ID   SOXA_CORS1              Reviewed;         967 AA.
AC   Q46337;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Sarcosine oxidase subunit alpha;
DE            Short=Sarcosine oxidase subunit A;
DE            EC=1.5.3.1;
GN   Name=soxA;
OS   Corynebacterium sp. (strain P-1).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium; unclassified Corynebacterium.
OX   NCBI_TaxID=69006;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-30.
RX   PubMed=7543100; DOI=10.1074/jbc.270.31.18252;
RA   Chlumsky L.J., Zhang L., Jorns M.S.;
RT   "Sequence analysis of sarcosine oxidase and nearby genes reveals homologies
RT   with key enzymes of folate one-carbon metabolism.";
RL   J. Biol. Chem. 270:18252-18259(1995).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC       glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC         Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:57433; EC=1.5.3.1;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SUBUNIT: Heterotetramer (SoxA, SoxB, SoxG and SoxD).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U23955; AAC43461.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q46337; -.
DR   SMR; Q46337; -.
DR   KEGG; ag:AAC43461; -.
DR   BioCyc; MetaCyc:MON-8521; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   Gene3D; 3.10.20.440; -; 1.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR042204; 2Fe-2S-bd_N.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR006277; Sarcosine_oxidase_asu.
DR   InterPro; IPR041117; SoxA_A3.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF17806; SO_alpha_A3; 1.
DR   PIRSF; PIRSF037980; SoxA; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01372; soxA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:7543100"
FT   CHAIN           2..967
FT                   /note="Sarcosine oxidase subunit alpha"
FT                   /id="PRO_0000122627"
FT   REGION          132..160
FT                   /note="ADP binding"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   967 AA;  102767 MW;  C5F8A61F021134E9 CRC64;
     MSQNTSYRLP AEQSPAARID RGEALVLTVD GKQLEAFRGD TVASAMLANG QRACGNSMYL
     DRPRGIFSAG VEEPNALVTV EARHEQDINE SMLAATTVPV TANLSATLLR GLGVLDPSTD
     PAYYDHVHVH TDVLVVGAGP AGLAAAREAS RSGARVLLLD ERAEAGGSLR DAAGEQIDGQ
     DAAAWIDATV AELAAAEETT HLQRTTVLGS YDANYVVAVQ RRTVHLDGPS GAGVSRERIW
     HIRANQVVLA TGAHERPIVF ENNDRPGIML AGAVRSYLNC YGVRAGSQIV VATTNDSAYP
     LVADLAASGG VVAVIDARTT VSAAAAEAVG AGVRVITGSV VVDTEANESG ELSAVIVAEL
     GEDRELGEPQ RFEADVLAVA GGFNPVVHLH SQRQGKLVWD TSIHAFVPDA AVANQHLAGA
     LTGLFDTASA LSTGAAVGAA AATAAGFERI AQVPQALPVP AGETRPVWLV PSLNGDQATN
     YTTHFVDLQR DQTVSDVLRA TGAGLESVEH IKRYTSISTA NDQGKTSGVA AIGVIAAVLG
     IENPAEIGTT TFRAPYTPVA FAALAGRTRG ELLDPARITP MHSWHLAQGA KFEDVGQWKR
     AWYFPQDGED MDAAVYRECA AVRESVGMLD ATTLGKIEIR GADAAEFLNR IYTNGYTKLK
     VGMARYGVMC KADGMVFDDG VTLRLAEDRF LMHTTTGGAA GVLDWLEEWL QTEWPELDVT
     CTSVTEQLAT VAVVGPRSRD VVAKLVTGLD VSNDAFKFMS FQDVTLDSGI EARISRISFS
     GELAYEIAIP SWHGLRVWED VYAAGQEFNI TPYGTETMHV LRAEKGFIIV GQDTDGTVTP
     QDAGMEWVVS KLKDFVGKRS FSREDNLRED RKHLVSVLPV DTALRLAEGA ALVADGAVET
     EGCTPMEGWV TSSYNSPALG RTFGLALIKN GRSRIGEVLK TPVNGQLVDV LVSDLVLFDP
     EGSRRDG