SP13A_ARATH
ID SP13A_ARATH Reviewed; 359 AA.
AC B9DI20; Q8LFW6; Q9LUF4;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Squamosa promoter-binding-like protein 13A;
GN Name=SPL13A; Synonyms=SPL13; OrderedLocusNames=At5g50570;
GN ORFNames=MBA10.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION.
RX PubMed=12202040; DOI=10.1016/s0092-8674(02)00863-2;
RA Rhoades M.W., Reinhart B.J., Lim L.P., Burge C.B., Bartel B., Bartel D.P.;
RT "Prediction of plant microRNA targets.";
RL Cell 110:513-520(2002).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=14573523; DOI=10.1242/dev.00842;
RA Schmid M., Uhlenhaut N.H., Godard F., Demar M., Bressan R., Weigel D.,
RA Lohmann J.U.;
RT "Dissection of floral induction pathways using global expression
RT analysis.";
RL Development 130:6001-6012(2003).
CC -!- FUNCTION: Trans-acting factor that binds specifically to the consensus
CC nucleotide sequence 5'-TNCGTACAA-3'. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000250};
CC -!- INTERACTION:
CC B9DI20; Q9LQT8: GAI; NbExp=3; IntAct=EBI-15206662, EBI-963606;
CC B9DI20; Q8GXW1: RGL2; NbExp=3; IntAct=EBI-15206662, EBI-963665;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Weak increase of expression during floral
CC induction. {ECO:0000269|PubMed:14573523}.
CC -!- INDUCTION: Negatively regulated by microRNAs miR156 and miR157.
CC {ECO:0000305|PubMed:12202040}.
CC -!- DOMAIN: The SBP-type zinc finger is required for the binding to DNA.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM61173.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB025619; BAB09142.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95962.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95963.1; -; Genomic_DNA.
DR EMBL; AK117475; BAC42139.1; -; mRNA.
DR EMBL; AK317730; BAH20387.1; -; mRNA.
DR EMBL; AY084609; AAM61173.1; ALT_INIT; mRNA.
DR RefSeq; NP_001332253.1; NM_001344905.1.
DR RefSeq; NP_568731.1; NM_124435.3.
DR RefSeq; NP_568740.1; NM_124445.4.
DR RefSeq; NP_851161.1; NM_180830.4.
DR AlphaFoldDB; B9DI20; -.
DR SMR; B9DI20; -.
DR BioGRID; 20372; 1.
DR BioGRID; 20384; 18.
DR IntAct; B9DI20; 19.
DR STRING; 3702.AT5G50570.1; -.
DR PaxDb; B9DI20; -.
DR PRIDE; B9DI20; -.
DR EnsemblPlants; AT5G50570.1; AT5G50570.1; AT5G50570.
DR EnsemblPlants; AT5G50570.2; AT5G50570.2; AT5G50570.
DR EnsemblPlants; AT5G50670.1; AT5G50670.1; AT5G50670.
DR EnsemblPlants; AT5G50670.2; AT5G50670.2; AT5G50670.
DR GeneID; 835126; -.
DR GeneID; 835138; -.
DR Gramene; AT5G50570.1; AT5G50570.1; AT5G50570.
DR Gramene; AT5G50570.2; AT5G50570.2; AT5G50570.
DR Gramene; AT5G50670.1; AT5G50670.1; AT5G50670.
DR Gramene; AT5G50670.2; AT5G50670.2; AT5G50670.
DR KEGG; ath:AT5G50570; -.
DR KEGG; ath:AT5G50670; -.
DR Araport; AT5G50570; -.
DR TAIR; locus:2159717; AT5G50570.
DR eggNOG; ENOG502QRGA; Eukaryota.
DR HOGENOM; CLU_042475_0_0_1; -.
DR InParanoid; B9DI20; -.
DR OMA; KTIWPES; -.
DR OrthoDB; 916847at2759; -.
DR PhylomeDB; B9DI20; -.
DR PRO; PR:B9DI20; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; B9DI20; differential.
DR Genevisible; B9DI20; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048653; P:anther development; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR Gene3D; 4.10.1100.10; -; 1.
DR InterPro; IPR004333; SBP_dom.
DR InterPro; IPR036893; SBP_sf.
DR InterPro; IPR044817; SPL.
DR PANTHER; PTHR31251; PTHR31251; 1.
DR Pfam; PF03110; SBP; 1.
DR SUPFAM; SSF103612; SSF103612; 1.
DR PROSITE; PS51141; ZF_SBP; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..359
FT /note="Squamosa promoter-binding-like protein 13A"
FT /id="PRO_0000132734"
FT ZN_FING 98..175
FT /note="SBP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT REGION 75..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 158..174
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 77..94
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 101
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 106
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 123
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 126
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 142
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 145
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 149
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT BINDING 161
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00470"
FT CONFLICT 334
FT /note="S -> C (in Ref. 5; AAM61173)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 359 AA; 39108 MW; D80B8B9FD03D349F CRC64;
MDWNFKLSSG YLSGFDQEPD LSPMDGSISF GGSSQSKADF SFDLKLGRNI GNSSSVFGDT
EQVISLSKWK DSALAKPEGS RSSSSKRTRG NGVGTNQMPI CLVDGCDSDF SNCREYHKRH
KVCDVHSKTP VVTINGHKQR FCQQCSRFHA LEEFDEGKRS CRKRLDGHNR RRRKPQPEHI
GRPANFFTGF QGSKLLEFSG GSHVFPTTSV LNPSWGNSLV SVAVAANGSS YGQSQSYVVG
SSPAKTGIMF PISSSPNSTR SIAKQFPFLQ EEESSRTASL CERMTSCIHD SDCALSLLSS
SSSSVPHLLQ PPLSLSQEAV ETVFYGSGLF ENASAVSDGS VISGNEAVRL PQTFPFHWE
