SP0J_BACSU
ID SP0J_BACSU Reviewed; 282 AA.
AC P26497;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Stage 0 sporulation protein J;
GN Name=spo0J; OrderedLocusNames=BSU40960;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / CRK2000;
RX PubMed=1552862; DOI=10.1111/j.1365-2958.1992.tb01510.x;
RA Ogasawara N., Yoshikawa H.;
RT "Genes and their organization in the replication origin region of the
RT bacterial chromosome.";
RL Mol. Microbiol. 6:629-634(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 104-282.
RC STRAIN=168;
RX PubMed=1900505; DOI=10.1128/jb.173.6.1911-1919.1991;
RA Mysliwiec T.H., Errington J., Vaidya A.B., Bramucci M.G.;
RT "The Bacillus subtilis spo0J gene: evidence for involvement in catabolite
RT repression of sporulation.";
RL J. Bacteriol. 173:1911-1919(1991).
RN [5]
RP FUNCTION.
RC STRAIN=168 / JH642;
RX PubMed=8071208; DOI=10.1128/jb.176.17.5320-5329.1994;
RA Ireton K., Gunther N.W. IV, Grossman A.D.;
RT "spo0J is required for normal chromosome segregation as well as the
RT initiation of sporulation in Bacillus subtilis.";
RL J. Bacteriol. 176:5320-5329(1994).
CC -!- FUNCTION: Required for the initiation of sporulation and for normal
CC chromosome segregation. Antagonizes sporulation inhibition by Soj. It
CC probably interacts with a specific DNA site and other proteins involved
CC in partitioning and cell division, and antagonizes Soj in response to
CC cell cycle events related to chromosome partitioning.
CC {ECO:0000269|PubMed:8071208}.
CC -!- SIMILARITY: Belongs to the ParB family. {ECO:0000305}.
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DR EMBL; D26185; BAA05226.1; -; Genomic_DNA.
DR EMBL; X62539; CAA44409.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB16133.1; -; Genomic_DNA.
DR EMBL; M59938; AAA62694.1; -; Genomic_DNA.
DR PIR; I40445; A38536.
DR RefSeq; NP_391976.1; NC_000964.3.
DR RefSeq; WP_003226832.1; NZ_JNCM01000034.1.
DR PDB; 5NOC; NMR; -; A/B=217-282.
DR PDB; 6SDK; X-ray; 1.81 A; A/B/C/D=21-218.
DR PDBsum; 5NOC; -.
DR PDBsum; 6SDK; -.
DR AlphaFoldDB; P26497; -.
DR BMRB; P26497; -.
DR SMR; P26497; -.
DR IntAct; P26497; 3.
DR STRING; 224308.BSU40960; -.
DR jPOST; P26497; -.
DR PaxDb; P26497; -.
DR PRIDE; P26497; -.
DR EnsemblBacteria; CAB16133; CAB16133; BSU_40960.
DR GeneID; 937907; -.
DR KEGG; bsu:BSU40960; -.
DR PATRIC; fig|224308.179.peg.4438; -.
DR eggNOG; COG1475; Bacteria.
DR InParanoid; P26497; -.
DR OMA; IALSYQR; -.
DR PhylomeDB; P26497; -.
DR BioCyc; BSUB:BSU40960-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0043590; C:bacterial nucleoid; IDA:CACAO.
DR GO; GO:0005694; C:chromosome; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IMP:CACAO.
DR GO; GO:0045881; P:positive regulation of sporulation resulting in formation of a cellular spore; IGI:CACAO.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR041468; HTH_ParB/Spo0J.
DR InterPro; IPR004437; ParB/RepB/Spo0J.
DR InterPro; IPR003115; ParB/Sulfiredoxin_dom.
DR InterPro; IPR036086; ParB/Sulfiredoxin_sf.
DR Pfam; PF17762; HTH_ParB; 1.
DR Pfam; PF02195; ParBc; 1.
DR SMART; SM00470; ParB; 1.
DR SUPFAM; SSF110849; SSF110849; 1.
DR TIGRFAMs; TIGR00180; parB_part; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromosome partition; DNA-binding; Reference proteome;
KW Sporulation.
FT CHAIN 1..282
FT /note="Stage 0 sporulation protein J"
FT /id="PRO_0000178673"
FT DNA_BIND 139..158
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT CONFLICT 152
FT /note="I -> N (in Ref. 4; AAA62694)"
FT /evidence="ECO:0000305"
FT CONFLICT 172..173
FT /note="GT -> A (in Ref. 4; AAA62694)"
FT /evidence="ECO:0000305"
FT STRAND 21..27
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 28..30
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 44..57
FT /evidence="ECO:0007829|PDB:6SDK"
FT STRAND 63..67
FT /evidence="ECO:0007829|PDB:6SDK"
FT STRAND 69..76
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 78..86
FT /evidence="ECO:0007829|PDB:6SDK"
FT STRAND 90..96
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 101..113
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 120..134
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 138..145
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 149..158
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 163..170
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 176..183
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 188..190
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 191..201
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 205..216
FT /evidence="ECO:0007829|PDB:6SDK"
FT HELIX 232..245
FT /evidence="ECO:0007829|PDB:5NOC"
FT STRAND 249..252
FT /evidence="ECO:0007829|PDB:5NOC"
FT STRAND 258..266
FT /evidence="ECO:0007829|PDB:5NOC"
FT HELIX 267..278
FT /evidence="ECO:0007829|PDB:5NOC"
FT TURN 279..281
FT /evidence="ECO:0007829|PDB:5NOC"
SQ SEQUENCE 282 AA; 32210 MW; 49FB53511815F4E9 CRC64;
MAKGLGKGIN ALFNQVDLSE ETVEEIKIAD LRPNPYQPRK HFDDEALAEL KESVLQHGIL
QPLIVRKSLK GYDIVAGERR FRAAKLAGLD TVPAIVRELS EALMREIALL ENLQREDLSP
LEEAQAYDSL LKHLDLTQEQ LAKRLGKSRP HIANHLRLLT LPENIQQLIA EGTLSMGHGR
TLLGLKNKNK LEPLVQKVIA EQLNVRQLEQ LIQQLNQNVP RETKKKEPVK DAVLKERESY
LQNYFGTTVN IKRQKKKGKI EIEFFSNEDL DRILELLSER ES
