SP3AF_BACSU
ID SP3AF_BACSU Reviewed; 206 AA.
AC P49783;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Stage III sporulation protein AF;
GN Name=spoIIIAF; OrderedLocusNames=BSU24380;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RA Guerout-Fleury A.M., Gonzy-Treboul G., Stragier P.;
RL Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; U35252; AAA76725.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12565.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14369.1; -; Genomic_DNA.
DR PIR; A69712; A69712.
DR RefSeq; NP_390318.1; NC_000964.3.
DR RefSeq; WP_003230233.1; NZ_JNCM01000036.1.
DR PDB; 6DCS; X-ray; 2.70 A; A/B=85-206.
DR PDBsum; 6DCS; -.
DR AlphaFoldDB; P49783; -.
DR SMR; P49783; -.
DR STRING; 224308.BSU24380; -.
DR TCDB; 9.B.70.1.1; the multicomponent putative spoiiiae exporter (spoiiia-e) family.
DR PaxDb; P49783; -.
DR PRIDE; P49783; -.
DR EnsemblBacteria; CAB14369; CAB14369; BSU_24380.
DR GeneID; 938576; -.
DR KEGG; bsu:BSU24380; -.
DR PATRIC; fig|224308.179.peg.2656; -.
DR eggNOG; ENOG503322G; Bacteria.
DR OMA; IWEIGSE; -.
DR BioCyc; BSUB:BSU24380-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR014245; Spore_III_AF.
DR Pfam; PF09581; Spore_III_AF; 1.
DR TIGRFAMs; TIGR02896; spore_III_AF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Reference proteome; Sporulation;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..206
FT /note="Stage III sporulation protein AF"
FT /id="PRO_0000072070"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:6DCS"
FT HELIX 91..110
FT /evidence="ECO:0007829|PDB:6DCS"
FT STRAND 112..115
FT /evidence="ECO:0007829|PDB:6DCS"
FT STRAND 117..125
FT /evidence="ECO:0007829|PDB:6DCS"
FT HELIX 132..134
FT /evidence="ECO:0007829|PDB:6DCS"
FT STRAND 135..143
FT /evidence="ECO:0007829|PDB:6DCS"
FT HELIX 161..186
FT /evidence="ECO:0007829|PDB:6DCS"
FT HELIX 190..192
FT /evidence="ECO:0007829|PDB:6DCS"
FT STRAND 193..196
FT /evidence="ECO:0007829|PDB:6DCS"
FT STRAND 198..201
FT /evidence="ECO:0007829|PDB:6DCS"
SQ SEQUENCE 206 AA; 23291 MW; BD47F355B9F3D35D CRC64;
MSFLTEWLTT IVLFILFAIV IDMLLPSSSM QKYAKMVVSL LLIVVMLTPI FKLFKTDPEV
IFEYLTKNGQ SESADIKNQI NSKKIEIQAS QRAYILEEMA VQLKKKAEER FSHDEYKVGR
IKLTAGEKVD SEEDIKTISV YMAPSSEKTV QTVAPVHIDT DHAYVTKEAA EQKEAKQIQT
QLADIWEIGS EKITVHMEGG ESVGNE
