SP4_MOUSE
ID SP4_MOUSE Reviewed; 782 AA.
AC Q62445; E9QNI3;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 25-MAY-2022, entry version 152.
DE RecName: Full=Transcription factor Sp4;
GN Name=Sp4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8660867; DOI=10.1006/dbio.1996.0134;
RA Supp D.M., Witte D.P., Branford W.W., Smith E.P., Potter S.S.;
RT "Sp4, a member of the Sp1-family of zinc finger transcription factors, is
RT required for normal murine growth, viability, and male fertility.";
RL Dev. Biol. 176:284-299(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Binds to GT and GC boxes promoters elements. Probable
CC transcriptional activator. Required for normal male reproductive
CC behavior.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in many tissues.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in embryos in the developing
CC central nervous system (CNS). Expression is seen as early as day 9 of
CC development, where transcripts are abundant in the posterior neuropore.
CC Expression in later embryos is detected throughout the CNS as well as
CC in other structures.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors. In SP4, the motif is
CC inactive. {ECO:0000250|UniProtKB:Q02446}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; U62522; AAC52653.1; -; mRNA.
DR EMBL; AC163032; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC163034; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS36579.1; -.
DR AlphaFoldDB; Q62445; -.
DR SMR; Q62445; -.
DR STRING; 10090.ENSMUSP00000026367; -.
DR iPTMnet; Q62445; -.
DR PhosphoSitePlus; Q62445; -.
DR jPOST; Q62445; -.
DR MaxQB; Q62445; -.
DR PaxDb; Q62445; -.
DR PeptideAtlas; Q62445; -.
DR PRIDE; Q62445; -.
DR ProteomicsDB; 258713; -.
DR MGI; MGI:107595; Sp4.
DR eggNOG; KOG1721; Eukaryota.
DR InParanoid; Q62445; -.
DR ChiTaRS; Sp4; mouse.
DR PRO; PR:Q62445; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q62445; protein.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; ISS:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0008016; P:regulation of heart contraction; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:MGI.
DR InterPro; IPR030453; SP4.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR23235:SF17; PTHR23235:SF17; 2.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..782
FT /note="Transcription factor Sp4"
FT /id="PRO_0000047145"
FT ZN_FING 645..669
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 675..699
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 705..727
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 107..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 275..385
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 465..473
FT /note="9aaTAD; inactive"
FT /evidence="ECO:0000250|UniProtKB:Q02446"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..45
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q02446"
FT CONFLICT 42
FT /note="H -> Q (in Ref. 1; AAC52653)"
FT /evidence="ECO:0000305"
FT CONFLICT 183
FT /note="S -> R (in Ref. 1; AAC52653)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 782 AA; 81906 MW; CAFFF89C759EA9BC CRC64;
MSDQKKEEEE EAAAAMATEG GKTSEPENNN KKPKTSGSQD SHPSPLALLA ATCSKIGTPG
ENQATGQQQI IIDPSQGLVQ LQNQPQQLEL VTTQLAGNAW QLVASTPPAS KENNVSQPAS
SSSSSSSSNN GSSSPTKTKS GNPSTPNQFQ VIQVQNPSGS VQYQVIPQLQ TVEGQQIQIN
PTSSSSLQDL QGQIQLISAG NNQAILTAAN RTASGNILAQ NLANQTVPVQ IRPGVSIPLQ
LQTLPGTQAQ VVTTLPINIG GVTLALPVIN NVTAGGGTGQ VGQPTTTTDS GTSNGNQLVS
TPTTSTAPAS TMPESPSSST TCTTTASTTL TSSDTLVSSA DTGQYASTSA SSSERTIEEP
QTPAATESEA QSSSQLQSNG IQNAQDQSNS LQQVQIVGQP ILQQIQIQQP QQQIIQAIPP
QSFQLQSGQT IQTIQQQPLQ NVQLQAVNPT QVLIRAPTLT PSGQISWQTV QVQNIQSLSN
LQVQNAGLSQ QLTITPVSSS GGTTLAQIAP VAVAGAPITL NTAQLASVPN LQTVSVANLG
AAGVQVQGVP VTITSVAGQQ QGQDGVKVQQ ATIAPVTVAV GGIANATIGA VSPDQLTQVH
LQQGQQTSDA EVQPGKRLRR VACSCPNCRE GEGRGSSEPG KKKQHVCHIE GCGKVYGKTS
HLRAHLRWHT GERPFICNWM FCGKRFTRSD ELQRHRRTHT GEKRFECPEC SKRFMRSDHL
SKHVKTHQNK KGGGTALAIV TSGELDSSVT EVLGSPRIVT VAAISQDSNP ATPNVSTNME
EF
