ID   SOXA_ARTSP              Reviewed;         967 AA.
AC   Q9AGP1;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Sarcosine oxidase subunit alpha;
DE            Short=Sarcosine oxidase subunit A;
DE            EC=1.5.3.1;
GN   Name=soxA;
OS   Arthrobacter sp.
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=1667;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, AND SUBUNIT.
RC   STRAIN=1IN;
RX   DOI=10.1007/BF00127888;
RA   Meskys R., Rudomanskis R., Leipuviene R.;
RT   "Cloning of genes encoding heterotetrameric sarcosine oxidase from
RT   Arthrobacter sp.";
RL   Biotechnol. Lett. 18:781-786(1996).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC       glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC       {ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC         Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:57433; EC=1.5.3.1;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|Ref.1};
CC   -!- SUBUNIT: Heterotetramer (SoxA, SoxB, SoxG and SoxD).
CC       {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000305}.
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DR   EMBL; AF329478; AAK16489.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AGP1; -.
DR   SMR; Q9AGP1; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   Gene3D; 3.10.20.440; -; 1.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR042204; 2Fe-2S-bd_N.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR006277; Sarcosine_oxidase_asu.
DR   InterPro; IPR041117; SoxA_A3.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF17806; SO_alpha_A3; 1.
DR   PIRSF; PIRSF037980; SoxA; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01372; soxA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN           1..967
FT                   /note="Sarcosine oxidase subunit alpha"
FT                   /id="PRO_0000428955"
SQ   SEQUENCE   967 AA;  102442 MW;  B4CD54F4967714DA CRC64;
     MSQNKSYRLP AEQSPAARID RGEALVLSVD GKQLDAFRGD TVASAMLANG QRSCGNSMYL
     DRPRGIFSAG VEEPNALVTV AARHEEDINE SMLAATTVPV TANLSATLLR GLGVLDPSTD
     PAYYDHVHVH TDVLVVGAGP AGLAAAREAS RSGARVLLLD ERAEAGGSLR DAAGEQIDGQ
     DAAAWIDATA AELASAAETT HLQRTTVLGS YDANYVIAAQ RRTVHLDAPS GAGVSRERIW
     HIRANQVVLA TGAHERPIVF QNNDRPGIML AGAVRSYLNR YGVRAGTRIV VATTNDSAYP
     LVADLAASGG VVAVVDARTT VSAAAAEAVG AGVRVITGSV VADTEANESG ELSAVVVAEL
     GEDRELGAPQ RFEADVLAVA GGFNPVVHLH SQRQGKLVWD TSIHAFVPDT AVANQHLAGA
     LTGLFDTASA LSTGAATGAA AATAAGFERI AQVPQALAVP AGEARPVWLV PSLNGDQAAN
     YTTHFVDLQR DQTVSDVLRA TGAGLESVEH IKRYTSISTA NDQGKTSGVA AIGVIAAVLG
     IENPAEIGTT TFRAPYTPVS FAALAGRTRG ALLDPARITP MHSWHLAHGA AFEDVGQWKR
     AWYFPQDGED MDAAVYRESK AVRDSVGMLD ATTLGKIEIR GKDAAEFLNR IYTNGYTKLK
     VGMARYGVMC KADGMVFDDG VTLRLAEDRF LMHTTTGGAA GVLDWLEEWL QTEWPELDVT
     CTSVTEQLAT VAVVGPRSRD VVAKLASGLD VSNEAFKFMS FRDVTLDSGI EARISRISFS
     GELAYEIAIP SWHGLRVWKD VFAAGQEFNI TPYGTETMHV LRAEKGFIIV GQDTDGTVTP
     QDAGMEWVVS KLKDFVGKRS FARADNLRED RKHLVSVLPV DTTLRLAEGA ALVADGAVET
     GGCTPMEGWV TSSYNSPALG RTFGLALIKN GRSRIGEVLK TPVNGQLVDV LVSDLVLFDP
     EGSRRDG