SP5AF_BACSU
ID SP5AF_BACSU Reviewed; 493 AA.
AC P31845;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 4.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Stage V sporulation protein AF;
GN Name=spoVAF; OrderedLocusNames=BSU23390;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 410-438.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-493.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=7934829; DOI=10.1111/j.1365-2958.1993.tb02670.x;
RA Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P.;
RT "The organization of the Bacillus subtilis 168 chromosome region between
RT the spoVA and serA genetic loci, based on sequence data.";
RL Mol. Microbiol. 10:385-395(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 223-493.
RX PubMed=1368705; DOI=10.1271/bbb1961.55.1615;
RA Yamamoto J., Shimizu M., Yamane K.;
RT "Molecular cloning and analysis of nucleotide sequence of the Bacillus
RT subtilis lysA gene region using B. subtilis phage vectors and a multi-copy
RT plasmid, pUB110.";
RL Agric. Biol. Chem. 55:1615-1626(1991).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-82.
RX PubMed=3926949; DOI=10.1099/00221287-131-5-1091;
RA Fort P., Errington J.;
RT "Nucleotide sequence and complementation analysis of a polycistronic
RT sporulation operon, spoVA, in Bacillus subtilis.";
RL J. Gen. Microbiol. 131:1091-1105(1985).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GerABKA family. {ECO:0000305}.
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DR EMBL; D84432; BAA12661.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14271.2; -; Genomic_DNA.
DR EMBL; L09228; AAA67472.1; -; Genomic_DNA.
DR EMBL; D90189; BAA14210.1; -; Genomic_DNA.
DR EMBL; M15349; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A69715; A69715.
DR RefSeq; NP_390220.2; NC_000964.3.
DR RefSeq; WP_003230471.1; NZ_JNCM01000036.1.
DR RefSeq; WP_009967659.1; NZ_CM000487.1.
DR AlphaFoldDB; P31845; -.
DR SMR; P31845; -.
DR STRING; 224308.BSU23390; -.
DR TCDB; 9.A.11.1.1; the dipicolinic acid transporter (dpa-t) family.
DR PRIDE; P31845; -.
DR EnsemblBacteria; CAB14271; CAB14271; BSU_23390.
DR GeneID; 938936; -.
DR KEGG; bsu:BSU23390; -.
DR PATRIC; fig|224308.179.peg.2548; -.
DR eggNOG; COG0697; Bacteria.
DR OMA; NTPYLWP; -.
DR PhylomeDB; P31845; -.
DR BioCyc; BSUB:BSU23390-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009847; P:spore germination; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR004995; Spore_Ger.
DR Pfam; PF03323; GerA; 1.
DR PIRSF; PIRSF005690; GerBA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Sporulation; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..493
FT /note="Stage V sporulation protein AF"
FT /id="PRO_0000164019"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 410..438
FT /note="NKMSRLVLMILVALFHIKGLVIGFTVLII -> TNEPSCPHDTRCFISYKRA
FT RHRIYSANY (in Ref. 1; BAA12661 and 4; AAA67472)"
FT /evidence="ECO:0000305"
FT CONFLICT 410..438
FT /note="NKMSRLVLMILVALFHIKGLVIGFTVLII -> TNEPSCPHDTRCFISYKRA
FT RHRLYSANY (in Ref. 5; BAA14210)"
FT /evidence="ECO:0000305"
FT CONFLICT 468
FT /note="R -> P (in Ref. 5; BAA14210)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 493 AA; 55473 MW; 5357B730F91361CE CRC64;
MPDHKEEKIR VYRNPAKNEE YFKNRVGMGT SYDVGVRKLT ILDKEIQLYY LNGLCDTAYI
IHLMRELVAI NNRKEDPDEL VDIVENRLLN AQVEKVKTLD ETTDQVLSGL VAVIVEGAGF
AFIIDVRSYP GRNPEEPDTE KVVRGARDGF VENIVVNTAL LRRRIRDERL RVKMTKVGER
SKTDLSICYI EDIADPDLVE IVEKEIASID VDGLTMADKT VEEFIVNQSY NPFPLVRYTE
RPDVAANHVL EGHVIIIVDT SPSVIITPTT LFHHVQHAEE YRQAPSVGTF LRWVRFFGIL
ASTLFLPIWF LFVLQPDLLP DNMKFIGLNK DTHIPIILQI FLADLGIEFL RMAAIHTPTA
LSTAMGLIAA VLIGQIAIEV GLFSPEVILY VSLAAIGTFT TPSYELSLAN KMSRLVLMIL
VALFHIKGLV IGFTVLIIAM ASIKSLQTPY LWPLIPFNGK ALWQVLVRTA KPGAKVRPSI
VHPKNRLRQP TNS
