ID   SP4_PRIM3               Reviewed;         395 AA.
AC   P41028; D5DMN2;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 2.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Putative stage IV sporulation protein;
GN   Name=spoIV; OrderedLocusNames=BMD_4540;
OS   Priestia megaterium (strain DSM 319 / IMG 1521) (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=592022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 319 / IMG 1521;
RX   PubMed=21705586; DOI=10.1128/jb.00449-11;
RA   Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K.,
RA   Koenig S.S., Creasy H.H., Rosovitz M.J., Riley D.R., Daugherty S.,
RA   Martin M., Elbourne L.D., Paulsen I., Biedendieck R., Braun C.,
RA   Grayburn S., Dhingra S., Lukyanchuk V., Ball B., Ul-Qamar R., Seibel J.,
RA   Bremer E., Jahn D., Ravel J., Vary P.S.;
RT   "Genome sequences of the biotechnologically important Bacillus megaterium
RT   strains QM B1551 and DSM319.";
RL   J. Bacteriol. 193:4199-4213(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-280.
RX   PubMed=7764969; DOI=10.1007/bf00221230;
RA   Meinhardt F., Busskamp M., Wittchen K.D.;
RT   "Cloning and sequencing of the leu C and npr M genes and a putative spo IV
RT   gene from Bacillus megaterium DSM319.";
RL   Appl. Microbiol. Biotechnol. 41:344-351(1994).
CC   -!- FUNCTION: May have a role in completing stage IV of sporulation.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: To B.subtilis YqfD. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA52963.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP001982; ADF41364.1; -; Genomic_DNA.
DR   EMBL; X75069; CAA52963.1; ALT_TERM; Genomic_DNA.
DR   PIR; I40228; I40228.
DR   RefSeq; WP_013085053.1; NC_014103.1.
DR   AlphaFoldDB; P41028; -.
DR   EnsemblBacteria; ADF41364; ADF41364; BMD_4540.
DR   KEGG; bmd:BMD_4540; -.
DR   PATRIC; fig|592022.4.peg.4536; -.
DR   HOGENOM; CLU_050521_1_0_9; -.
DR   OMA; VRARVWY; -.
DR   Proteomes; UP000002365; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR010690; YqfD.
DR   Pfam; PF06898; YqfD; 1.
DR   PIRSF; PIRSF029895; SpoIV; 1.
DR   TIGRFAMs; TIGR02876; spore_yqfD; 1.
PE   4: Predicted;
KW   Membrane; Sporulation; Transmembrane; Transmembrane helix.
FT   CHAIN           1..395
FT                   /note="Putative stage IV sporulation protein"
FT                   /id="PRO_0000072075"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        49
FT                   /note="A -> R (in Ref. 2; CAA52963)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        75
FT                   /note="G -> R (in Ref. 2; CAA52963)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        92
FT                   /note="A -> R (in Ref. 2; CAA52963)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        104..105
FT                   /note="LL -> FV (in Ref. 2; CAA52963)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        249
FT                   /note="T -> N (in Ref. 2; CAA52963)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   395 AA;  45378 MW;  0022DEEED06A300A CRC64;
     MKNGWTNFVI GTVRIRIVGK GIERFLNNCV RQQIMISNVH KVDGQLATAT ILLKDVKKIR
     ILIRNADCKI YFIRGRGFPF LTKRVIKNSG FALGFLSFFI ILGLLSNMVW KVEISGAEPQ
     TEHQMTKQLA KIGVKRGEFQ FLLESPEKIQ RYLTDNMNNI TWVGVEVRGT SYHFQVVEKN
     EPKPQQKTPY QHLIAKKKAI ITNLFVEKGQ PLVKVNDFVN EGEVLVSGII GNEKNKKVVA
     AKGKVYGETW YKSEVEVPLK TDFQVLTGNG YTKHYLDFQA FKMPLWAFNK EKYTSKVTEK
     VEHPLYFFKW KLPLSYEKVA VREEQNSQRV YSKQEAVEKA LEIGRKKLLS TLGEDAKIKG
     EKVLHQEQDN GKVRLSIHYQ VIENIANTQP IIQGD