SOXB_CORS1
ID SOXB_CORS1 Reviewed; 405 AA.
AC P40875; Q46335;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Sarcosine oxidase subunit beta;
DE Short=Sarcosine oxidase subunit B;
DE EC=1.5.3.1;
GN Name=soxB;
OS Corynebacterium sp. (strain P-1).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium; unclassified Corynebacterium.
OX NCBI_TaxID=69006;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-36.
RX PubMed=7543100; DOI=10.1074/jbc.270.31.18252;
RA Chlumsky L.J., Zhang L., Jorns M.S.;
RT "Sequence analysis of sarcosine oxidase and nearby genes reveals homologies
RT with key enzymes of folate one-carbon metabolism.";
RL J. Biol. Chem. 270:18252-18259(1995).
RN [2]
RP PROTEIN SEQUENCE OF 161-187 AND 334-404.
RC STRAIN=U-96;
RX PubMed=1939012; DOI=10.1093/oxfordjournals.jbchem.a123479;
RA Suzuki H., Kawamura-Konishi Y.;
RT "Cysteine residues in the active site of Corynebacterium sarcosine
RT oxidase.";
RL J. Biochem. 109:909-917(1991).
RN [3]
RP PARTIAL PROTEIN SEQUENCE.
RC STRAIN=U-96;
RX PubMed=3202887;
RA Suzuki H., Kawamura-Konishi Y.;
RT "Presence of AMP binding sequence in subunit B of Corynebacterium sarcosine
RT oxidase.";
RL Biochem. Int. 17:577-583(1988).
RN [4]
RP CHARACTERIZATION, AND MUTAGENESIS OF HIS-175.
RX PubMed=7692961; DOI=10.1021/bi00092a024;
RA Chlumsky L.J., Zhang L., Ramsey A.J., Jorns M.S.;
RT "Preparation and properties of recombinant corynebacterial sarcosine
RT oxidase: evidence for posttranslational modification during turnover with
RT sarcosine.";
RL Biochemistry 32:11132-11142(1993).
RN [5]
RP FMN-BINDING.
RX PubMed=8611516; DOI=10.1021/bi952995h;
RA Willie A., Edmondson D.E., Jorns M.S.;
RT "Sarcosine oxidase contains a novel covalently bound FMN.";
RL Biochemistry 35:5292-5299(1996).
RN [6]
RP PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=9485355; DOI=10.1021/bi972705s;
RA Chlumsky L.J., Sturgess A.W., Nieves E., Jorns M.S.;
RT "Identification of the covalent flavin attachment site in sarcosine
RT oxidase.";
RL Biochemistry 37:2089-2095(1998).
RN [7]
RP PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=11902668; DOI=10.1023/a:1014135216860;
RA Mukouyama E.B., Ohsawa H., Suzuki H.;
RT "Cofactors in sarcosine oxidase from Corynebacterium sp. U-96.";
RL J. Protein Chem. 21:59-64(2002).
CC -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; EC=1.5.3.1;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Note=Binds 1 FAD per subunit.;
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Note=Binds 1 FMN covalently.;
CC -!- SUBUNIT: Heterotetramer (SoxA, SoxB, SoxG and SoxD).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the SoxB family. {ECO:0000305}.
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DR EMBL; U23955; AAC43459.1; -; Genomic_DNA.
DR PIR; PX0049; PX0049.
DR AlphaFoldDB; P40875; -.
DR SMR; P40875; -.
DR KEGG; ag:AAC43459; -.
DR BioCyc; MetaCyc:MON-8522; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR006278; SoxB.
DR Pfam; PF01266; DAO; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR01373; soxB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; FMN;
KW Oxidoreductase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7543100"
FT CHAIN 2..405
FT /note="Sarcosine oxidase subunit beta"
FT /id="PRO_0000072044"
FT REGION 23..53
FT /note="ADP binding"
FT /evidence="ECO:0000255"
FT MOD_RES 173
FT /note="Tele-8alpha-FMN histidine"
FT VARIANT 167
FT /note="R -> Y (in strain: U-96)"
FT VARIANT 172..173
FT /note="KH -> CK (in strain: U-96)"
FT VARIANT 178
FT /note="W -> T (in strain: U-96)"
FT VARIANT 182
FT /note="R -> N (in strain: U-96)"
FT VARIANT 359
FT /note="K -> FC (in strain: U-96)"
FT VARIANT 366
FT /note="F -> Y (in strain: U-96)"
FT VARIANT 370
FT /note="H -> E (in strain: U-96)"
FT VARIANT 374
FT /note="N -> A (in strain: U-96)"
FT VARIANT 377..379
FT /note="AHA -> PKK (in strain: U-96)"
FT VARIANT 385
FT /note="S -> A (in strain: U-96)"
FT MUTAGEN 175
FT /note="H->A: No effect on FMN binding."
FT /evidence="ECO:0000269|PubMed:7692961"
SQ SEQUENCE 405 AA; 43987 MW; 401141810BA60CDE CRC64;
MADLLPEHPE FLWANPEPKK SYDVVIVGGG GHGLATAYYL AKNHGITNVA VLEKGWLAGG
NMARNTTIIR SNYLWDESAG IYEKSLKLWE QLPEELDYDF LFSQRGVLNL AHTLGDVRES
VRRVEANKFN GVDAEWLTPE QVKEVCPIIN IGDDIRYPVM GATYQPRAGI AKHDHVAWAF
ARKANEMGVD IIQNCEVTGF LKDGEKVTGV KTTRGTIHAG KVALAGAGHS SVLAELAGFE
LPIQSHPLQA LVSELFEPVH PTVVMSNHIH VYVSQAHKGE LVMGAGIDSY NGYGQRGAFH
VIEEQMAAAV ELFPIFARAH VLRTWGGIVD TTMDASPIIS KTPIQNLYVN CGWGTGGFKG
TPGAGFTLAH TIANDEAHAL NAPFSLERFE TGHLIDEHGA AAVAH
