ID   SP4_BOMPE               Reviewed;         243 AA.
AC   Q7M4I3;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Venom protease;
DE            EC=3.4.21.-;
DE   AltName: Allergen=Bom p 4;
OS   Bombus pensylvanicus (American bumblebee) (Apis pensylvanica).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Bombus; Fervidobombus.
OX   NCBI_TaxID=28643;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Venom;
RA   Hoffman D.R.;
RL   Submitted (MAY-1995) to the PIR data bank.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC   -!- ALLERGEN: Causes an allergic reaction in human. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00274}.
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DR   PIR; A56338; A56338.
DR   AlphaFoldDB; Q7M4I3; -.
DR   SMR; Q7M4I3; -.
DR   Allergome; 154; Bom p 4.
DR   Allergome; 3158; Bom p 4.0101.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Hydrolase; Protease;
KW   Secreted; Serine protease.
FT   CHAIN           1..243
FT                   /note="Venom protease"
FT                   /id="PRO_0000401926"
FT   DOMAIN          1..243
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        49
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        97
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        193
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   DISULFID        34..50
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        165..178
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        189..217
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ   SEQUENCE   243 AA;  27251 MW;  4A4282AA17DC44EB CRC64;
     VVGGKPAKLG AWPWMVALGF HNYRQPKKSP EWKCGGSLRI SRHVLTAAHC AIHRSLYVVR
     IADLNLKRDD DGAHPIQMGI ESKLIHPDYV YSEHHDDIAI LKLEKDVSFS EYIRPICLPI
     EESLRNNNFI GYNPFVAGWG RLRYKGPLSD ALMEVQVPVV RNKVCKRAYS DVSDTVICAG
     YPKGRKDSCQ GDSGGPLMIP QESTYYEIGV VSYGHECALP KYPGVYTRVT SYLDSFILPA
     LKK