SP5B_BACSU
ID SP5B_BACSU Reviewed; 518 AA.
AC Q00758;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Stage V sporulation protein B;
DE AltName: Full=Stage III sporulation protein F;
GN Name=spoVB; Synonyms=spoIIIF; OrderedLocusNames=BSU27670;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1744050; DOI=10.1128/jb.173.24.7942-7949.1991;
RA Popham D.L., Stragier P.;
RT "Cloning, characterization, and expression of the spoVB gene of Bacillus
RT subtilis.";
RL J. Bacteriol. 173:7942-7949(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Tosato V., Bolotin A., Bertani I., Valentino I., Bruschi C.V.;
RT "A 17.8 kb segment in the spoVB-nadC region of the Bacillus subtilis 168
RT chromosome: sequencing and ruv operon identification.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Involved, directly or indirectly, in spore cortex
CC biosynthesis. Affects only indirectly the expression of late
CC sporulation genes.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: SpoVB transcription takes place during the second
CC hour of sporulation. It may be transcribed mainly, if not only, in the
CC mother cell. Indeed, it is required only in the mother cell.
CC -!- SIMILARITY: Belongs to the polysaccharide synthase family.
CC {ECO:0000305}.
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DR EMBL; M76666; AAA22806.1; -; Genomic_DNA.
DR EMBL; Y15896; CAB75337.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14727.1; -; Genomic_DNA.
DR PIR; I39989; I39989.
DR RefSeq; NP_390645.1; NC_000964.3.
DR RefSeq; WP_004398529.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; Q00758; -.
DR SMR; Q00758; -.
DR STRING; 224308.BSU27670; -.
DR TCDB; 2.A.66.2.14; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR PaxDb; Q00758; -.
DR PRIDE; Q00758; -.
DR DNASU; 938022; -.
DR EnsemblBacteria; CAB14727; CAB14727; BSU_27670.
DR GeneID; 938022; -.
DR KEGG; bsu:BSU27670; -.
DR PATRIC; fig|224308.179.peg.3006; -.
DR eggNOG; COG2244; Bacteria.
DR InParanoid; Q00758; -.
DR OMA; LYYFQGP; -.
DR PhylomeDB; Q00758; -.
DR BioCyc; BSUB:BSU27670-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR CDD; cd13124; MATE_SpoVB_like; 1.
DR InterPro; IPR024923; PG_synth_SpoVB.
DR InterPro; IPR029303; Polysacc_synt_C.
DR InterPro; IPR002797; Polysacc_synth.
DR InterPro; IPR014249; Spore_V_B.
DR Pfam; PF01943; Polysacc_synt; 1.
DR Pfam; PF14667; Polysacc_synt_C; 1.
DR PIRSF; PIRSF038958; PG_synth_SpoVB; 1.
DR TIGRFAMs; TIGR02900; spore_V_B; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Sporulation; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..518
FT /note="Stage V sporulation protein B"
FT /id="PRO_0000166451"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 518 AA; 56079 MW; 2AE0A0F73FC4EE2B CRC64;
MAKQTFLKGT LILIAAGMVT RMLGFVNRVV IARFIGEEGV GLYMMAAPTF FLATTLTQFG
LPVAISKLVA EASARGDHQK TKNILVMSLT ITGVLSLIFT PLFLFFAPVM AETMLTDKRT
LYPLLAITPV VPIIAISSVL RGYFQGKQNM NPLAMSQVLE QVVRISLVAV CTTIFLPYGI
EYAAAGAMLS SVAGELASLL YLFVCFKYKK TIKIRKHFLQ SIKNGKQTFT QLMSVSLPTT
GSRFIGNLSW FFEPIVVAQS LAIAGVATVA ATKQYGELTG FAMTLLTLPS FITYSLSTAL
VPAISEGMEQ KKLQVVEYRL EQAMRLCLLS GGISVVILFV FADELMRVMY GSSGAAVFIK
VMAPFFLLYY FQGPLQAVLQ ALNLAGAAMM NSLIGALVKT GLIFVLATRP SLGIMGAALA
IVTGMVLVTL LHAATVSKVL PISIKIKEYA LSFAVIVICG FISSAIKQYI SFGASEAVNL
AGWIAASAAI YMILLLVFRL IKKDELRRIP IIGRLIIR
