS SVGVGQRIDT YAHMNGWTNG AYSLMQDQLG YSQHPGMNSP
QMQQIQHRYD MGGLQYSPMM SSAQTYMNAA ASTYSMSPAY NQQSSTVMSL GSMGSVVKSE
PSSPPPAITS HTQRACLGDL RDMISMYLPP GGDASDPSSL QSSRLHSVHQ HYQSAAGPNG
TVPLTHI
