SOXB_RHOCB
ID SOXB_RHOCB Reviewed; 414 AA.
AC Q52671; D5ALI5;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Sarcosine oxidase subunit beta;
DE Short=Sarcosine oxidase subunit B;
DE EC=1.5.3.1;
GN Name=soxB; OrderedLocusNames=RCAP_rcc02316;
OS Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=272942;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20418398; DOI=10.1128/jb.00366-10;
RA Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA Haselkorn R.;
RT "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT Rhodobacter capsulatus SB 1003.";
RL J. Bacteriol. 192:3545-3546(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-208.
RC STRAIN=MT1131;
RA Lang S.E., Jenney F.E. Jr., Daldal F.;
RT "Rhodobacter capsulatus cycH: a bipartite gene that affects differentially
RT the biogenesis of various c-type cytochromes.";
RL Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield
CC glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O2 + sarcosine = formaldehyde + glycine + H2O2;
CC Xref=Rhea:RHEA:13313, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16842, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:57433; EC=1.5.3.1;
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC Note=Binds 1 FMN covalently. {ECO:0000250};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SoxB family. {ECO:0000305}.
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DR EMBL; CP001312; ADE86046.1; -; Genomic_DNA.
DR EMBL; U46071; AAB01814.1; -; Genomic_DNA.
DR RefSeq; WP_013068025.1; NC_014034.1.
DR AlphaFoldDB; Q52671; -.
DR SMR; Q52671; -.
DR STRING; 272942.RCAP_rcc02316; -.
DR EnsemblBacteria; ADE86046; ADE86046; RCAP_rcc02316.
DR GeneID; 31491151; -.
DR KEGG; rcp:RCAP_rcc02316; -.
DR eggNOG; COG0665; Bacteria.
DR HOGENOM; CLU_007884_4_1_5; -.
DR OMA; PIKPCMD; -.
DR OrthoDB; 912110at2; -.
DR Proteomes; UP000002361; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR006278; SoxB.
DR Pfam; PF01266; DAO; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR01373; soxB; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; FMN; Oxidoreductase; Reference proteome.
FT CHAIN 1..414
FT /note="Sarcosine oxidase subunit beta"
FT /id="PRO_0000072046"
FT BINDING 34..64
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
SQ SEQUENCE 414 AA; 45018 MW; D7A5C411F9ABB411 CRC64;
MRRYSIFAVA REALRYHSGW EKAWASPEPK KRYDVIVVGA GGHGLATAYY LGKVHGIKNV
AIIEKGWLGG GNTGRNTTII RSNYLQDPSA AIYEKARSLY EGLSQDLNYN VMFSPRGLLM
LAQSEHEMRG YKRTVYANNL QNVETRWIEP KEVKKLVPIL NIAGPRYPVL GALLQEKGGT
ARHDAVAWGY ARACSDMGMD LLQNTEVTGV RTEGGRVVGV DTSRGSIDCG KLAIVVAGHS
SVLAGMAGFR LPIESMALQA WVSEPIKPTI DVVVMANLVH GYMSQSDKGE LVIGGGTDGF
NNYSQRGSWH HVEETTRALI ETFPVISRLK MLRQWGGIVD MTGDRSPILS KTPVDGIFVN
CGWGTGGFKA IPGSGFAMAE LVAKGYSPLA AEFGLHRFKE GQFIDESVAA GVAH
