ID   SP63_STRPU              Reviewed;         470 AA.
AC   Q07929; P98117;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=63 kDa sperm flagellar membrane protein;
DE   Flags: Precursor;
OS   Strongylocentrotus purpuratus (Purple sea urchin).
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Camarodonta; Echinidea; Strongylocentrotidae;
OC   Strongylocentrotus.
OX   NCBI_TaxID=7668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8509450; DOI=10.1083/jcb.121.6.1291;
RA   Mendoza L.M., Nishioka D., Vacquier V.D.;
RT   "A GPI-anchored sea urchin sperm membrane protein containing EGF domains is
RT   related to human uromodulin.";
RL   J. Cell Biol. 121:1291-1297(1993).
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane;
CC       Lipid-anchor, GPI-anchor. Note=Attached to the flagellar membrane by a
CC       GPI-anchor.
CC   -!- TISSUE SPECIFICITY: Sperm.
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DR   EMBL; M99584; AAA30029.1; -; mRNA.
DR   PIR; A40697; A40697.
DR   RefSeq; NP_999632.1; NM_214467.1.
DR   AlphaFoldDB; Q07929; -.
DR   EnsemblMetazoa; NM_214467; NP_999632; LOC373185.
DR   GeneID; 373185; -.
DR   KEGG; spu:373185; -.
DR   eggNOG; ENOG502RTRX; Eukaryota.
DR   HOGENOM; CLU_589672_0_0_1; -.
DR   OMA; CTIASTH; -.
DR   OrthoDB; 1023611at2759; -.
DR   PhylomeDB; Q07929; -.
DR   Proteomes; UP000007110; Unassembled WGS sequence.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031514; C:motile cilium; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   Gene3D; 3.30.70.960; -; 1.
DR   InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_site.
DR   InterPro; IPR018097; EGF_Ca-bd_CS.
DR   InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR   InterPro; IPR000082; SEA_dom.
DR   InterPro; IPR036364; SEA_dom_sf.
DR   Pfam; PF07645; EGF_CA; 1.
DR   Pfam; PF01390; SEA; 1.
DR   SMART; SM00181; EGF; 3.
DR   SMART; SM00179; EGF_CA; 2.
DR   SMART; SM00200; SEA; 1.
DR   SUPFAM; SSF57184; SSF57184; 1.
DR   SUPFAM; SSF82671; SSF82671; 1.
DR   PROSITE; PS00010; ASX_HYDROXYL; 1.
DR   PROSITE; PS01186; EGF_2; 2.
DR   PROSITE; PS50026; EGF_3; 2.
DR   PROSITE; PS01187; EGF_CA; 1.
DR   PROSITE; PS50024; SEA; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cell projection; Cilium; Disulfide bond; EGF-like domain;
KW   Flagellum; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..446
FT                   /note="63 kDa sperm flagellar membrane protein"
FT                   /id="PRO_0000007735"
FT   PROPEP          447..470
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000007736"
FT   DOMAIN          41..80
FT                   /note="EGF-like 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DOMAIN          81..205
FT                   /note="SEA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00188"
FT   DOMAIN          202..250
FT                   /note="EGF-like 2; calcium-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DOMAIN          249..292
FT                   /note="EGF-like 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   LIPID           446
FT                   /note="GPI-anchor amidated glycine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        78
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        170
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        219
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        322
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        45..57
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        50..66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        68..79
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        206..220
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        214..229
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        231..249
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        253..265
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        258..277
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        279..291
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
SQ   SEQUENCE   470 AA;  51101 MW;  4D421BC147D0D1D6 CRC64;
     MFCHLHCMLV VFSLLLTLTG SFVNAQTTEV VTEITATTAD PPDPCASNPC TIASTHCVAA
     GESHTCECRP GYFETNGNCT VAQQFAGSFS VTQVGGSNVL YSADLADTDS AAFASLAADV
     EDALDTVYQA STMADIYLGS EVWGVPEWLY RGRLHVLFAT EDAGQPVLVN STDATEAFTT
     ALAAEAANLG ITIDDSTITV SDFDECASAD DNDCDPNANC TNTAGSFTCE CDTELYDNSP
     NTEEPGRVCI APCDPGLCTR PNEICNNGGT IEDDNLCKCI EGYDYTQYGD CDPMARSTDF
     RCYHCEDSID NVKCNGRMES ENGTARQCPN PTDTCYQTIQ MNPEGDGFMI RKGCMNLEDC
     YDLLYSYEAD PAKASCFEYI FPYGQDTPPG PGVQCHYCCS EYFPLDLCNY DSIHFIYGTP
     RINSWDPRMN WDLSMNLDAT EEPESGSQRH LPVCGVLSLV VTTLLALMLH