ID   SOXB_ARTSP              Reviewed;         405 AA.
AC   Q9AGP3;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Sarcosine oxidase subunit beta;
DE            Short=Sarcosine oxidase subunit B;
DE            EC=1.5.3.1;
GN   Name=soxB;
OS   Arthrobacter sp.
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=1667;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, AND SUBUNIT.
RC   STRAIN=1IN;
RX   DOI=10.1007/BF00127888;
RA   Meskys R., Rudomanskis R., Leipuviene R.;
RT   "Cloning of genes encoding heterotetrameric sarcosine oxidase from
RT   Arthrobacter sp.";
RL   Biotechnol. Lett. 18:781-786(1996).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC       glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC       {ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC         Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:57433; EC=1.5.3.1;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|Ref.1};
CC       Note=Binds 1 FAD per subunit. {ECO:0000269|Ref.1};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|Ref.1};
CC       Note=Binds 1 FMN covalently. {ECO:0000269|Ref.1};
CC   -!- SUBUNIT: Heterotetramer (SoxA, SoxB, SoxG and SoxD).
CC       {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SoxB family. {ECO:0000305}.
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DR   EMBL; AF329478; AAK16487.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AGP3; -.
DR   SMR; Q9AGP3; -.
DR   PRIDE; Q9AGP3; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR001763; Rhodanese-like_dom.
DR   InterPro; IPR006278; SoxB.
DR   Pfam; PF01266; DAO; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01373; soxB; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; FAD; Flavoprotein; FMN; Oxidoreductase.
FT   CHAIN           1..405
FT                   /note="Sarcosine oxidase subunit beta"
FT                   /id="PRO_0000428957"
FT   MOD_RES         173
FT                   /note="Tele-8alpha-FMN histidine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   405 AA;  44041 MW;  523A7C820E3893DD CRC64;
     MADLLPEHPE FLWANPEPKK SYDVVIVGGG GHGLATAYYL AKNHGITNVA VLEKGWLAGG
     NMARNTTIIR SNYLWDESAG IYEKSLKLWE QLPEELDYDF LFSQRGVLNL AHTLGDVRES
     IRRVEANKFN GVDAEWLTPE QVKEVCPIIN IGDDIRYPVM GATYQPRAGI AKHDHVAWAF
     ARKANEMGVD IIQNCEVTGF LKDGEKVTGV KTTRGTIHAG KVALAGAGHS SVLAELAGFE
     LPIQSHPLQA LVSELFEPVH PTVVMSNHIH VYVSQAHKGE LVMGAGIDTY NGYGQRGAFH
     VIEEQMAAAV ELFPIFARAH VLRTWGGIVD TTMDALPIIS KTPIQNLYVN CGWGTGGFKG
     TPGAGFTLAH TIANDEAHAL NAPFSLERFE TGHLIDEHGA AAVAH