ID   SOXB_RHIME              Reviewed;         416 AA.
AC   O87388;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Sarcosine oxidase subunit beta;
DE            Short=Sarcosine oxidase subunit B;
DE            EC=1.5.3.1;
GN   Name=soxB; OrderedLocusNames=R00085; ORFNames=SMc02608;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1021;
RA   Powers E.L., Vuyyuru V., Kahn M.L.;
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481430; DOI=10.1073/pnas.161294398;
RA   Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA   Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA   Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA   Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA   Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT   "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT   meliloti strain 1021.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC       glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC         Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC         ChEBI:CHEBI:57433; EC=1.5.3.1;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC       Note=Binds 1 FMN covalently. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SoxB family. {ECO:0000305}.
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DR   EMBL; AF055582; AAC62218.1; -; Genomic_DNA.
DR   EMBL; AL591688; CAC41472.1; -; Genomic_DNA.
DR   RefSeq; NP_384191.1; NC_003047.1.
DR   RefSeq; WP_003536326.1; NC_003047.1.
DR   AlphaFoldDB; O87388; -.
DR   SMR; O87388; -.
DR   STRING; 266834.SMc02608; -.
DR   EnsemblBacteria; CAC41472; CAC41472; SMc02608.
DR   GeneID; 61601566; -.
DR   KEGG; sme:SMc02608; -.
DR   PATRIC; fig|266834.11.peg.1442; -.
DR   eggNOG; COG0665; Bacteria.
DR   HOGENOM; CLU_007884_4_1_5; -.
DR   OMA; SSALHCY; -.
DR   Proteomes; UP000001976; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR001763; Rhodanese-like_dom.
DR   InterPro; IPR006278; SoxB.
DR   Pfam; PF01266; DAO; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01373; soxB; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; FMN; Oxidoreductase; Reference proteome.
FT   CHAIN           1..416
FT                   /note="Sarcosine oxidase subunit beta"
FT                   /id="PRO_0000072045"
FT   BINDING         33..63
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   416 AA;  45374 MW;  86FA18AAA0E9E324 CRC64;
     MRYSALSILL NGLRGNRNWA PAWRQPDPKP HYDVIIVGGG GHGLATAYYL AKEFGITNVA
     VLEKNYIGSG NVGRNTTIIR SNYLLPGNNP FYELSMKLWE GLEQDFNFNA MVSQRGVLNL
     FHSDAQRDAY TRRGNAMRLH GVDAELLYRA AVRKMLPFLD FDNARFPIQG GLLQRRGGTV
     RHDAVAWGYA RGADSRGVDI IQNCEVTGIR RENGRVIGVE TSRGFIGCAK LALAAAGNSS
     QVAEMAGLRL PIESHVLQAF VSEGLKPFID GVVTFGAGHF YVSQSDKGGL VFGGDIDGYN
     SYAQRGNLAT VEHVAEAGKA MIPALSRVRV LRSWGGIMDM SMDGSPIIDR TPIDNLYLNA
     GWCYGGFKAT PASGFCFAHL LARGAPQKTA AAFRLDRFER GFLIDEKGQG AQPNLH